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Method for extracting garlic superoxide dismutase from garlic sheet processing waste water

A superoxide and waste water processing technology, applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., to achieve low production costs, strong market competitiveness, and reduce COD effects

Inactive Publication Date: 2011-10-19
SHANDONG SHUNBANG BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the extraction of garlic superoxide dismutase (SOD) from garlic processing wastewater by using molecular imprinting technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023]With multiple 400-mesh stainless steel screens as the supporting material and 200-mesh diatomite as the filter medium, the diatomite feed liquid is circulated through the pump, and a 5 mm thick diatomite filter layer is evenly coated on the stainless steel screen in advance. Then 10 cubic meters of garlic slice processing wastewater are filtered to remove large particles of impurities and colloidal substances in the garlic slice processing wastewater; the filtered garlic slice processing wastewater is put into a reactor, heated to 75°C, and the heating time is 20 minutes to make garlic Denature and flocculate the miscellaneous proteins in the garlic slice processing wastewater, cool to 30°C, and then filter through diatomaceous earth hanging film to remove the miscellaneous proteins in the garlic slice processing wastewater; then filter and clarify the garlic slice processing wastewater, and pump it into A resin column equipped with 1,000 kg of superoxide dismutase (SOD) ...

Embodiment 2

[0028] With multiple 400-mesh stainless steel screens as the supporting material and 200-mesh diatomite as the filter medium, the diatomite feed liquid is circulated through the pump, and a 10 mm thick diatomite filter layer is evenly coated on the stainless steel screen in advance. Then 10 cubic meters of garlic slice processing wastewater are filtered to remove large particles of impurities and colloidal substances in the garlic slice processing wastewater; the filtered garlic slice processing wastewater is put into a reactor, heated to 65°C, and the heating time is 15 minutes to make garlic Denature and flocculate the miscellaneous proteins in the garlic slice processing wastewater, cool to 30°C, and then filter through diatomaceous earth hanging film to remove the miscellaneous proteins in the garlic slice processing wastewater; then filter and clarify the garlic slice processing wastewater, and pump it into A resin column equipped with 1,000 kg of superoxide dismutase (SOD...

Embodiment 3

[0033] With multiple 400-mesh stainless steel screens as the supporting material and 200-mesh diatomite as the filter medium, the diatomite feed liquid is circulated through the pump, and an 8 mm thick diatomite filter layer is evenly coated on the stainless steel screen in advance. Then 10 cubic meters of garlic slice processing wastewater are filtered to remove large particles of impurities and colloidal substances in the garlic slice processing wastewater; the filtered garlic slice processing wastewater is poured into the reactor, heated to 70°C, and the heating time is 18 minutes to make garlic Denature and flocculate the miscellaneous proteins in the garlic slice processing wastewater, cool to 30°C, and then filter through diatomaceous earth hanging film to remove the miscellaneous proteins in the garlic slice processing wastewater; then filter and clarify the garlic slice processing wastewater, and pump it into A resin column equipped with 1,000 kg of superoxide dismutase...

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PUM

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Abstract

The invention discloses a method for extracting garlic superoxide dismutase from garlic sheet processing waste water. The method comprises steps of diatomite membrane filtration, removal of impurity proteins by heating, superoxide dismutase molecular imprinting resin adsorption, eluent condensation and freeze drying, so as to obtain a garlic superoxide dismutase dry powder. According to the method for extracting garlic superoxide dismutase from garlic sheet processing waste water provided by the invention, the extracted garlic superoxide dismutase has high purity, low production costs, so that garlic processing waste water can discharged with standard reached, besides the garlic processing waste water resource is utilized effectively to extract valuable resources in waste water.

Description

technical field [0001] The invention relates to the field of resource recovery, development and utilization of agricultural product processing waste, in particular to a method for extracting garlic superoxide dismutase from garlic chip processing wastewater. Background technique [0002] Garlic (Allium sativum L) is a plant of the genus Allium in the Liliaceae family. It can be used for both medicine and food. It has the functions of antibacterial, anti-inflammatory, insecticidal, preventing atherosclerosis, lowering blood pressure, anti-rheumatism, preventing and treating tumors, and regulating the body's immunity. The chemical components of garlic are mainly divided into thiosulfinate esters, S-alk(ene)-L-cysteine ​​derivatives, sexual components, γ-L-glutamic acid polypeptides, glycosides, polysaccharides, lipids , enzymes, saponins. At present, the development and utilization of garlic is one of the hot spots of domestic and foreign medical research. [0003] At presen...

Claims

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Application Information

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IPC IPC(8): C12N9/08
Inventor 郑永军陈华郑康李延团
Owner SHANDONG SHUNBANG BIOTECH