Recombinant adenovirus expressing E0, E2 gene of classical swine fever virus

Abstract

The invention provides recombinant adenovirus expressing E0, E2 genes of classical swine fever virus, which is characterized in that the E0 gene of classical swine fever virus is inserted into Bgl II and Kpn I enzyme sites of a multiple cloning site at downstream of adenovirus CMV promoter, and a adenovirus CMV promoter and a CMV-E2 segment of the E2 gene of classical swine fever virus are inserted into Xho I and Xba I enzyme sites. The invention solves the problems of low yield and weak protective effect of the fusion tandem expression of the E0, E2 genes, allows an immunized swine to obtainan antibody against classical swine fever virus only by immunization once, and the production cost is half of that of conventional vaccines; the recombinant adenovirus belongs to non-replicative virus, and does not propagate in swine; the phenomenons of virus atavism and virulence enhancement due to the long-term inoculation of attenuated vaccine are solved. The recombinant adenovirus only comprises main protective genes of classical swine fever virus and does not comprises other genes of the virus, so no recombination with field strains occurs. After a swine is immunized by vaccine prepared by the recombinant adenovirus, the protective effect against live virus infection of classical swine fever virus with a lethal dose of 100 times of TCID 50 is 100%.

Description

technical field [0001] The invention relates to a recombinant adenovirus, in particular to a recombinant adenovirus capable of expressing the E0 gene and E2 gene of swine fever virus in the same adenovirus, and belongs to the field of biotechnology. Background technique [0002] Swine fever is a very contagious infectious disease that often causes devastating losses to the pig industry. At present, immunization is still the main measure for the prevention and treatment of swine fever, and it is a necessary means to eradicate swine fever and prevent wild virus from spreading to pigs without swine fever. No matter in the past, at present or in the future, swine fever vaccine plays an extremely important role in controlling and eliminating swine fever. Scholars from various countries have never given up on the research and development of swine fever vaccine. Traditional swine fever vaccines include inactivated vaccines and attenuated vaccines. Among them, inactivated vaccines ...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of virus regression and virulence enhancement caused by long-term vaccination of attenuated classical swine fever vaccine and the recombination between the attenuated vaccine strain and wild virus, as well as the low yield and weak protective effect of classical swine fever virus E0 and E2 gene fusion and tandem expression Problem, the present invention provides a recombinant adenovirus capable of expressing classical swine fever virus E0 and E2 genes in the same adenovirus

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