Flora construction method for residual oil gasification exploitation of oil deposit

A construction method and reservoir technology, applied in the direction of using microorganisms, chemical instruments and methods, bacteria, etc., to achieve the effect of overcoming bias and one-sidedness

Active Publication Date: 2012-01-25
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no information on how to establish the bacterial community and its construction method for the gasification of residual oil in the reservoir.

Method used

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  • Flora construction method for residual oil gasification exploitation of oil deposit
  • Flora construction method for residual oil gasification exploitation of oil deposit
  • Flora construction method for residual oil gasification exploitation of oil deposit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The sample comes from the fluid produced by an oil well in Huabei Oilfield, and the reservoir temperature is 38°C. 30 μL of mixed alkanes (n-pentadecane, n-hexadecane, n-heptadecane, n-octadecane, n-nonadecane and n-eicosane are uniformly mixed in equal volumes), vitamin solution 0.2mL (vitamin B121.0mg / L, biotin 20.0mg / L, folic acid 20.0mg / L, niacin 50.0mg / L, D-Ca-pantothenate 50.0mg / L, p-aminobenzoic acid 50.0mg / L, vitamin B6-hydrochloric acid 100.0mg / L, riboflavin 50.0mg / L, vitamin B1-hydrochloric acid (2H 2 (2) 50.0mg / L, lipoic acid 50.0mg / L) and trace element solution 0.1mL (CoCl 2 ·6H 2 O 0.50g / L, CuCl 2 2H 2 O 0.10g / L, FeCl 2 4H 2 O 7.50g / L, H 3 BO 3 1.00g / L, MnCl 2 4H 2 O 0.50g / L, Na 2 MoO 4 2H 2 O 0.10g / L, NiCl 2 ·6H 2 O 0.10g / L, ZnCl 2 ·6H 2 O 0.50g / L) and inorganic salt solution 45mL (NaCl0.50g / L, MgCl 2 ·6H 2 O 0.10g / L, CaCl 2 2H 2 O 0.10g / L, NH 4 Cl 0.25g / L, KH 2 PO 4 0.20g / L, KCl0.30g / L, NaHCO 3 2.50g / L, Na 2 S·9H 2 O 0.5g / L)...

Embodiment 2

[0019] The sample comes from the fluid produced from an oil well in Xinjiang Oilfield, and the reservoir temperature is 32°C. 70 μL of mixed alkanes (n-pentadecane, n-hexadecane, n-heptadecane, n-octadecane, n-nonadecane and n-eicosane are uniformly mixed in equal volumes), vitamin solution 0.2mL (vitamin B121.0mg / L, biotin 20.0mg / L, folic acid 20.0mg / L, niacin 50.0mg / L, D-Ca-pantothenate 50.0mg / L, p-aminobenzoic acid 50.0mg / L, vitamin B6-hydrochloric acid 100.0mg / L, riboflavin 50.0mg / L, vitamin B1-hydrochloric acid (2H 2 (2) 50.0mg / L, lipoic acid 50.0mg / L) and trace element solution 0.1mL (CoCl 2 ·6H 2 O 0.50g / L, CuCl 2 2H 2 O 0.10g / L, FeCl 2 4H 2 O 7.50g / L, H 3 BO 31.00g / L, MnCl 2 4H 2 O 0.50g / L, Na 2 MoO 4 2H 2 O 0.10g / L, NiCl 2 ·6H 2 O 0.10g / L, ZnCl 2 ·6H 2 O 0.50g / L) and inorganic salt solution 45mL (NaCl0.50g / L, MgCl 2 ·6H 2 O 0.10g / L, CaCl 2 2H 2 O 0.10g / L, NH 4 Cl 0.25g / L, KH 2 PO 4 0.20g / L, KCl0.30g / L, NaHCO 3 2.50g / L, Na 2 S·9H 2 O 0.5g / ...

Embodiment 3

[0021] The sample comes from a well produced fluid in Shengli Oilfield, and the reservoir temperature is 60°C. 100 μL of mixed alkanes (n-pentadecane, n-hexadecane, n-heptadecane, n-octadecane, n-nonadecane and n-eicosane are uniformly mixed in equal volumes), 0.2 mL of vitamin solution (vitamin B12 1.0 mg / L, biotin 20.0mg / L, folic acid 20.0mg / L, niacin 50.0mg / L, D-Ca-pantothenate 50.0mg / L, p-aminobenzoic acid 50.0mg / L, vitamin B6-hydrochloric acid 100.0mg / L, riboflavin 50.0mg / L, vitamin B1-hydrochloric acid (2H 2 (2) 50.0mg / L, lipoic acid 50.0mg / L) and trace element solution 0.1mL (CoCl 2 ·6H 2 O 0.50g / L, CuCl 2 2H 2 O 0.10g / L, FeCl 2 4H 2 O 7.50g / L, H 3 BO 3 1.00g / L, MnCl 2 4H 2 O 0.50g / L, Na 2 MoO 4 2H 2 O 0.10g / L, NiCl 2 ·6H 2 O 0.10g / L, ZnCl 2 ·6H 2 O 0.50g / L) and inorganic salt solution 45mL (NaCl0.50g / L, MgCl 2 ·6H 2 O 0.10g / L, CaCl 2 2H 2 O 0.10g / L, NH 4 Cl 0.25g / L, KH 2 PO 4 0.20g / L, KCl0.30g / L, NaHCO 3 2.50g / L, Na 2 S·9H 2 O 0.5g / L) to ...

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Abstract

The invention relates to a flora construction method for residual oil gasification exploitation of an oil deposit. The method comprises the following steps of: 1, acquiring an output liquid sample of a producing well of the oil deposit or an active sludge sample polluted by crude oil; 2, adding a hydrocarbon-containing culture medium in the output liquid sample or active sludge sample for carrying out anaerobic enrichment culture; and 3, transferring an enrichment culture obtained in the step 2 into the same hydrocarbon-containing culture medium for carrying out anaerobic culture until the methane is generated. Compared with the prior art, the method can be used for fully utilizing microorganisms in the oil deposit and obtaining the flora which can be used for producing methane through anaerobically degrading hydrocarbon and adapt to the oil deposit environment by directional enrichment, overcomes the deviation and the one-sidedness of the traditional method for manually combining theflora, and lays a foundation for the residual oil gasification development of the oil deposit.

Description

technical field [0001] The invention relates to a method for constructing anaerobic degrading bacterial colony, in particular to a bacterial colony used for gasification exploitation of residual oil in a reservoir and a constructing method thereof. Background technique [0002] It is of strategic significance to increase oil recovery in oil fields in my country. Petroleum is a national strategic resource. In the past 10 years, my country's crude oil consumption has increased at an average annual rate of 5.8%, while the domestic crude oil supply growth rate during the same period was only 1.7%. Since my country became a net importer of crude oil in 1993, the import of crude oil has increased year by year. It is predicted that by 2020, my country's annual oil import demand will exceed 500 million tons, and the degree of dependence on foreign countries will reach 70%. The country will face major economic security problems. Efforts to improve the development level of oilfields...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/28C09K8/582
Inventor 牟伯中刘金峰杨世忠李凯平王立影周蕾
Owner EAST CHINA UNIV OF SCI & TECH
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