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Oil-tea disease-resistant bacterial strain and application thereof

A technology for Camellia oleifera anthracnose and strains, applied in the directions of application, bacteria, fungicides, etc., can solve the problems of weak technology, few biological pesticides for preventing and controlling Camellia oleifera anthracnose, etc., and achieves good development and application prospects, easy industrial production, and easy preservation. Effect

Active Publication Date: 2011-08-17
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The isolation and screening of biocontrol strains with antagonistic effect on Camellia oleifera anthracnose from Camellia oleifera forest land is of great significance to the prevention and control of Camellia oleifera anthracnose, but the relevant technology is still weak or even blank, and it has been able to effectively control Camellia oleifera anthracnose for more than 20 years. There are few reports on antagonistic bacteria, and there are few reports on biopesticides for the prevention and treatment of camellia oleifera anthracnose

Method used

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  • Oil-tea disease-resistant bacterial strain and application thereof
  • Oil-tea disease-resistant bacterial strain and application thereof
  • Oil-tea disease-resistant bacterial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The separation of embodiment 1 bacteria

[0027] Collect soil samples from camellia oleifera forest land, shovel off about 5cm of topsoil, and then take samples, and put the samples into ziplock bags prepared in advance. In the laboratory, add 10g of soil sample into a 250ml Erlenmeyer flask containing 90ml of normal saline, shake it well, absorb 1ml of the supernatant, transfer it to a sterile test tube containing 9ml of normal saline, and dilute to 10 -7 dilution, and press 10 -7 -10 -3 The order of the dilutions is to draw 0.1ml respectively on the pre-prepared beef extract peptone medium to spread on the plate, repeat 3 times for each dilution, put it in the incubator for incubation at 30°C after spreading evenly, and record the amount of bacteria after 48 hours. They were selected according to the colony shape, color and size, purified and stored on the plate, and 5 batches were separated in total.

Embodiment 2

[0028] Example 2 Screening of antagonistic bacteria

[0029] Preliminary screening: use the confrontation culture method. Screening of antagonistic bacteria against pathogenic fungi. Put the 6mm-diameter cake of Camellia anthracnose bacteria in the center of the plate, then use the inoculation ring to pick up the suspension of the bacteria to be tested and inoculate 2 to 3 kinds of bacteria equidistantly (3cm from the center of the plate), and put them in the incubator Incubate in the dark at 25°C, observe and record the presence and size of the inhibition zone after 4 days, and repeat 3 times in total. Select 10 strains with a bacteriostatic zone width greater than or equal to 4mm for re-screening, followed by Z5, Z12, Z17, Z20, Z26, Z30, Z31, Z47, Z52, Z66 for re-screening (see Table 1).

[0030] Table 1 Antibacterial effect of isolated bacteria on Camellia oleifera anthracnose

[0031]

[0032]

[0033] Re-screening: use fermentation method. After the 10 initially...

Embodiment 3

[0037] Example 3 The inhibitory effect of Bacillus brevis Z26 on the hyphae of camellia oleifera anthracnose

[0038] Put the camellia anthracnose bacteria cake with a diameter of 6mm in the center of the PDA plate of the potato sucrose medium, then inoculate Bacillus brevis Z26 at an equal distance of 3cm, repeat three times, cultivate at 25-28°C, and measure the diameter of the inhibition zone after 5 days. It can be seen from the figure that Bacillus brevis Z26 has a strong antibacterial effect on Camellia oleifera anthracnose bacteria, among which image 3 : Bacteriostatic effect of Bacillus brevis Z26 on Camellia oleifera anthracnose.

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Abstract

The invention discloses an oil-tea disease-resistant bacterial strain and an application thereof, in particular relates to an oil-tea anthracnose-resistant bacterial strain belonging to the technical field of plant protection. The bacterial strain is Bacillus brevis Z26, and the preservation number of the bacterial strain is China center for type culture collection (CCTCC) No: M20110113. The oil-tea disease-resistant bacterial strain is obtained by first screening by means of the a confrontation test and second screening by means of liquid fermentation liquid , can be effectively preventing and curing various plant diseases such as oil-tea anthracnose, oil-tea root rot, oil-tea half mad, citrus anthracnose, citrus root rot, aloe root rot and the like, particularly can be used for effectively restraining the growth of oil-tea anthracnose germ, is high in prevention efficiency, is good in environment security, and is very good in development and application foreground.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a biocontrol bacterial strain for preventing and treating main diseases of camellia oleifera, in particular to a strain of Bacillus brevis Z26 for efficiently preventing and treating anthracnose of camellia oleifera and its application. Background technique [0002] Camellia oleifera (Camellia oleifera) is a unique edible oil tree species in my country and one of the four largest woody oil tree species in the world. It has a history of utilization and cultivation for more than 2,000 years. It has wide adaptability and strong stress resistance, and is suitable for planting in vast red soil hilly low areas. It mainly grows in the south, mainly in Hunan, Jiangxi, and Guangxi. Among them, Hunan Province is the largest camellia oleifera producing area and the largest camellia oil consumption area in China. [0003] Camellia oleifera is full of treasures and has high economic value. A...

Claims

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Application Information

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IPC IPC(8): A01P3/00A01N63/02C12R1/08C12N1/20
Inventor 刘君昂宋光桃周国英李琳李河何苑皞
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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