Multiplex polymerase chain reaction amplification instrument
A chain reaction and polymerase technology, which is applied in bioreactor/fermenter combinations, specific-purpose bioreactors/fermenters, biochemical instruments, etc. Unfavorable detection work and other problems, to achieve the effect of shortening detection time, simple structure and convenient operation
Inactive Publication Date: 2013-01-02
WUHAN NEWORLD BIOTECH
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Problems solved by technology
However, some of the existing PCR instruments have a heating and cooling process, and the entire amplification time is relatively long, which is especially limited in the detection of sudden infectious diseases.
Some PCR instruments are complicated to operate (such as the placement and removal of the patent CN101748056 A sample chip is very inconvenient) which is not conducive to the operation of a large number of clinical detection work
Method used
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[0027] refer to figure 1 , the device mainly includes a casing 1 . A transmission device 28 is installed inside the casing 1, a wheel disc 16 is installed above the transmission device 28, a heating temperature control device 29 is installed between the wheel disc 16 and the transmission device 25, and the transmission device 28 drives the wheel disc 16 to move from one temperature zone to another. Another temperature zone. A touch display screen 2 is installed on the front end of the casing 1 , and a USB port 7 and a JTAG port 8 are installed on the rear end of the casing. A control board 3 is installed in the casing 1 , and the control board 3 controls the heating and temperature control device 29 , the touch screen 2 , the USB port 7 and the JTAG port 8 .
[0028] refer to Figure 4 , The transmission device 28 includes a fixed frame 22 , an eccentric wheel 23 and a limit cover plate 26 . The fixed frame 22 is installed in the transmission installation hole in the casin...
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The invention discloses a multiplex polymerase chain reaction amplification instrument, belonging to the technical field of polymerase chain reaction. The instrument comprises a case, wherein a transmission device is installed inside the case; a rotary table is installed above the transmission device; a heating temperature control device is installed between the rotary table and the transmission device; the transmission device drives the rotary table to move from a temperature area to another temperature area; a touch display screen is installed at the front end of the case; a USB (Universal Serial Bus) port and a JTAG (Joint Test Action Group) port are installed at the rear end of the case; a control board is installed inside the case; and the control board is used for controlling the heating temperature control device, the touch display screen, the USB port and the JTAG port. The instrument is convenient to operate, increases the amplification speed of a PCR (polymerase chain reaction) instrument, shortens the entire detection time, can be more conveniently applied to clinical detection, in particular to detection of emergent infectious diseases, and gains precious time for disease treatment and control.
Description
technical field [0001] The invention belongs to the technical field of polymerase chain reaction, in particular to a multiplex polymerase chain reaction amplification instrument. Background technique [0002] PCR technology (that is, polymerase chain reaction) is the expansion of specific genes according to the characteristics of DNA modules under the catalysis of DNA polymerase in microbial samples. The whole working process is completed by three cycles of high-temperature denaturation, low-temperature annealing and medium-temperature primer extension under constant temperature conditions at three different temperatures. A sample generally requires 20-40 such cycle reactions to achieve DNA amplification. [0003] Since the invention of the epoch-making polymerase chain reaction (Polymerase Chain Reaction, PCR), people can purposefully and infinitely amplify specific nucleic acid fragments in vitro. Its principle is similar to the replication of DNA in vivo, except that a ...
Claims
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IPC IPC(8): C12M1/00C12M1/38
Inventor 孔令学廖晓志D·苏格玛秦操程仁镇
Owner WUHAN NEWORLD BIOTECH