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Typing detection kit for influenza virus

A technology of influenza virus and influenza A virus, which is applied in the field of influenza virus typing detection kits, can solve problems such as antibody-specific interference, and achieve the effects of reducing pollution, improving detection efficiency, and high throughput

Active Publication Date: 2012-02-01
DAAN GENE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the experimental process is often interfered by non-specific agglutination factors, antibody specificity, etc., and more stringent requirements are put forward for the standardization of antigens and the preparation technology of monoclonal antibodies.

Method used

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  • Typing detection kit for influenza virus
  • Typing detection kit for influenza virus
  • Typing detection kit for influenza virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Influenza virus typing detection kit and its use

[0028] 1. Prepare a kit including the following components: 1 tube of primer-probe mixture (25 μl / tube), 1 tube of RT-PCR reaction solution (250 μl / tube), 1 tube of RT-PCR reaction enzyme system (75 μl / tube), positive Quality control (200μl / tube) 1 tube, negative quality control (200μl / tube) 1 tube, DEPC H 2 O (2000μl / tube) 1 tube.

[0029] 2. Collection, preservation and transportation of specimens

[0030] 2.1 Specimen types: nasal swabs, throat swabs, nasopharyngeal aspirates and other respiratory samples.

[0031] 2.2 Specimen collection, storage and transportation: Use sterilized cotton swabs to take nasal or throat secretions, and immediately put them into a small test tube containing 2 mL of sterilized normal saline or Eagle solution or 0.5% hydrolyzed milk protein Hanks solution, and label it , put it in a refrigerator and carry it to the laboratory or freeze it at low temperature (-20℃~-70℃). Dry ...

Embodiment 2

[0041] Example 2 Application of Influenza Typing Detection Kit to Detection of Clinical Samples

[0042] Select 3 cases identified as influenza virus negative through the virus culture method, and 3 cases identified as influenza virus positive specimens through the virus culture method, the nucleic acid extraction of the specimen, PCR amplification and result analysis steps are carried out with reference to Example 1, and negative and positive are carried out at the same time Testing of quality control products.

[0043] Test result: the amplification curve of the negative quality control product is not S-shaped (see attached figure 2 ), the amplification curve of the positive quality control product is an obvious S-shaped curve (see attached image 3 , 4, 5), both negative and positive quality control products meet the quality control requirements of the kit, so the test results of the samples to be tested are valid. From the detection results of the specimens to be tested...

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Abstract

The invention relates to a typing detection kit for an influenza virus, especially to a kit for detecting influenza virus A, influenza virus B and influenza virus A (H1N1) with a multiple real-time fluorescent polymerase chain reaction technology. The kit mainly comprises an RT-PCR (real-time polymerase chain reaction) reaction solution, a mixed solution of primer and probe, RT-PCR reaction enzymes, DEPC H2O, and packing boxes for separation and centralized packaging of the reagent bottles or tubes. Able to distinguish influenza virus A, influenza virus B and influenza virus A (H1N1) accurately, the kit of the invention can be widely applied in multiple fields like differential diagnosis and epidemic prevention and control of influenza caused by the above influenza viruses.

Description

technical field [0001] The invention relates to a kit for typing and detecting influenza virus, in particular to a kit for detecting influenza A virus, influenza B virus and influenza A H1N1 virus by using multiple real-time fluorescent polymerase chain reaction technology. The kit can accurately distinguish influenza A, influenza B and influenza A H1N1 viruses, and can be widely used in the differential diagnosis of influenza caused by the above influenza viruses, epidemic prevention and control, and other fields. Background technique [0002] Seasonal influenza is a common acute respiratory infectious disease caused by influenza virus. In the northern part of my country, the incidence of seasonal influenza is high in winter; in the southern part, cases occur in all seasons, and there will be epidemic peaks in summer and winter. Humans are generally susceptible to influenza viruses. Influenza virus is mainly transmitted by coughing or sneezing of an infected person. Some ...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
Inventor 李明陈华云高秀洁
Owner DAAN GENE CO LTD
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