Preparation method of metal ion coordination thin-layer boron nanosheet carrier probe with targeting, drug release and detection functions

Abstract

The invention discloses a preparation method of a metal ion coordination thin-layer boron nanosheet carrier probe with targeting, drug release and detection functions. A metal ion-boron atom coordination strategy is adopted to prepare a novel metal ion coordination thin-layer boron nanosheet (M@B-nanosheet); a conjugate (TPP-DOX) is prepared by adopting a carboxyl-amine coupling reaction and is loaded on the lamellar structure of the M@B-nanosheet to prepare a TPP-DOX / M@B-nanosheet compound; the compound is taken as a nano-carrier probe and enters cancer cells through endocytosis, conducts targeted transport and cell labeling in the cancer cells, and realizes targeted transport at a subcellular level is achieved; and controllable release of loaded drugs is executed under the change of a special microenvironment of a target point, and in-situ synchronous detection of drug release is executed by adopting fluorescence color difference change, so integration of triple functions of the carrier probe is obtained.

Description

technical field

[0001] The invention belongs to the technical field of preparation of functionalized nano-sheet composite materials and biomedical nano-carrier probes, and specifically relates to a method for preparing metal ion-coordinated thin-layer boron nano-sheet carrier probes with functions of targeting, drug release and detection . Background technique

[0002] Boron is the fifth element in the periodic table. Beryllium is adjacent to the metal on the left and non-metallic carbon is adjacent to the right. It has both metallic and non-metallic properties. It can form conventional strong covalent bonds and multi-center chemical bonds. The bonding ability and Way better than carbon. Among the more than one hundred crystal structures of boron, most of the bulk crystal boron contains B 12 Icosahedral structural units, a few dumbbell-shaped B containing γ-orthorhombic boron 2 unit. Boron forms abundant stable compounds with most elements, showing broad application pros...

Images