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A low-temperature degrading polycyclic aromatic hydrocarbon strain and its application in groundwater bioremediation of petroleum hydrocarbon-contaminated sites

A polycyclic aromatic hydrocarbon and bioremediation technology, which is applied in the fields of environmental engineering and microbial engineering, can solve the problems of refractory engineering of polycyclic aromatic hydrocarbon degrading bacteria and many types of polycyclic aromatic hydrocarbons, and achieves a wide range of degradation substrates, broad application potential, The effect of rapid degradation

Inactive Publication Date: 2011-12-14
LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY
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Problems solved by technology

However, the ambient temperature of groundwater in actual PAH-contaminated sites is low (10-15°C), and the types of PAHs are relatively low (10μg / L-20mg / L), which makes the screened PAH-degrading bacteria very difficult. Difficult to apply in actual groundwater bioremediation projects, in which temperature influence is the most prominent

Method used

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  • A low-temperature degrading polycyclic aromatic hydrocarbon strain and its application in groundwater bioremediation of petroleum hydrocarbon-contaminated sites

Examples

Experimental program
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Embodiment 1

[0037] The isolation and screening method of Neosphingobacterium provided by the invention.

[0038] In winter in the north, when the outdoor temperature is less than -20°C for a long period of time, surface soil samples at a distance of 5-20 cm below the surface and groundwater samples are collected from a contaminated site in Jilin Oilfield, and they are used as bacterial sources for low-temperature natural enrichment culture. The soil and groundwater samples taken are about to be made into bacterial suspension, and the supernatant is added to the enrichment medium [inorganic salt medium (K 2 HPO 4 1550, NaH 2 PO 4 850, (NH 4 ) 2 SO 4 2000, MgCl 2 ·6H 2 O 100, EDTA 10, ZnSO 4 ·7H 2 O 2.0, CaCl 2 ·2H 2 O 1.0, FeSO 4 ·7H 2 O 5.0, NaMoO 4 ·2H 2 O 0.2, CuSO 4 ·5H 2 O 0.2, CoCl 2 ·6H 2 O 0.4, MnCl 2 ·2H 2 O 1.0, pH 7.0, unit: mg / L), 0# diesel oil 10 mL / L, glucose 5 g / L, yea...

Embodiment 2

[0042] Low-temperature degradation of polycyclic aromatic hydrocarbons by growth cells of Sphingobacterium neoformans.

[0043] Inoculate Sphingobacterium neoformans into polluted groundwater containing 300 mg / L diesel oil, and at the same time make a blank control without inoculation, place the above samples at 10°C and culture on a shaker at 150 rpm for 3 days, and perform full-scan analysis by gas chromatography-mass spectrometry after extraction , compared with the spectrum library, neosphingobacterium can completely degrade polycyclic aromatic hydrocarbons in diesel oil: naphthalene, 1-methylnaphthalene, 2-methylnaphthalene, 2-ethylnaphthalene, 2,6-dimethylnaphthalene Naphthalene, 2,7-dimethylnaphthalene, 1,8-dimethylnaphthalene, 1,4-dimethylnaphthalene, 1,6-dimethylnaphthalene, 1,4,5-trimethylnaphthalene, 1 , 6,7-trimethylnaphthalene, 2,3,6-trimethylnaphthalene, 2-methylbiphenyl, 4-methylbiphenyl, phenanthrene, 1-methylphenanthrene, 2-methylphenanthrene, 4 -Methylph...

Embodiment 3

[0045] Low-temperature degradation of polycyclic aromatic hydrocarbons by static-based cells of Sphingobacterium neoformans.

[0046] Inoculate neosphingobacterium in diesel inorganic salt medium (the medium is the same as above, the concentration of diesel oil is 300mg / L), 10 ℃, 150rpm shaker culture to the logarithmic growth phase, the number of bacteria is about 10 9 -10 11 cells / mL, the strain suspension was centrifuged at 8000rpm at 4°C for 15 minutes at high speed, washed with phosphate buffer, and then centrifuged and washed three times, and finally the bacteria were collected to prepare a static-based cell suspension. Liquid (the number of bacteria is about 10 10 individual / mL). Carry out the following experiments respectively.

[0047] (1) Inoculate the static-based cell suspension of Sphingobacterium neosphingosus at 5% (V / V) in naphthalene-contaminated groundwater (the concentration of naphthalene is 20 mg / L). At a low temperature of 10°C, the de...

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Abstract

The invention belongs to the technical fields of environmental engineering and microbial engineering, and in particular relates to a neosphingobacterium strain that degrades polycyclic aromatic hydrocarbons at low temperature and its application in groundwater bioremediation of petroleum hydrocarbon polluted sites. The strain is Novosphingobium sp., which was deposited in the "General Microbiology Center of China Committee for the Collection of Microorganisms" on October 12, 2009, with the preservation number CGMCC No.3326. The bacterial strain of the present invention is isolated from a certain polluted site in Jilin Oilfield, and obtained through low-temperature enhanced natural enrichment and screening. It can degrade polycyclic aromatic hydrocarbon pollutants under low temperature conditions, and is especially suitable for bioremediation of groundwater in petroleum hydrocarbon-contaminated sites.

Description

technical field [0001] The invention belongs to the technical fields of environmental engineering and microbial engineering, and in particular relates to a neosphingobacterium that degrades polycyclic aromatic hydrocarbons at low temperature and its application in groundwater bioremediation of petroleum hydrocarbon polluted sites. Background technique [0002] Polycyclic aromatic hydrocarbon molecules contain more than two benzene ring structures, which are unsaturated hydrocarbons, mainly including: bicyclic aromatic hydrocarbons (naphthalene), tricyclic aromatic hydrocarbons (anthracene, phenanthrene) and aromatic hydrocarbons with more than three rings (such as benzopyrene, benzanthracene) ), among which two-ring and three-ring aromatics are the most common, which widely exist in crude oil and petroleum products (gasoline, kerosene, diesel, etc.). [0003] With the production and widespread use of petroleum and its products, the pollution of polycyclic aromatic hydrocarbo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34
Inventor 马会强张洪林张兰英李爽李长波赵国峥
Owner LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY
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