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New cancer-resisting use of fritillaria cirrhosa total alkaloids and compounds contained in fritillaria cirrhosa total alkaloids

A technology of total alkaloids and Fritillaria chinensis, applied in the direction of medical preparations containing active ingredients, drug combinations, antineoplastic drugs, etc., can solve problems that cannot meet the needs of large-scale production and clinical practice, report of fritillary fritillary anticancer activity, extraction and separation Problems such as high purification cost, to achieve the effect of being convenient for large-scale production and clinical use, inhibiting the proliferation of eukaryotic tumor cells, and simple preparation methods

Inactive Publication Date: 2012-01-25
SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low content of peiminin and peiminin in Fritillaria japonicus, the cost of extraction, separation and purification is high, which cannot meet the needs of large-scale production and clinical practice
[0005] At present, there is no anti-cancer activity on the total alkaloids of Fritillaria chinensis, Chuanbeicone, Sibetine, Sibetine nitrogen oxides, isobetrine, isobetrine nitrogen oxides, and fritillaria. Aspects of reports

Method used

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  • New cancer-resisting use of fritillaria cirrhosa total alkaloids and compounds contained in fritillaria cirrhosa total alkaloids
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  • New cancer-resisting use of fritillaria cirrhosa total alkaloids and compounds contained in fritillaria cirrhosa total alkaloids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 Extraction of total alkaloids of Fritillaria sichuanensis of the present invention

[0047] Take the coarse powder of Fritillaria chinensis, extract with six times the amount of methanol (ethanol and other organic solvents) under continuous reflux for three times, each time for 2 hours, concentrate under reduced pressure, and dry under vacuum at 45°C for 10 hours to obtain a crude extract. The crude extract is kneaded with an appropriate amount of 3% HCl (the so-called kneading is to disperse by hand until it is dissolved, and the effect of hand extrusion makes the sample become fine and tiny particles to accelerate the dissolution) to obtain an acid solution, which is extracted three times with an appropriate amount of petroleum ether , adjusted the pH value to 10 with ammonia water, extracted four times with chloroform, and dried in a vacuum oven at 45° C. for 72 hours to obtain the total alkaloid extract of Fritillaria chinensis (Total Alkaloids of Fritil...

Embodiment 2

[0048] Embodiment 2 Extraction of total alkaloids of Fritillaria sichuanensis of the present invention

[0049] Take an appropriate amount of Fritillaria chinensis, crush it, pass through a 60-mesh sieve, infiltrate it with 10% ammonia water for 24 hours, put it in a reflux extraction device and use chloroform-methanol (4:1) to reflux and extract it 3 times, each time for 3 hours. Concentrate under reduced pressure and recover the extract to obtain a brown extract. The extract was kneaded and dissolved with an appropriate amount of 3% HCl to obtain an acid solution, which was extracted three times with petroleum ether, adjusted to pH 10 with ammonia water, extracted four times with chloroform, and dried in a vacuum oven at 45°C for 72 hours. Total alkaloids of Fritillaria dechuan (Fritillary fritillary total alkaloids II). The amount of ammonia water used during the infiltration of the present invention is based on the fact that the liquid surface can cover the medicinal mate...

Embodiment 3

[0050] Embodiment 3 Extraction of total alkaloids of Fritillaria sichuanensis of the present invention

[0051] Take an appropriate amount of Fritillaria chinensis, infiltrate it with ammonia water for 2 hours, put it in a reflux extraction device and use chloroform-methanol (2:1) to reflux and extract 3 times, each time for 3 hours. Concentrate under reduced pressure and recover the extract to obtain a brown extract. The extract was kneaded and dissolved with an appropriate amount of 3% HCl to obtain an acid solution, which was extracted three times with petroleum ether, adjusted to pH 10 with ammonia water, extracted four times with chloroform, and dried in a vacuum oven at 45°C for 72 hours. Total alkaloids of Fritillaria dechuan (Fritillary fritillary total alkaloids III).

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Abstract

The invention provides use of fritillaria cirrhosa total alkaloids in preparing drugs for inhibiting multiplication of eukaryote tumor cells. The invention also provides new cancer-resisting use of compounds in fritillaria cirrhosa. The fritillaria cirrhosa total alkaloids can inhibit the multiplication of the eukaryote tumor cells, has an effect on curing tumors and can be used for curing multiple tumors. With the same dose, the drug effect of the fritillaria cirrhosa total alkaloids is obviously better than the compound peimine and peiminine. The fritillaria cirrhosa total alkaloids have stable quality, are safe to use and are easy to control, the preparation method is simple, and the mass production and clinical use can be conveniently realized. Monomeric compounds such as chuanbeinone, kashmirine, imperialine-beta-N-oxside, isoverticine, isoverticine-beta-N-oxide and peimisine contained in the fritillaria cirrhosa total alkaloids also can inhibit the multiplication of the eukaryote tumor cells and have an effect on curing the tumor; and with the same dose, the drug effect of the monomeric compounds in the fritillaria cirrhosa total alkaloids is obviously better than that of peimine and peiminine, and a new choice for clinically curing the tumor is provided.

Description

technical field [0001] The invention relates to a new anticancer application of alkaloid components contained in Fritillaria sichuanensis, and also relates to a total alkaloids of Fritillaria sichuanensis and a pharmaceutical composition containing the extract, belonging to the field of medicines. Background technique [0002] According to the statistics of the World Health Organization (WHO), 10 million people in the world suffer from cancer every year, and the number of people who die from cancer accounts for 12% of the global death toll. There are 1.8 million new cancer patients and 1.4 million deaths every year in our country, and the incidence of cancer in our country shows a sharp upward trend. In the past less than 20 years, the incidence of cancer in my country has increased by 69%, and the death rate has increased by 29.4%. Therefore, cancer is seriously threatening the health of the people of the world and the Chinese people. The treatment of tumors with traditio...

Claims

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Application Information

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IPC IPC(8): A61K36/9066A61K31/58A61P35/00
Inventor 王曙王冬冬冯莉王仁杰
Owner SICHUAN UNIV
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