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Application of waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn grass

A compound bacterial agent and composting technology, which is applied in the direction of bacteria, fungi, organic fertilizers, etc., can solve the problems of ambiguity and complexity of composting bacteria symbiosis and mutual influence relationship

Inactive Publication Date: 2011-09-07
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the research process of compound microbial agents, the identification and classification of microbial species in compost, the symbiosis and mutual influence relationship between compost strains are ambiguous and complex, and the application of compost microbial agents to plants under normal conditions In terms of the effect on growth, there are few related studies reported
However, the identification and classification of microbial species in compost, the symbiosis and mutual influence relationship between compost strains are fuzzy and complex, and the extraction, screening and preparation of compost compound beneficial microbial agents from municipal solid waste compost have a great impact on the growth of lawn plants. , Physiological properties of the impact of research has not been reported

Method used

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  • Application of waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn grass
  • Application of waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn grass
  • Application of waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn grass

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Experimental program
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Effect test

Embodiment 1

[0074] The screening methods of the three strains are as follows:

[0075] (1) Enrichment: Weigh 10g of the collected compost sample and place it in a sterile Erlenmeyer flask, add 100ml of sterile water and glass beads and shake evenly, then take 10ml of the suspension in a Erlenmeyer flask containing 100ml of enrichment medium , cultured on a shaking table at a suitable temperature for 3 days;

[0076] (2) Preliminary screening: After diluting the enriched culture solution in a concentration gradient, spread it on the separation and screening medium, culture it upside down at a constant temperature, observe the size of the transparent circle, and select the colony with a large ratio of transparent circle / colony diameter carry out re-screening;

[0077] (3) Re-screening: inoculate the strains obtained from the initial screening into the corresponding shake flask culture medium, and cultivate them under the conditions of suitable temperature and shaking table speed. The OD va...

Embodiment 2

[0083]The selected lawn plant seeds have full grains and uniform size. The soil used is the garden soil of Tianjin Normal University. Its physical and chemical properties are: pH 7.44, saturated water content 0.58 ml / g, organic matter 4.68%, total nitrogen 0.21%, available phosphorus 22.03 mg / kg, and total potassium 45.61 g / kg. After collection, remove plant residues, gravel and other sundries, pass through a 2mm sieve, and sterilize at 121°C for 30 minutes for later use. Sow ryegrass and tall fescue seeds respectively in petri dishes (diameter 9 cm) filled with 60 g of sterilized soil, 150 per dish. Sowing with a fixed number of seeds, the purity is 100%; the germination rate of the seeds is about 85%. Provide uniform and quantitative water every day to maintain a good water condition in the culture medium, and change the position of each culture container frequently to ensure consistent light. When the plant grows to the tillering stage, add the compost microbial agent (10...

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Abstract

The invention discloses application of a prepared domestic waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn plants and promoting synthesis of chlorophyll. The composite bacterial agent consists of three strains, namely bacillus subtilis, actinomycetes and saccharomycetes in volume ratio of 1 to 1 to 1. Experiments show that seed germination rate, seedling height, aboveground dry weight, root weight, chlorophyll a content and chlorophyll b content of festuca arundinacea are 13.41 percent, 58.92 percent, 72.86 percent, 100 percent, 68.92 percent and 37.61 percent higher than contrast values respectively and the seed germination rate, the seedling height, the aboveground dry weight, the root weight, the chlorophyll a content and the chlorophyll b content of ryegrass are 15.11 percent, 34.44 percent, 71.74 percent, 100 percent, 66.89 percent and 33.41 percent higher than contrast values respectively after being treated by inoculating the three-strain composite bacterial agent. Technical effects show that the high-efficiency compost composite bacterial agent provides proper growth conditions and nutrients for plants, meets requirements on growth conditions required by the plants and therefore contributes to growth of the ryegrass and the festuca arundinacea.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to the preparation of a high-efficiency composting compound bacterial agent by using beneficial bacteria extracted from urban domestic garbage composting as raw materials. More specifically, it is the application of the preparation of compound bacterial agent in enhancing the photosynthetic ability of lawn plants and promoting the synthesis of chlorophyll. Background technique [0002] Composting is a biochemical process that uses microorganisms (bacteria, actinomycetes, fungi, etc.) widely present in nature or commercial strains to controlly promote the transformation of biodegradable organic matter into stable humus (humic substance, HS). . This process is also part of the ecological process on the earth's surface, which participates in the cycle of matter and energy on the earth's surface. [0003] Under artificial control conditions, the organic matter in solid ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C05F11/08C12R1/125C12R1/04
Inventor 多立安赵树兰王晶晶
Owner TIANJIN NORMAL UNIVERSITY
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