New cancer-resisting use of fritillaria cirrhosa total alkaloids and compounds contained in fritillaria cirrhosa total alkaloids
A technology of total alkaloids and Fritillaria chinensis, applied in the direction of medical preparations containing active ingredients, drug combinations, antineoplastic drugs, etc., can solve problems that cannot meet the needs of large-scale production and clinical practice, report of fritillary fritillary anticancer activity, extraction and separation Problems such as high purification cost, to achieve the effect of being convenient for large-scale production and clinical use, inhibiting the proliferation of eukaryotic tumor cells, and simple preparation methods
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[0046] Example 1 Extraction of total alkaloids from Fritillaria cirrhosa of the present invention
[0047] The crude powder of Fritillaria cirrhosa was extracted with six times the amount of methanol (organic solvents such as ethanol) for three consecutive reflux extractions for 2 hours each time, concentrated under reduced pressure, and vacuum dried at 45°C for 10 hours to obtain a crude extract. The crude extract is kneaded with an appropriate amount of 3% HCl (the so-called kneading is to disperse by hand to dissolve, and the effect of hand squeezing makes the sample become fine and fine particles to accelerate the dissolution) to obtain an acid solution, which is extracted three times with petroleum ether. , Adjust the pH value to 10 with ammonia water, extract four times with appropriate chloroform, and place it in a vacuum drying oven at 45°C for 72 hours to obtain the total alkaloid extract of Fritillaria cirrhosa (Total alkaloids Fritillaria I).
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[0048] Example 2 Extraction of total alkaloids from Fritillaria cirrhosa of the present invention
[0049] Take an appropriate amount of Fritillaria cirrhosa, crush it, sieving through a 60-mesh sieve, soak it with 10% ammonia water for 24 hours, and place it in a reflux extraction device with chloroform-methanol (4:1) reflux extraction for 3 times, each for 3 hours. Concentrate under reduced pressure and recover the extract to obtain a brown extract. The extract was kneaded with an appropriate amount of 3% HCl to obtain an acid solution. The acid solution was extracted three times with petroleum ether, adjusted to pH 10 with ammonia, extracted four times with chloroform, and dried in a vacuum drying cabinet at 45°C for 72 hours. Get the total alkaloids of Fritillaria cirrhosa (Total alkaloids of Fritillaria cirrhosa II). The amount of ammonia used in the infiltration of the present invention is based on the liquid level that can surpass the medicinal materials to be extracted. ...
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[0050] Example 3 Extraction of total alkaloids from Fritillaria cirrhosa of the present invention
[0051] Take an appropriate amount of Fritillaria cirrhosa, soak it in an appropriate amount of ammonia water for 2 hours, and place it in a reflux extraction device with chloroform-methanol (2:1) reflux extraction for 3 times, each for 3 hours. Concentrate under reduced pressure and recover the extract to obtain a brown extract. The extract was kneaded with an appropriate amount of 3% HCl to obtain an acid solution. The acid solution was extracted three times with petroleum ether, adjusted to pH 10 with ammonia, extracted four times with chloroform, and dried in a vacuum drying cabinet at 45°C for 72 hours. Get the total alkaloids of Fritillaria cirrhosa (Total alkaloids of Fritillaria cirrhosa III).
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