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Method for removing lignin by combined treatment of straws and laccase

A delignification and pretreatment technology, which is applied in the field of straw laccase combined pretreatment to remove lignin, can solve the problems of low accessibility of enzymes and raw materials, large enzyme amount, etc., and improve the moistening ability and removal rate High, increase the effect of adsorption performance and specific surface area

Inactive Publication Date: 2011-11-09
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] [Purpose of the present invention] Aiming at the problems that the lignin content of the physical steam explosion pretreatment method of lignocellulosic raw materials such as straw is still high, while the single enzymatic pretreatment uses a large amount of enzyme and the accessibility of enzyme and raw materials is low, the present invention Combining the mild and specific characteristics of enzyme treatment with the rapid and efficient characteristics of physical methods, the invention provides a method for combined pretreatment of straw laccase for delignification, in order to achieve efficient pretreatment of straw and provide cellulose and hemicellulose in straw Laying the foundation for effective clean separation and directional high-value conversion between lignin and lignin

Method used

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  • Method for removing lignin by combined treatment of straws and laccase
  • Method for removing lignin by combined treatment of straws and laccase
  • Method for removing lignin by combined treatment of straws and laccase

Examples

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Effect test

Embodiment 1

[0030]Corn stalks (13.42% moisture, 33.24% cellulose, 26.43% hemicellulose, 14.58% lignin, 7.24% ash) were cut into 10 mm long blocks, placed in a steam explosion device, and maintained at 1.5 MPa for 5 minutes. The pressure is suddenly released, and the obtained steam explosion material is separated from the solid and liquid by a screw extruder to obtain the washing liquid and the solid residue respectively. Dry in an oven at 60°C to obtain steam-exploded straw. The contents of cellulose, hemicellulose and lignin in steam-exploded straw were determined to be 45.28%, 10.21% and 18.43%, respectively. Take 20g of this steam-exploded straw, after crushing, add the basidiomycetes MyceliaSterilia laccase solution according to the laccase concentration of 0.55U / g substrate, adjust the pH of the reaction solution to 6.5, then add water to adjust the solid-liquid ratio to 1:20, mix well and place React in a water bath at 30°C for 4 hours, filter after the reaction, and weigh 17.6 g o...

Embodiment 2

[0032] Corn stalks (13.42% moisture, 33.24% cellulose, 26.43% hemicellulose, 14.58% lignin, 7.24% ash) were cut into 20 mm long blocks, placed in a steam explosion device, and the pressure was maintained at 2.0 MPa for 4 min. The pressure is suddenly released, and the obtained steam explosion material is separated from the solid and liquid by a screw extruder to obtain the washing liquid and the solid residue respectively. Place in an oven at 60°C to dry to obtain steam-exploded straw. The contents of cellulose, hemicellulose and lignin in the steam-exploded straw were determined to be 48.41%, 7.04% and 19.17% respectively; 20 g of the steam-exploded straw was taken, crushed According to the laccase concentration of 2.0U / g substrate, add versicolor versicolor laccase solution, adjust the pH of the reaction solution to 4.5, then add water to adjust the solid-liquid ratio to 1:20, mix well and place it in a water bath at 30°C for 6 hours. After the reaction was completed, the fi...

Embodiment 3

[0034] Wheat straw (12.62% moisture, 32.16% cellulose, 25.63% hemicellulose, 10.36% lignin, 6.52% ash) was cut into 50 mm lengths, placed in a steam explosion device, maintained at 1.2 MPa for 6 minutes, and suddenly released The obtained steam explosion material is separated from solid and liquid by a screw extruder to obtain washing liquid and solid residue respectively. After the solid residue is soaked in water according to the solid-liquid ratio of 1:2, it is extruded for the second time. The solid residue is placed in Dry in an oven at 60°C to obtain steam-exploded straw. The contents of cellulose, hemicellulose and lignin in the steam-exploded straw were determined to be 40.38%, 9.12% and 9.72%, respectively. Take 20g of this steam-exploded straw, after pulverizing, add the basidiomycete Mycelia Sterilia laccase solution according to the laccase concentration of 1.0U / g substrate, adjust the pH of the reaction solution to 8.0, then add water to adjust the solid-liquid rat...

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Abstract

The invention discloses a method for removing lignin by combined treatment of straws and laccase, belonging to technical field of pretreatment of biomass materials of lignocellulose. The method is characterized in that lignin in raw materials is removed by combination of steam-explosion physical pretreatment of straws and pretreatment of laccase so that the pretreatment effect is improved. In the invention, by means of the steam explosion action, the wet expansion capacity and the accessibility of the cellulose in straw raw materials are increased and the using amount of the enzyme for the subsequent pretreatment of steam-exploded straws and the laccase; and the laccase can be used for degrading the lignin selectively, such that the damaging action caused by the traditional biological method on the cellulose can be avoided and the period that the lignin is degraded by the laccase can be shortened. According to the invention disclosed by the invention, in the process of removing lignin by combined pretreatment of straws and laccase, alkali or organic solvents and the like are unnecessarily used, so that the method has the advantages of no pollution, high efficiency and the like.

Description

technical field [0001] The invention belongs to the technical field of pretreatment of lignocellulosic biomass raw materials, and in particular relates to a method for removing lignin by combining straw laccase pretreatment. Background technique [0002] Non-renewable fossil resources such as oil and natural gas form the cornerstone of the world's fuel and chemical industries today. However, since the 21st century, due to the non-renewability of fossil fuels and environmental problems such as the greenhouse effect, acid rain, and dust pollution caused by the use of fossil fuels, the production of high value-added chemical products from renewable plant resources, especially woody biomass, has become a major issue for many It is an important development field of the country and a hotspot of scientific research. Lignocellulosic biomass exists in large quantities in nature, and is the most abundant and cheapest renewable resource that meets the requirements of sustainable devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D21B1/02
Inventor 陈洪章邱卫华
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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