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Antibody composition and application thereof

An antibody composition and antibody technology, applied in biological testing, biological material analysis, material inspection products, etc., can solve traumatic problems and achieve the effect of overcoming low sensitivity and specificity

Active Publication Date: 2011-10-19
THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current diagnosis of acute rejection and identification of rejection type (cellular versus antibody-mediated) relies solely on renal biopsy, which has the disadvantages of being limited and invasive

Method used

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  • Antibody composition and application thereof
  • Antibody composition and application thereof
  • Antibody composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Fractalkine, Mig, Granzyme B, and C4d were used alone and in combination to diagnose acute rejection of transplanted kidney

[0036] experimental method:

[0037] 1. Each ELISA kit was purchased from the company and the article number: Mig (R&D Systems, DCX900), Fractalkine (R&D Systems, DY365), Granzyme B (Bender MedSystems, BMS202), C4d (QUIDEL, A008)

[0038] 2. The research objects of this experiment are: 167 cases of non-rejection, 81 cases of acute rejection urine samples, urine samples are all from the transplant specimen bank of the First Affiliated Hospital of Zhejiang University School of Medicine Kidney Disease Center. The principle of urine specimen collection: morning urine was routinely collected at 2, 4, 6, 8 weeks after transplantation and postoperative follow-up; all transplanted kidney biopsies were collected immediately before biopsy. The collected urine samples should be stored in a -80°C freezer as soon as possible.

[0039] 3. ELISA as...

Embodiment 2

[0050] Example 2: Combining Fractalkine, Mig, C4d and BLC4 cytokines to diagnose acute rejection of transplanted kidney and identify rejection types

[0051] experimental method:

[0052] 1. Each ELISA kit was purchased from the company and the article number: Mig (R&D Systems, DCX900), Fractalkine (R&D Systems, DY365), C4d (QUIDEL, A008), BLC (R&D Systems, DCX130)

[0053] 2. The research objects of this experiment are: 167 cases of non-rejection, 81 cases of acute rejection urine samples, including 28 cases of antibody-mediated rejection, 53 cases of T cell-mediated rejection, all urine samples were from the Affiliated No. The transplant specimen bank of the nephrology center of a hospital. The principle of urine specimen collection: morning urine was routinely collected at 2, 4, 6, 8 weeks after transplantation and postoperative follow-up; all transplanted kidney biopsies were collected immediately before biopsy. The collected urine samples should be stored in a -80°C fre...

Embodiment 3

[0060] Example 3: Combining 5 cytokines, Fractalkine, Mig, Granzyme B, C4d and BLC, to diagnose acute rejection of transplanted kidney and to identify rejection types

[0061] experimental method:

[0062] 1. Each ELISA kit was purchased from the company and the article number: Mig (R&D Systems, DCX900), Fractalkine (R&D Systems, DY365), Granzyme B (Bender MedSystems, BMS202), C4d (QUIDEL, A008), BLC (R&D Systems, DCX130)

[0063] 2. The research objects of this experiment are: 167 cases of non-rejection, 81 cases of acute rejection urine samples, including 28 cases of antibody-mediated rejection, 53 cases of T cell-mediated rejection, all urine samples were from the Affiliated No. The transplant specimen bank of the nephrology center of a hospital. The principle of urine specimen collection: morning urine was routinely collected at 2, 4, 6, 8 weeks after transplantation and postoperative follow-up; all transplanted kidney biopsies were collected immediately before biopsy. T...

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Abstract

The invention belongs to the field of biotechnology and discloses an antibody composition and application thereof. The anti-body composition comprises a BLC specific antibody or comprises a Fractalkine specific antibody, a Mig specific antibody and a C4d specific antibody. The antibody composition can be used for diagnosing acute rejection after kidney transplant at one time and can be used for accurately diagnosing that the acute rejection belongs to the type of cellular rejection or antibody-mediated rejection. A detection reagent prepared by using the antibody composition overcomes the defect that current acute rejection diagnosis is only limited on renal biopsy and provides a noninvasive method for clinically diagnosing acute rejection of kidney transplant and acute rejection types ofkidney transplant.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to an antibody composition and its application, in particular to an antibody composition which can be used to prepare reagents for the diagnosis and differential diagnosis of acute rejection of kidney transplantation and its application. Background technique [0002] Kidney transplantation is the ideal renal replacement therapy for end-stage renal disease. With the advancement of transplant surgery technology and the application of new immunosuppressants, the survival rate of both human and kidney after kidney transplantation has been greatly improved, but the rejection reaction after kidney transplantation is still the main factor affecting the prognosis of kidney transplantation. And it is significantly related to the long-term survival rate of transplanted kidneys and transplant recipients. [0003] The diagnosis of rejection mainly depends on pathological biopsy of renal tissue. Howev...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/577
CPCG01N33/6893G01N2800/245
Inventor 陈江华彭文翰茅幼英寿张飞姜虹陈大进
Owner THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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