Method for determining content of fruit juice in orange type drinks based on principal component analysis
A principal component analysis and fruit juice content technology, applied in the analysis of materials, measuring devices, material separation, etc., can solve the problems of practicality and popularization, cumbersome operation process, high proficiency requirements, etc., and achieve accurate and reliable results
Inactive Publication Date: 2013-04-17
TONGJI UNIV
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However, in the national standard, the determination of the total flavonoid content is determined by spectrophotometry after adding a chromogenic agent. The operation process of the method is cumbersome, and the operator's proficiency is highly required, and the practicability and promotion degree are limited.
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Embodiment 1
[0041] 1. Obtaining LC chromatograms of standard samples and unknown samples
[0042] 1.1 Sample pretreatment
[0043] a. Use a measuring cylinder to measure 10.0 mL of 10 commercially available fruit juice products and 2 unknown fruit juice products (see Table 2 for the brands and serial numbers of various fruit juice products);
[0044] b. Add 10.0mL of methanol, ultrasonically assisted extraction for 15 minutes, filter, and dilute the filtrate to 25mL with methanol;
[0045] c. After filtering through an organic phase microporous membrane (0.45 μm), store in a cool place for testing.
[0046] Table 2 Fruit juice beverage experimental sample number
[0047] serial number brand name Packing Specifications Nominal Juice Content J1 Huiyuan Orange juice 1L 100% J2 farmer orchard orange juice drink 380mL 100% J3 Dole Orange juice 250mL 100% J4 great lake fresh orange juice 255mL 100% J5 holding hands orange ju...
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Abstract
The invention relates to a method for determining content of fruit juice in orange type drinks based on principal component analysis, which determines the content of the fruit juice in the orange type drinks through a liquid chromatogram separation technology by means of chemometrics methods. The method comprises the following steps of: processing a sample of the orange type drinks with ultrasonic extraction and the like, acquiring chromatographic data of the sample of the orange type drinks through an HPLC (high performance liquid chromatography)-DAD (diode array detection) technology, performing principal component analysis after preprocessing the acquired chromatographic data, and calculating out the content of the fruit juice in the orange type drinks. With the method provided by the invention, the content of the fruit juice of unknown orange type drinks can be determined, and the determination result is accurate and reliable.
Description
technical field [0001] The invention relates to a method for determining the juice content of orange beverages based on principal component analysis. Background technique [0002] At present, there is no uniform method for the determination of the content of orange juice in the world. Developed countries have their own methods for determining the content of fruit juice according to their national conditions and differences in fruit resource varieties. For example, in Japan and Taiwan, the fruit juice content is calculated by measuring the content of insoluble solids, total acid, amino acid nitrogen, ash, and soluble solids; Australia measures glucose, fructose, sucrose, potassium, phosphorus, calcium, ash and their The ratio between them is used to determine the content of fruit juice; the Netherlands, Spain, Israel, etc. refer to the relevant parameters in the RSK value of the Federal Republic of Germany, and formulate their own national standard values to determine the c...
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IPC IPC(8): G01N30/89G01N30/06
Inventor 沈梦洁冯昕韡朱仲良
Owner TONGJI UNIV