Combined extraction method of blackberry seed oil and anthocyanin in blackberry juice production byproducts

A technology of combined extraction and by-products, applied in the production of fat oil/fat, production of fat, and refining of fat oil/fat, etc., can solve the problems of environmental pollution, waste of resources, etc., achieve low economic benefits, reduce production and emissions, and achieve efficient utilization Effect

Active Publication Date: 2011-09-07
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

At present, these processing by-products are treated as waste, which

Method used

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Example Embodiment

[0016] Take 500 kg of by-products after squeezing the fresh blackberry fruit juice, crush it with a multifunctional pulverizer (WF-40B), add 1000 kg of drinking water, adjust the pH to 4.5 with 1mol / L sodium hydroxide solution, and add 750 g cellulose-half Cellulose-starch complex enzyme cellulose (20000u / g cellulase, 30000u / g hemicellulase and 10000u / ml mid-temperature amylase mixed according to the ratio of activity 5:3:1), enzymatically hydrolyze at 55℃ for 70min, 90 Incubate at ~95°C for 40 minutes to inactivate the enzyme to obtain a suspension;

[0017] Add 700L of No. 6 solvent (Nantong Tianrun Special Solvent Oil Co., Ltd.) to the suspension, seal and stir for 50 minutes, stop stirring, and stand for 1.5 hours, then separate into the upper solvent phase, the lower solid-liquid mixed water phase, and the upper solvent phase;

[0018] The upper solvent phase is decompressed to recover the solvent to obtain crude oil. 3% by mass of activated clay (Zibo Zhengtian Chemical Co.,...

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Abstract

The invention provides a combined extraction method of blackberry seed oil and anthocyanin in blackberry juice production byproducts, belonging to the technical field of fine and deep processing of agricultural products. The method comprises the following steps: crushing the blackberry juice production byproducts, and performing cellulose-hemicellulose-starch composite enzymatic hydrolysis; extracting with a No.6 solvent; steaming the oil layer to remove a solvent and refining to obtain blackberry essential oil; and performing extraction, chromatography and vacuum concentration on a water layer with high-pressure pulse assisting ethanol to obtain an anthocyanin concentrated solution. The method provided by the invention sufficiently utilizes the functional components in blackberry, reduces generation and discharge of waste residues to the greatest degree, realizes comprehensive utilization of blackberry, and obviously increases the economic value of blackberry.

Description

1. Technical field [0001] The invention is a joint extraction method of blackberry seed oil and anthocyanin in the by-products of blackberry juice production, which applies modern biotechnology to the production of seed oil and anthocyanins in the by-products of clear berry juice production, and is specially used for the production of by-products of clear berry juice. The comprehensive processing of products belongs to the technical field of deep processing of agricultural products. 2. Background technology [0002] my country currently produces 87,000 tons of blackberries, accounting for 13.7% of the world's total demand. Due to factors such as raw materials and labor in developed countries such as Europe and the United States, the production volume is gradually decreasing, and the production volume in developing and underdeveloped countries and regions is increasing year by year. In recent years, provinces such as Jiangsu, Shandong, Anhui, Sichuan, Heilongjiang and Jilin ...

Claims

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Application Information

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IPC IPC(8): C12P17/06C11B1/04C11B1/10C11B3/00C11B3/10
Inventor 单成俊周剑忠刘小莉李莹王英张丽霞黄开红黄自苏
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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