Preparation with double functions of stopping bleeding and sustainedly releasing granulocyte colony stimulating factor and preparation method thereof

A colony-stimulating factor, dual-action technology, which can be used in medical preparations containing active ingredients, extracellular fluid diseases, blood diseases, etc. Incomplete and other problems, to achieve the effects of rapid degradation, convenient administration, and good linearity

Inactive Publication Date: 2011-09-14
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are: severe burst release and incomplete release of granulocyte colony-stimulating factor drug, protein denaturation and inactivation due

Method used

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  • Preparation with double functions of stopping bleeding and sustainedly releasing granulocyte colony stimulating factor and preparation method thereof
  • Preparation with double functions of stopping bleeding and sustainedly releasing granulocyte colony stimulating factor and preparation method thereof
  • Preparation with double functions of stopping bleeding and sustainedly releasing granulocyte colony stimulating factor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Preparation and method of preparation with dual effects of hemostasis and sustained release of granulocyte colony-stimulating factor (G-CSF)

[0042] ①Take a piece of absorbable oxidized cellulose Surgicel (10cm*1cm) and unfold it;

[0043] ②Weigh 0.25mg of polylactic-glycolic acid (PLGA, molecular weight 47kD, lactic acid:glycolic acid=50:50), dissolve in 1.25mg of ethyl acetate for later use;

[0044] ③ Weigh 0.02 mg of dextran particles containing 0.01 mg of granulocyte colony-stimulating factor (the particle size of dextran particles is 5 μm ± 4 μm);

[0045] ④Put the granulocyte colony-stimulating factor dextran granules of ③ into the solution of ②, mix thoroughly and evenly, and obtain a suspension;

[0046] ⑤ Spread the suspension of ④ on the absorbable oxidized cellulose Surgicel with a small brush;

[0047] ⑥Dry ⑤ to get the finished product.

[0048] ⑦In vitro release curve see image 3 , showing good release kinetics.

[0049] ⑧Using the finis...

Embodiment 2

[0053] Example 2: Preparation and method of preparation with dual effects of hemostasis and sustained release of granulocyte colony-stimulating factor (G-CSF)

[0054] ①Take a piece of absorbable oxidized cellulose Surgicel (2cm*1cm) and unfold it;

[0055] ②Weigh 60 mg of polylactic-glycolic acid (PLGA, molecular weight 47kD, lactic acid: glycolic acid=65:35), dissolve in 300 mg of ethyl acetate for later use;

[0056] ③ Weigh 6 mg of dextran particles containing 1.5 mg of granulocyte colony-stimulating factor (the particle size of dextran particles is 5 μm ± 4 μm);

[0057] ④Put the granulocyte colony-stimulating factor dextran granules of ③ into the solution of ②, mix thoroughly and evenly, and obtain a suspension;

[0058] ⑤ Spread the suspension of ④ on the absorbable oxidized cellulose Surgicel with a small brush;

[0059] ⑥Dry ⑤ to get the finished product.

[0060] ⑦In vitro release curve see Figure 4 , showing good release kinetics.

[0061] ⑧Using the finished ...

Embodiment 3

[0064] Example 3: Preparation and method of preparation with dual effects of hemostasis and sustained release of granulocyte colony-stimulating factor (G-CSF)

[0065] ①Take a piece of absorbable oxidized cellulose Surgicel (2cm*1cm) and unfold it;

[0066] ②Weigh 1200mg of polylactic-glycolic acid (PLGA, molecular weight 47kD, lactic acid: glycolic acid=50:50), dissolve in 6000mg of ethyl acetate for later use;

[0067] ③ Weigh 60 mg of dextran particles containing 3 mg of granulocyte colony-stimulating factor (the particle size of dextran particles is 5 μm ± 4 μm);

[0068] ④Put the granulocyte colony-stimulating factor dextran granules of ③ into the solution of ②, mix thoroughly and evenly, and obtain a suspension;

[0069] ⑤ Spread the suspension of ④ on the absorbable oxidized cellulose Surgicel with a small brush;

[0070] ⑥Dry ⑤ to get the finished product.

[0071] ⑦In vitro release curve see Figure 5 , showing good release kinetics.

[0072] ⑧Using the finished ...

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Abstract

The invention discloses a preparation with double functions of stopping bleeding and sustainedly releasing a granulocyte colony stimulating factor and a preparation method thereof in the technical field of medicine preparation. The preparation consists of glucan particles containing the granulocyte colony stimulating factor, a degradable sustained/controlled release material and a hemostatic material framework. By combining surgical hemostatic gauze with a medicine sustained-release system consisting of the glucan particles containing the granulocyte colony stimulating factor and a degradable sustained/controlled release material coating, a medicine loading procedure is added in a hemostatic gauze production process in a preparation process; and the method is simple and practicable and is suitable for various medicines.

Description

technical field [0001] The invention relates to a preparation and a method in the technical field of medicine preparation, in particular to a preparation with dual functions of hemostasis and sustained release of granulocyte colony-stimulating factor (G-CSF) and a preparation method thereof. Background technique [0002] Although there are many ways to solve the problem of hemostasis, it is difficult to solve the regeneration of blood, often relying on blood transfusion, and blood transfusion is easy to be contaminated and cause serious infection. In the field of tumor treatment, especially the surgical treatment and chemotherapy of solid tumors, more blood loss is caused, which seriously affects the lives of patients. Neutropenia caused by cancer chemotherapy often requires frequent injections of granulocyte colony-stimulating factor. In order to solve the hemostasis and increase neutrophils caused by chemotherapy and surgical resection, hemostasis is often used to stop bl...

Claims

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Application Information

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IPC IPC(8): A61L15/44A61L15/28A61L15/32A61K38/19A61K47/34A61K47/36A61P7/00A61P7/04A61P7/06A61K47/10
Inventor 袁伟恩金拓吴飞胡振华郑瑞媛冯艳
Owner SHANGHAI JIAO TONG UNIV
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