Method for improving freeze-drying survival rate of Lactobacillus acidophilus
A technology of Lactobacillus acidophilus and survival rate, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low number of viable bacteria and low survival rate of freeze-drying
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Embodiment 1-1
[0019] Sterilize the MRS broth at 118°C for 15 minutes, cool it to room temperature, and then inoculate Lactobacillus acidophilus at 4% (V / V) inoculum, culture at 37°C for 18 hours, and then place the culture solution at 9°C for 1.0 h, take a sample to measure the number of viable bacteria, then centrifuge at 4°C, 7000 rpm for 10 min, discard the supernatant to obtain Lactobacillus acidophilus sludge, weigh the sludge, and add 20% (W / W) skim milk of the sludge weight Powder, then add 80% (W / W) phosphate buffer of pH 6.8 to the weight of bacteria mud, then pre-freeze at -40°C for 8 hours, and then place it in a freeze dryer for lyophilization, 4-6Pa, -50 Freeze-drying at ℃ for 24h, you can get Lactobacillus acidophilus powder, the freeze-drying survival rate is 70.45% (control group is 46.11%), the number of viable bacteria is 2.1×10 11 cfu / g((The control group is 1.10×10 11 cfu / g)).
Embodiment 1-2
[0021] Sterilize the MRS broth at 118°C for 15 minutes, cool it to room temperature, and then inoculate Lactobacillus acidophilus at a 4% (V / V) inoculum, culture at 37°C for 18 hours, and then place the culture solution at 8°C for 0.5 h, take a sample to measure the number of viable bacteria, then centrifuge at 4°C, 7000 rpm for 15 minutes, discard the supernatant to obtain Lactobacillus acidophilus sludge, weigh the sludge, and add 10% (W / W) weight of the sludge with skim milk Powder, then add 90% (W / W) phosphate buffer solution of pH 6.8 with the weight of bacteria mud, then pre-freeze at -40℃ for 12h, then put it in a freeze dryer for lyophilization, 6Pa, -60℃ Lyophilize for 21h to obtain Lactobacillus acidophilus powder.
Embodiment 1-3
[0023] Sterilize the MRS broth at 118°C for 15 minutes, cool it to room temperature, and then inoculate Lactobacillus acidophilus at a 4% (V / V) inoculum, culture at 37°C for 18 hours, and then place the culture solution at 10°C for 1.5 h, take a sample to measure the number of viable bacteria, then centrifuge at 4°C, 7000 rpm for 11 minutes, discard the supernatant to obtain Lactobacillus acidophilus sludge, weigh the sludge, and add 16% (W / W) skim milk of the sludge weight Powder, then add 84% (W / W) phosphate buffer solution of pH 6.8 with the weight of bacteria mud, then pre-freeze at -40℃ for 11h, then put it in a freeze dryer for lyophilization, 4Pa, -52℃ Lyophilize for 18h to obtain Lactobacillus acidophilus powder.
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