Automatic bacterial sorting and labeling method based on immune method, and apparatus thereof

Abstract

The invention discloses an automatic bacterial sorting and labeling method based on an immune method. The method comprises the following steps: 1) coating antibody molecules through magnetic beads; 2) coating the antibody molecules through a fluorescent material; 3) adding a sample solution; 4) conjugating the immunomagnetic beads and target bacterial; 5) carrying out magnetic separation; 6) carrying out washing; 7) labeling the target bacterial through the fluorescent material. The invention further discloses an apparatus for realizing the method. According to the present invention, simultaneous sorting and labeling of a plurality of the target bacterial can be realized so as to improve treatment efficiency; compared to the manual sorting and labeling method, liquid flow in the pipe is pushed through accurate actions of a injector pump provided by the apparatus, such that accurate control for the liquid flow can be realized, error due to a manual operation is avoided, the method has high repeatability.

Description

technical field [0001] The invention belongs to the technical field of biological sample sorting, in particular to the technical field of bacterial sorting based on an immune method, and specifically relates to an automatic bacterial sorting and marking method and device based on an immune method. Background technique [0002] With the continuous development of life sciences, the basic research of bacterial biology and immunology, as well as the diagnosis and treatment of clinical diseases require the isolation and purification of bacteria from mixed samples containing different bacteria. Existing bacterial separation techniques mainly include Ficoll density gradient centrifugation, centrifugal elution, two-phase separation, etc. These methods are based on the characteristics of bacterial size, density, surface charge and other physical properties to separate, and it is difficult to obtain ideal purity and Recovery rate. Cell sorting based on magnetic beads is a cell separa...

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Problems solved by technology

The culture method is a classic detection method with low cost and high sensitivity, but it takes a long time and does not meet the requirements of on-site rapid detection; the gold standard method is fast in detection, but it can only be used for qualitative detection, with low sensitivity and interference. There are many factors; enzyme-linked immunosorbent assay (ELISA) has high sensitivity, but there are many interfering factors affecting the results, and professional operation is required, and some positive judgments need to be based on statistics, which is relatively subjective; latex agglutination The method has fast detection speed and high sensitivity, but requires high reagent storage and is easy to agglutinate; while the particle detection method based on the principle of flow cytometry has the advantages of fast detection speed, high sensitivity and accuracy, so it has been more and more widely used.

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