Method, kit and application for diagnosing thalassemia based on liquid chip system

Abstract

Disclosed are a method for diagnosing thalassemia based on a liquid chip system, and a kit and an application. The present invention designs probes for detecting a, ß thalassemia genetic defect types. These probes are respectively cross-linked and mixed with fluorescent encoding microspheres of different colors to obtain liquid chips for diagnosing a, ß thalassemia. After PCR amplification is performed, by using a specific primer of the present invention, on a sample to be detected, a biotin-labeled PCR product is obtained, which is then hybridized with the liquid chip of the present invention, and further undergoes fluorescence labeling by use of phycoerythrin. Finally, a detection result is read out through a liquid chip detector. The kit of the present invention comprises PCR primers and specific probes, and can detect deletion or non-deletion point mutation a, ß thalassemia.

Description

technical field [0001] The invention relates to a method for diagnosing thalassemia, in particular to a method, kit and application for diagnosing thalassemia based on a liquid phase chip system. Background technique [0002] Thalassemia (referred to as thalassemia) is the most common human monogenic hereditary blood disease in the world. It is a group of hereditary diseases caused by the loss or point mutation of the globin gene, which reduces or fails to synthesize the globin peptide chain in hemoglobin. Hemolytic anemia. The disease is mainly seen in the west from the Mediterranean coast, through Turkey, Middle East countries in the middle, east to Southeast Asian countries and southern my country. The provinces south of the Yangtze River in my country have the highest incidence rate, among which Guangdong, Guangxi, Sichuan, Yunnan, Hainan, Hong Kong and Taiwan are more common. According to the type of globin chain lacking in this disease and the degree of deficiency, i...

AI Technical Summary

Problems solved by technology

At present, there have been reports on the detection of thalassemia using liquid-phase chips, but the detection sites are few, the technology is not mature enough, and it cannot meet the clinical needs, and there are no similar products on the market at home and abroad.

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