Method for using corn stalk fiber carrier to prepare immobilized yeast cells employed in kitchen waste fermentation for ethanol fuel production
A technology of corn stalk fiber and kitchen waste, which is applied in the field of bioengineering, can solve the problems of high cost and difficulty of kitchen waste, and achieve the effect of low cost, convenient operation and realization of harmlessness
Active Publication Date: 2011-09-28
安徽科聚环保新能源有限公司
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Problems solved by technology
[0006] The invention aims to solve the problems that it is difficult to dispose of kitchen waste reasonably and effectively and the cost of the existing method for preparing fuel ethanol is high
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Embodiment
[0024] Embodiment: a method of using corn stalk fiber as a carrier to prepare immobilized yeast cells and use them for fermentation of kitchen waste to produce fuel ethanol, comprising the following steps:
[0025] (1) Peel the dry corn stalks and cut them into small pieces. The size of each small piece is about 2cm×2cm. Weigh 500g of the small piece of straw and transfer it to 5L fermented with 1.5L 0.05M phosphate buffer (pH4.8). In the tank, add cellulase to carry out enzymolysis reaction at 40°C, the dosage of cellulase is 50FPU / g straw, and the enzymolysis time is 4h. After the reaction, rinse with tap water until the pH is neutral; figure 2 B is the picture of corn stalk fiber modified by cellulase taken by scanning electron microscope.
[0026] (2) Drying of the carrier: drying the prepared corn stalk carrier at 50° C. to a constant weight, ready for use;
[0027] (3) Cell pre-cultivation: the yeast was inserted into a 5L fermenter equipped with 2L of YPD medium, and...
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The invention discloses a method for using corn stalk fiber carrier to prepare immobilized yeast cells employed in kitchen waste fermentation for ethanol fuel production. According to the method, corn stalks modified by cellulase are taken as a carrier for preparing immobilized yeast cells. And biomass wastes in kitchen wastes are subjected to enzymolysis, centrifugation, adjustment of sugar content and pH value, and then inoculated with the immobilized yeast cells for anaerobic fermentation, and finally purified by distillation, thus obtaining industrial ethanol. In the invention, the method employing immobilized cells is characterized by convenient operation, low cost, high mass transfer efficiency, and realization of repeated batch fermentation and continuous fermentation. In addition, as kitchen wastes are taken as the raw material for producing ethanol fuel, the method provided in the invention is also characterized by inexpensive raw material, small investment and low cost. Moreover, harmlessness, reduction and recycle of kitchen wastes are realized.
Description
technical field [0001] The invention relates to the application technology of cell immobilization and liquid fermentation in the field of bioengineering, in particular to a cellulase-modified corn stalk used as a carrier to prepare immobilized cells, and use the immobilized cells to ferment kitchen waste to produce fuel ethanol Methods. Background technique [0002] Catering waste refers to food waste and catering leftovers from families, schools, institutions, public canteens and the catering industry, and is an important part of municipal solid waste. The amount of kitchen waste generated in my country is huge, and with the rapid expansion of cities, the increase of urban population and the improvement of people's living standards, the amount of kitchen waste generated is increasing. Although the composition, properties and production of kitchen waste vary due to socio-economic conditions, regional differences, living habits of residents, dietary structure, and seasonal c...
Claims
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IPC IPC(8): C12P7/08C10L1/02C12P5/02C12N11/12C12R1/865
CPCY02E50/343Y02E50/30
Inventor 姚建铭颜守保汪平超陈祥松吴金勇李俊胡战伟翟志伟叶建丰朱微微杨乃坤
Owner 安徽科聚环保新能源有限公司