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Method for measuring high and low-density lipoprotein cholesterol content of animal meat

A high-density lipoprotein and low-density lipoprotein technology, which is used in measurement devices, instruments, scientific instruments, etc.

Inactive Publication Date: 2011-08-17
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no standard method for determining the content of high and low-density lipoprotein cholesterol in livestock and poultry meat in China. Some scientific researchers often refer to the method of measuring high and low-density lipoprotein cholesterol in blood for reference. Homogenize with a certain proportion of normal saline, then centrifuge to take the supernatant, and measure the content of the two kinds of cholesterol in the supernatant

Method used

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  • Method for measuring high and low-density lipoprotein cholesterol content of animal meat
  • Method for measuring high and low-density lipoprotein cholesterol content of animal meat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Determination method of high-density lipoprotein cholesterol in livestock and poultry meat:

[0034] 1. Reagents used

[0035] Potassium hydroxide (analytical pure), absolute ethanol (analytical pure), anhydrous ether (analytical pure), anhydrous sodium sulfate (analytical pure), distilled water, phosphotungstic acid (analytical pure), magnesium chloride (analytical pure), cholesterol (Standard), High Purity Nitrogen

[0036] 2. Operation method and steps

[0037] (1) Saponification

[0038] Weigh 0.5-1.0 g of sample, put it in a 100-ml stoppered colorimetric tube, add 10 ml of 1mol / L potassium hydroxide solution, 10 ml of absolute ethanol, mix well, install a condenser tube, Slowly saponify on a water bath at ℃ until the solution is clear (about 1 hour), then cool with running water.

[0039] (2) Cholesterol extraction

[0040] Transfer the saponified solution of the sample into a 50ml separating funnel, add 10ml of diethyl ether to mix evenly, let it stand for la...

Embodiment 2

[0063] Determination method of low-density lipoprotein cholesterol in livestock and poultry meat:

[0064] 1. Reagents used

[0065] Potassium hydroxide (analytical pure), absolute ethanol (analytical pure), anhydrous ether (analytical pure), anhydrous sodium sulfate (analytical pure), distilled water, sodium citrate (analytical pure), heparin, cholesterol (standard) , high-purity nitrogen

[0066] 2. Operation method and steps

[0067] (1) Saponification

[0068] Weigh 0.5-1.0 g of sample, put it in a 100-ml stoppered colorimetric tube, add 10 ml of 1mol / L potassium hydroxide solution, 10 ml of absolute ethanol, mix well, install a condenser tube, Slowly saponify on a water bath at ℃ until the solution is clear (about 1 hour), then cool with running water.

[0069] (2) Cholesterol extraction

[0070] Transfer the saponified solution of the sample into a 50ml separating funnel, add 10ml of diethyl ether to mix evenly, let it stand for layering, put the water layer into th...

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Abstract

The invention discloses a method for measuring high and low-density lipoprotein cholesterol content of animal meat. The method comprises the following steps of: saponifying lipid in the animal meat by using potassium hydroxide and absolute ethanol, extracting cholesterol serving as an unsaponifiable substance by using absolute ethyl ether, then separating out the high-density lipoprotein cholesterol in the cholesterol by using a phosphotungstic acid-magnesium chloride precipitant, separating out the low-density lipoprotein cholesterol in the cholesterol by using a sodium citrate-heparin precipitant, finally measuring the peak areas of the high-density lipoprotein cholesterol and the low-density lipoprotein cholesterol by using a gas chromatograph, and calculating the content of the high and low-density lipoprotein cholesterol content by using the peak areas. The high and low-density lipoprotein cholesterol content of the animal meat can be accurately and quantitatively measured by adopting the method, and the content value measured by adopting a medicinal method in the animal muscular tissue transudate is not total content in the muscle. Therefore, the invention provides an accurate and reliable method for accurately evaluating the functional animal meat.

Description

technical field [0001] The invention relates to a method for determining the content of high-density lipoprotein cholesterol and low-density lipoprotein cholesterol in livestock and poultry meat. Background technique [0002] Cholesterol is divided into high-density lipoprotein cholesterol and low-density lipoprotein cholesterol. High-density lipoprotein cholesterol can reduce the risk of coronary heart disease and is called "good" cholesterol; low-density lipoprotein cholesterol can increase the risk of coronary artery disease Heart disease risk, known as "bad" cholesterol. There are two sources of cholesterol in the human body, one is endogenous synthesis, and the other is from animal foods. In recent years, with the improvement of living standards, the proportion of animal food in people's diet has gradually increased, and excessive intake of exogenous cholesterol has induced various cardiovascular diseases. For this reason, scientific research workers have developed li...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 刘雪兰井庆川魏祥法石天虹武彬阎佩佩刘涛刘瑞亭
Owner POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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