A kind of royal jelly polypeptide and its application

A technology for royal jelly and queen bee larvae, applied in the field of biomedicine, can solve the problems of few polypeptides, unsuitable for proteolysis of royal jelly, difficult to screen biologically active polypeptides, etc., and achieve the effect of inhibiting toxic effects

Inactive Publication Date: 2011-11-30
BEIJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there have been few studies on the enzymatic hydrolysis of royal jelly polypeptides, and most of the enzymatic hydrolysis methods are carried out by exogenous enzymes such as pepsin, trypsi

Method used

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  • A kind of royal jelly polypeptide and its application
  • A kind of royal jelly polypeptide and its application
  • A kind of royal jelly polypeptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. After adding 50mmol / L phosphate buffer with a pH value of 7 and diluting with the mass volume ratio (g / mL) of the intestinal tract of the worm body (g / mL) at 1:1, grind it into a homogenate with an electric homogenizer under ice-bath conditions. Centrifuge at 20,000×g for 20 min at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of suspended solution, the lower layer of sedimentation, and the middle layer is recovered. The middle layer was centrifuged again at 20000×g at 4°C for 20 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.

[0059] Take part of the intestinal enzyme solution, and use the BCA method to de...

Embodiment 2

[0064] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. After adding 50mmol / L phosphate buffer with a pH value of 7 and diluting with the mass volume ratio (g / mL) of the intestinal tract of the worm body (g / mL) at 1:1, grind it into a homogenate with an electric homogenizer under ice-bath conditions. Centrifuge at 18000×g for 30 min at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of suspended solution, the lower layer of sedimentation, and the middle layer is recovered. The middle layer was centrifuged again at 18000×g at 4°C for 30 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.

[0065] Take part of the intestinal enzyme solution, and use the BCA method to d...

Embodiment 3

[0070] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. After adding 50mmol / L phosphate buffer with a pH value of 7.2 and diluting with the mass volume ratio (g / mL) of the intestinal tract of the worm body (g / mL) being 1:1.5, it was ground into a homogenate with an electric homogenizer under ice bath conditions. Centrifuge at 25,000×g for 24 minutes at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of the suspended solution, the lower layer of sedimentation, and the recovery of the middle layer. The middle layer was centrifuged again at 25000×g at 4°C for 24 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.

[0071] Take part of the intestinal enzyme solution, and...

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Abstract

The invention relates to the technical field of biomedicine, in particular relates to a royal jelly polypeptide and application thereof. According to the invention, intestinal tract enzyme is extracted from a queen bee larva in vivo, and an in vivo enzymolysis environment of queen bee is simulated to carry out enzymolysis on royal jelly so as to obtain 1-3kDa of royal jelly polypeptide. The royal jelly polypeptide can be used for preparing an A beta inhibitor.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a royal jelly polypeptide and its application. Background technique [0002] In recent years, with the increase of the average life expectancy of human beings, the proportion of the elderly population has been increasing, and the aging of my country's population is becoming more and more obvious. Alzheimer's disease (AD), as the most common type of senile dementia, has caused the whole society to extensive attention. Alzheimer's disease is a complex multifactorial chronic progressive mental decline disease characterized by memory loss and cognitive dysfunction, clinical manifestations are progressive memory and cognitive decline, language barriers, psychomotor abnormalities and other symptoms . In the early stage of the disease, the near memory is mainly decreased, and the distant memory is also affected in the later stage of the disease, and the daily life is affected. , d...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K2/00C07K1/34A61K38/01A61P25/28
Inventor 冯成强孙平颜慧李虎臣
Owner BEIJING NORMAL UNIVERSITY
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