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HBV (hepatitis B virus) fluorescent quantitative PCR (polymerase chain reaction) detection kit

A hepatitis B virus and detection kit technology, applied in the field of medical detection, can solve the problems of poor specificity and inability to detect the eight genotypes of HBV, etc., and achieve improved detection sensitivity, monitoring the existence of false negatives, and good specificity Effect

Active Publication Date: 2011-09-07
SANSURE BIOTECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0012] The purpose of the present invention is to provide a hepatitis B virus fluorescent quantitative PCR detection kit, which solves the technical problem that the specificity of the hepatitis B detection kit in the prior art is poor and cannot detect all eight genotypes of HBV

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  • HBV (hepatitis B virus) fluorescent quantitative PCR (polymerase chain reaction) detection kit
  • HBV (hepatitis B virus) fluorescent quantitative PCR (polymerase chain reaction) detection kit
  • HBV (hepatitis B virus) fluorescent quantitative PCR (polymerase chain reaction) detection kit

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Embodiment Construction

[0037] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the claimed invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0038] In a specific embodiment of the present invention, a kind of hepatitis B virus fluorescent quantitative PCR detection kit is provided, comprising the following components:

[0039] (1) The probe HBV-P used to detect the hepatitis B virus target polynucleotide has the sequence of SEQ No.1, which is derived from the conserved region of the hepatitis B virus surface antigen S gene, and the sequence of SEQ No.1 is (synthesized by Invitrogen Company) :

[0040] SEQ No.1: 5'-CCTCTTCATCCTGCTGCTATGCCTCATTCTTCTTATTGG-3';

[0041] Preferably, the carboxyl end of SEQ No. 1 is labeled with FAM, and the hydroxyl end is modified with a BHQ1 quench...

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Abstract

The invention discloses an HBV (hepatitis B virus) fluorescent quantitative PCR (polymerase chain reaction) detection kit which comprises a probe for detecting the target polynucleotide, an upstream primer and a downstream primer for amplifying the target polynucleotide, a PCR buffer solution and DNA (deoxyribonucleic acid) polymerase, wherein the probe for detecting the target polynucleotide hasa SEQ No.1 sequence. As the probe for detecting the target polynucleotide has the SEQ No.1 sequence, the kit disclosed by the invention has good specificity; by using the kit provided by the invention, the HBV-DNA concentration in the unknown sample such as serum, plasma or milk can be quickly and accurately measured; and the kit has good specificity and can detect eight gene types of HBV.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a kit for detecting hepatitis B virus nucleic acid by fluorescent quantitative PCR. Background technique [0002] Immunological detection of hepatitis B virus (Hepatitis B Virus, HBV) is a traditional method for diagnosing HBV infection. It is generally believed that the presence of hepatitis B virus surface antigen (HBsAg) in serum or plasma indicates that hepatitis B virus has been infected, but the appearance of HBsAg cannot provide information on the replication activity of the virus in vivo. A single e-antigen (HBeAg) is not accurate enough as an immune index to judge whether the replication of hepatitis B virus is active or not. With the continuous development and clinical application of genetic diagnosis technology, the level of HBV-DNA in serum is considered to not only prove the existence of hepatitis B virus, but also reflect the activity of the virus. Rational drug u...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 戴立忠
Owner SANSURE BIOTECH
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