Method for producing peanut polysaccharides and peanut concentrated protein by using peanut meal
A technology of concentrating protein and peanut meal, which is applied in the direction of plant peptides and peptide sources, can solve the problems of easily destroying the structure of natural active polysaccharides, the complicated process of purifying and removing proteins, and the unfavorable physiological activity of polysaccharides, so as to improve the value of comprehensive utilization, development and utilization Economical, nutritious and functional
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Embodiment 1
[0029] Take 1g of crushed and refluxed peanut meal micropowder, add 25ml containing 0.004mol L -1 Mg 2+ In the aqueous solution, adjust the pH to 7.0, add 175 U / g of α-amylase and 105 U / g of cellulase, and perform enzymatic hydrolysis at 50°C for 60 minutes to obtain the enzymatic hydrolyzate. enzyme. Centrifuge the enzymatic hydrolyzate after deactivation at 3000r / min and 25°C for 10min, take the supernatant to obtain a solution containing peanut polysaccharide, and use the phenol-sulfuric acid method and 3,5-dinitrosalicylic acid method to determine Under this condition, the yield (dry basis ratio) of peanut polysaccharide was 12.16%. The precipitated residue after centrifugation was dried to obtain peanut protein concentrate, and the protein retention rate was 86.63%. Concentrate the supernatant (that is, the solution containing peanut polysaccharide) in a vacuum at 30°C to 1 / 4 of the original volume and then cool it down. Stand at ℃ for 8 hours to fully precipitate the...
Embodiment 2
[0031] Take 1g of crushed and refluxed peanut meal micropowder, add 15ml containing 0.003mol L -1 Ca 2+ In the aqueous solution, adjust the pH to 6.5, add 175U / g of α-amylase and 105U / g of cellulase, and enzymolyze at 50°C for 45min to obtain the enzymolyzed liquid, and extinguish it at 80°C after the enzymolysis is completed. enzyme. Centrifuge the enzymatic hydrolyzate after deactivation at 5000r / min and 4°C for 10min, take the supernatant to obtain a solution containing peanut polysaccharide, and use the phenol-sulfuric acid method and 3,5-dinitrosalicylic acid method to determine Under this condition, the yield (dry basis ratio) of peanut polysaccharide was 12.17%. The precipitated residue after centrifugation is dried to obtain concentrated peanut protein, and the protein retention rate is 94.07%. Concentrate the supernatant (i.e. the solution containing peanut polysaccharide) in a vacuum at 50°C to 1 / 10 of the original volume, then cool it down, and add ethanol with a...
Embodiment 3
[0033] Take 1g of crushed and refluxed peanut meal micropowder, add 25ml containing 0.003mol L -1 Zn 2+ In the aqueous solution, adjust pH=6.5, add cellulase with an enzyme amount of 105U / g, and enzymolyze at 70°C for 30 minutes; then add α-amylase with an enzyme amount of 175U / g to continue enzymatic hydrolysis for 45 minutes to obtain an enzymolyzed solution , inactivate the enzyme at 95°C after the enzymatic hydrolysis is completed. Centrifuge the enzymatic hydrolyzate after deactivation at 2000r / min and 4°C for 10min, take the supernatant to obtain a solution containing peanut polysaccharide, and use the phenol-sulfuric acid method and 3,5-dinitrosalicylic acid method to determine Under this condition, the yield (dry basis ratio) of peanut polysaccharide was 12.59%. The precipitated residue after centrifugation is dried to obtain peanut protein concentrate, and the protein retention rate is 98.59%. Concentrate the supernatant (that is, the solution containing peanut pol...
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