Corn breeding method

A maize breeding and maize technology, applied in the fields of botanical equipment and methods, seed and rhizome treatment, plant genetic improvement, etc., can solve the problems of less radiation mutation breeding, low frequency of beneficial mutations, and difficulty in obtaining favorable mutations, etc. The effect of shortening breeding years, increasing correctness and efficiency

Inactive Publication Date: 2011-10-19
河南金苑种业股份有限公司
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Problems solved by technology

[0002] Radiation mutation breeding refers to the use of various rays, such as X-rays, γ-rays, neutrons, etc. to irradiate the seeds, plants or certain organs and tissues of crops to promote them to produce various variations, and then select the required heritable good variations , a breeding method for cultivating new elite varieties. At present, radiation mutation breeding has been widely used in self-breeding crops such as a variety of vegetables and wheat; the advantage of this breeding method is that it can increase the frequency of variation and enrich the species quality resources; the main problem of mutation breeding is that the frequency of beneficial mutations is still low, and the

Method used

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Embodiment 1

[0018] Embodiment 1: a kind of corn breeding method, it comprises the following steps:

[0019] Step 1. In the first year, according to the available maize germplasm resources, select the seeds of excellent maize bicyclic system or mixed populations as radiation materials, and use 60Co-γ rays to irradiate the radiation materials to obtain radiation-induced seeds; After the seeds of the ring system or mixed population are irradiated, various variations may occur, which greatly enriches the germplasm resources of maize;

[0020] Step 2, plant the radiation-induced mutation seeds in the field, observe and record in detail from the seedling stage, select target trait mutants, and then self-cross, single-ear threshing after mature harvest, to obtain the target mutant strain grains; but these The grains of the target mutant strains cannot be directly combined with hybrids, and multiple generations of self-selection are still required to obtain homozygous inbred lines before they can...

Embodiment 2

[0026] Embodiment 2: a kind of corn breeding method, it comprises the following steps:

[0027] Step 1. In 2007, we selected 1,500 seeds of the corn mixed population named Huangzaosi, which we had, and carried out radiation mutagenesis with 60Co-γ rays at a dose of 300Gy to obtain radiation-mutated seeds;

[0028] Step 2. In the summer of 2007, the radiation-induced mutation seeds were sown in a single seed in the Zhengzhou test base, and detailed observation and recording were carried out from the seedling stage, and 3 mutant strains with target traits were selected in total, and then selfed. After maturity and harvest, Threshing with a single ear number, a total of 866 grains were harvested, and the grains of the target mutant strain were obtained;

[0029] Step 3, in the summer of 2008, sow the grains of the target mutant strain by single seed number. After silking, use the inducer line as the male parent to pollinate the target mutant strain, that is, induce with the induc...

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Abstract

The invention provides a corn breeding method, comprising the following steps: radiating seeds of a second cycle corn or a population mixture, then planting, selecting target character variation strains for inbreeding, carrying out single ear threshing, planting again and inducing with an inducer, and selecting haploid grains with a purple color label on top of an aleurone layer, and without a purple label on embryonic tips; doubling the haploid grains, carrying out inbreeding on the successfully doubled plants, and collecting inbred maturing grains; planting the selfing maturing grains, selecting ears satisfying breeding object requirements for inbreeding, obtaining a new corn inbred line, and testing the new corn inbred line pollen with testing seeds; planting the testing combination, carrying out field identification and finishing breeding a new corn species. The method combines the haploid breeding and the mutation breeding to rapidly obtain a homozygous inbred line for assembling a hybrid combination, so as to reduce breeding time and raise correctness and efficiency of corn variety breeding.

Description

technical field [0001] The invention relates to a method for cultivating plant varieties, in particular to a method for breeding maize by combining ray radiation mutagenesis and haploid induction. Background technique [0002] Radiation mutation breeding refers to the use of various rays, such as X-rays, γ-rays, neutrons, etc. to irradiate the seeds, plants or certain organs and tissues of crops to promote them to produce various variations, and then select the required heritable good variations , a breeding method for cultivating new elite varieties. At present, radiation mutation breeding has been widely used in self-breeding crops such as a variety of vegetables and wheat; the advantage of this breeding method is that it can increase the frequency of variation and enrich the species quality resources; the main problem of mutation breeding is that the frequency of beneficial mutations is still low, and the direction and nature of variation are still difficult to control. T...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/06A01C1/00
Inventor 张中伟张国辉李永铭潘科峰黎刚
Owner 河南金苑种业股份有限公司
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