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Preparation method of metal ion coordination thin-layer boron nanosheet carrier probe with targeting, drug release and detection functions

A technology of metal ions and nanosheets, applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, nanotechnology for materials and surface science, etc., can solve the problem of metal ion coordination thin layer Boron nanosheet carrier probe and other issues

Active Publication Date: 2020-12-01
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no domestic and foreign literature and patent reports on the preparation and application of metal ion-coordinated thin-layer boron nanosheet carrier probes.

Method used

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  • Preparation method of metal ion coordination thin-layer boron nanosheet carrier probe with targeting, drug release and detection functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The schematic diagram of the preparation method and working principle of a metal ion-coordinated thin-layer boron nanosheet carrier probe with targeting, drug release and detection functions involved in this example is as follows figure 1 As shown, the specific preparation steps are as follows:

[0017] Under magnetic stirring, add 100 mg of boron powder and double-distilled aqueous solution in which 2 mg of magnesium nitrate was dissolved in sequence to 50 mL of isopropanol, mix well, transfer to an ultrasonic cell pulverizer, use a probe for ultrasonic treatment for 5 min, and then transfer to ultrasonic cleaning Ultrasonic treatment in a water bath for 12 hours in the device, the reaction product was centrifuged at 5000rpm for 5min, the upper layer solution was centrifuged at 10000rpm for 5min, the precipitate after centrifugation was washed three times with ethanol and double distilled water, and vacuum-dried to obtain the final product. Mg@B-nanosheets;

[0018] W...

Embodiment 2

[0022] A schematic diagram of the preparation method and principle of a metal ion-coordinated thin-layer boron nanosheet carrier probe with targeting, drug release and detection functions involved in this example is shown in figure 1 As shown, the preparation of the conjugate TPP-DOX is the same as in Example 1, and other specific preparation steps are as follows:

[0023] Under magnetic stirring, add 150 mg of boron powder and double-distilled aqueous solution in which 5 mg of calcium nitrate was dissolved into 75 mL of isopropanol in sequence, mix evenly, transfer to an ultrasonic cell pulverizer, use a probe for ultrasonic treatment for 10 min, and then transfer to ultrasonic cleaning Ultrasonic treatment in a water bath for 18 hours in the device, the reaction product was centrifuged at 5000rpm for 10min, the upper layer solution was centrifuged at 10000rpm for 10min, the precipitate after centrifugation was washed three times with ethanol and double distilled water, and va...

Embodiment 3

[0027] A schematic diagram of the preparation method and principle of a metal ion-coordinated thin-layer boron nanosheet carrier probe with targeting, drug release and detection functions involved in this example is shown in figure 1 As shown, the preparation of the conjugate TPP-DOX is the same as in Example 1, and other specific preparation steps are as follows:

[0028] Under magnetic stirring, add 200mg of boron powder and double-distilled aqueous solution in which 5mg of lithium nitrate was dissolved into 100mL of isopropanol successively, mix well, transfer to an ultrasonic cell pulverizer, use a probe to sonicate for 15min, and then transfer to an ultrasonic cleaner Ultrasonic treatment in a water bath for 24 hours, the reaction product was centrifuged at 5000rpm for 15min, the upper layer solution was centrifuged at 10000rpm for 15min, the precipitate after centrifugation was washed three times with ethanol and double distilled water, and vacuum dried to obtain the fina...

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Abstract

The invention discloses a preparation method of a metal ion coordination thin-layer boron nanosheet carrier probe with targeting, drug release and detection functions. A metal ion-boron atom coordination strategy is adopted to prepare a novel metal ion coordination thin-layer boron nanosheet (M@B-nanosheet); a conjugate (TPP-DOX) is prepared by adopting a carboxyl-amine coupling reaction and is loaded on the lamellar structure of the M@B-nanosheet to prepare a TPP-DOX / M@B-nanosheet compound; the compound is taken as a nano-carrier probe and enters cancer cells through endocytosis, conducts targeted transport and cell labeling in the cancer cells, and realizes targeted transport at a subcellular level is achieved; and controllable release of loaded drugs is executed under the change of a special microenvironment of a target point, and in-situ synchronous detection of drug release is executed by adopting fluorescence color difference change, so integration of triple functions of the carrier probe is obtained.

Description

technical field [0001] The invention belongs to the technical field of preparation of functionalized nano-sheet composite materials and biomedical nano-carrier probes, and specifically relates to a method for preparing metal ion-coordinated thin-layer boron nano-sheet carrier probes with functions of targeting, drug release and detection . Background technique [0002] Boron is the fifth element in the periodic table. Beryllium is adjacent to the metal on the left and non-metallic carbon is adjacent to the right. It has both metallic and non-metallic properties. It can form conventional strong covalent bonds and multi-center chemical bonds. The bonding ability and Way better than carbon. Among the more than one hundred crystal structures of boron, most of the bulk crystal boron contains B 12 Icosahedral structural units, a few dumbbell-shaped B containing γ-orthorhombic boron 2 unit. Boron forms abundant stable compounds with most elements, showing broad application pros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/63C07H15/252C09K11/06B82Y20/00B82Y30/00B82Y40/00A61K47/69A61K47/54A61K31/704A61P35/00G01N21/64
CPCC09K11/02C09K11/63C07H15/252C09K11/06B82Y20/00B82Y30/00B82Y40/00A61K47/6923A61K47/548A61K31/704A61P35/00G01N21/6402G01N21/6428G01N21/6458G01N21/6486
Inventor 桂日军孙玉娇孙泽君金辉
Owner QINGDAO UNIV
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