Application of waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn grass
A compound bacterial agent and composting technology, which is applied in the direction of bacteria, fungi, organic fertilizers, etc., can solve the problems of ambiguity and complexity of composting bacteria symbiosis and mutual influence relationship
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Embodiment 1
[0074] The screening methods of the three strains are as follows:
[0075] (1) Enrichment: Weigh 10g of the collected compost sample and place it in a sterile Erlenmeyer flask, add 100ml of sterile water and glass beads and shake evenly, then take 10ml of the suspension in a Erlenmeyer flask containing 100ml of enrichment medium , cultured on a shaking table at a suitable temperature for 3 days;
[0076] (2) Preliminary screening: After diluting the enriched culture solution in a concentration gradient, spread it on the separation and screening medium, culture it upside down at a constant temperature, observe the size of the transparent circle, and select the colony with a large ratio of transparent circle / colony diameter carry out re-screening;
[0077] (3) Re-screening: inoculate the strains obtained from the initial screening into the corresponding shake flask culture medium, and cultivate them under the conditions of suitable temperature and shaking table speed. The OD va...
Embodiment 2
[0083]The selected lawn plant seeds have full grains and uniform size. The soil used is the garden soil of Tianjin Normal University. Its physical and chemical properties are: pH 7.44, saturated water content 0.58 ml / g, organic matter 4.68%, total nitrogen 0.21%, available phosphorus 22.03 mg / kg, and total potassium 45.61 g / kg. After collection, remove plant residues, gravel and other sundries, pass through a 2mm sieve, and sterilize at 121°C for 30 minutes for later use. Sow ryegrass and tall fescue seeds respectively in petri dishes (diameter 9 cm) filled with 60 g of sterilized soil, 150 per dish. Sowing with a fixed number of seeds, the purity is 100%; the germination rate of the seeds is about 85%. Provide uniform and quantitative water every day to maintain a good water condition in the culture medium, and change the position of each culture container frequently to ensure consistent light. When the plant grows to the tillering stage, add the compost microbial agent (10...
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