A seminal plasma α-glucosidase activity quantitative detection kit and its application

A quantitative detection, glycosidase technology, applied in the field of biomedical testing and determination, can solve the problem of no automatic biochemical instrument detection kit, etc., and achieve the effects of shortening detection time, simplifying operation steps, and reducing human errors

Inactive Publication Date: 2011-12-14
南京欣迪生物药业工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regrettably, as of now, no matter whether it is foreign or domestic, for the detection of seminal plasma α-glucosidase, except for the early scientific research results, there is still no commercialized detection that can be matched with an automatic biochemical analyzer Therefore, it is particularly urgent and important to establish a seminal plasma α-glucosidase activity quantitative detection kit and detection method for use with an automatic biochemical analyzer.

Method used

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  • A seminal plasma α-glucosidase activity quantitative detection kit and its application
  • A seminal plasma α-glucosidase activity quantitative detection kit and its application
  • A seminal plasma α-glucosidase activity quantitative detection kit and its application

Examples

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Effect test

Embodiment 1

[0050] A seminal plasma α-glucosidase activity quantitative detection kit in this example is a double reagent, including R1 reagent and R2 reagent, prepared according to the following components and dosage respectively:

[0051] 1) R1 reagent

[0052] pH7.0 phosphate buffer 100mmol / L;

[0053] Glucose oxidase 1.2U / L;

[0054] Peroxidase 1.2U / L;

[0055] Thimerosal 0.05%;

[0056] 2) R2 reagent:

[0057] pH7.0 phosphate buffer 100mmol / L;

[0058] Phenol 106mmol / L;

[0059] Maltose 87mmol / L;

[0060] 4-aminoantipyrine 0.4mmol / L;

[0061] Gentamicin 0.05%;

[0062] After the above reagents are completely dissolved, they are divided into bottles to make liquid double reagents, which can be used directly.

Embodiment 2

[0064] A seminal plasma α-glucosidase activity quantitative detection kit in this example is a double reagent, including R1 reagent and R2 reagent, prepared according to the following components and dosage respectively:

[0065] 1) R1 reagent

[0066] pH6.8 phosphate buffer 110mmol / L;

[0067] Glucose oxidase 2.0U / L;

[0068] Peroxidase 2.0U / L;

[0069] Gentamicin 0.1%;

[0070] 2) R2 reagent:

[0071] pH6.8 phosphate buffer 110mmol / L;

[0072] 3,5-dichloro-2-hydroxybenzenesulfonate sodium 100mmol / L;

[0073] Maltose 70mmol / L;

[0074] 4-aminoantipyrine 1.0mmol / L;

[0075] ProClin150 0.1%;

[0076] After the above reagents are completely dissolved, they are divided into bottles to make liquid double reagents, which can be used directly.

Embodiment 3

[0078] A seminal plasma α-glucosidase activity quantitative detection kit in this example is a double reagent, including R1 reagent and R2 reagent, prepared according to the following components and dosage respectively:

[0079] 1) R1 reagent

[0080] pH6.9 Tris buffer 90mmol / L;

[0081] Glucose oxidase 3.0U / L;

[0082] Peroxidase 2.5U / L;

[0083] ProClin300 0.01%;

[0084] 2) R2 reagent:

[0085] pH6.9 Tris buffer 90mmol / L;

[0086] 4-amino-3-methylbenzoic acid 115mmol / L;

[0087] Maltose 100mmol / L;

[0088] 4-aminoantipyrine 0.2mmol / L;

[0089] ProClin950 0.01%;

[0090] After the above reagents are completely dissolved, they are divided into bottles to make liquid double reagents, which can be used directly.

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Abstract

The invention discloses a seminal plasma α-glucosidase activity quantitative detection kit, which includes R1 reagent and R2 reagent; the R1 reagent includes the following components: 90-110mmol / L pH6.8-7.0 buffer solution, 1.0-3.0 U / L glucose oxidase, 1.0-2.5U / L peroxidase and 0.01-0.1% preservative; R2 reagent includes the following components: 90-110mmol / L pH6.8-7.0 buffer, 100-115mmol / L color former, 70-100mmol / L maltose, 0.2-1.0mmol / L 4-aminoantipyrine and 0.01-0.1% preservative. The invention also discloses the application of the seminal plasma alpha-glucosidase activity quantitative detection kit in measuring seminal plasma alpha-glucosidase activity. The present invention only uses two kinds of liquid reagents, and the types and dosage of reagents are greatly reduced; the rate method is used for detection, and the sample does not need to be pre-diluted, which shortens the reaction time; it is used in conjunction with a fully automatic biochemical instrument, which simplifies the steps and saves reagents. The human error is reduced, and the automatic and large-scale detection greatly improves the detection efficiency while improving the accuracy and reliability of the detection results.

Description

technical field [0001] The invention belongs to the technical field of biomedical testing and measurement, and relates to a kit for in vitro diagnosis and its application, in particular to a seminal plasma α-glucosidase activity quantitative detection kit and its application. Background technique [0002] The epididymis is the place where sperm are transported, matured, and stored. The decline of epididymis secretory function will have adverse effects on the activity and function of sperm. At present, the indicators used to detect the secretory function of the epididymis include glycerophosphocholine, L-carnitine, and α-glucosidase (α-glucosidase). Studies have shown that compared with L-carnitine and glycerophosphorylcholine, α-glucosidase is more specific and sensitive for the detection of epididymis secretory function in patients with vasectomy and confirmed genital tract obstruction, which is considered to be the guarantee of epididymis secretory function. Marker enzyme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34C12Q1/28C12Q1/26
Inventor 高建陆金春陈晓慧
Owner 南京欣迪生物药业工程有限责任公司
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