High-sensitivity immunochip detection system and application method thereof

A detection system and high-sensitivity technology, applied in the field of high-sensitivity protein chip detection system, can solve the problems of long detection time, high price, high cost, etc., and achieve the effect of short detection time, low labor intensity and fast detection

Active Publication Date: 2012-02-01
嘉兴艾锐生物科技有限公司
View PDF5 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The radioimmunoassay method can only detect a single index, and it takes 2 to 4 hours to detect a single index, and also requires expensive special equipment such as R-counters, and there are radioactive attenuation and pollution
The detection time of chemiluminescence is short and the sensitivity is high, but it is still an indirect interpretation result of the labeling method. A more objective result interpretation system such as molecular weight has not been introduced, and the detection accuracy is easily affected. The detection of each indicator needs to be specially designed, such as The detection of multiple antibodies has the problems of long detection time and high cost, which is not conducive to the development of grassroots clinical inspection activities

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] A high-sensitivity protein chip detection system, which includes a chip reaction system and a color development system; the chip reaction system includes a carrier, a reaction solution and a washing solution; the color development system includes a detection solution and a stop solution;

[0062] The carrier is coated with probe arrays or quality control points that can specifically bind to biological indicators;

[0063] The reaction solution includes two kinds, one is a biotin marker that can bind to biological indicators, and the other is a complex of horseradish peroxidase and streptavidin;

[0064] The detection solution includes liquid A at pH 4 and liquid B at pH 2, and liquid A includes liquid H 2 o 2 And citric acid-disodium hydrogen phosphate solution, liquid B includes TMB-HCl and sodium citrate-hydrochloric acid solution; Wherein, H 2 o 2 The concentration is 0.005 (v / v)%, the concentration of citric acid-disodium hydrogen phosphate solution is 0.01 moles...

Embodiment 2

[0099] A high-sensitivity protein chip detection system, which includes a chip reaction system and a color development system; the chip reaction system includes a carrier, a reaction solution and a washing solution; the color development system includes a detection solution and a stop solution;

[0100] The carrier is coated with probe arrays or quality control points that can specifically bind to biological indicators;

[0101] The reaction solution includes two kinds, one is a biotin marker that can bind to biological indicators, and the other is a complex of horseradish peroxidase and streptavidin;

[0102] The detection solution includes liquid A at pH 5 and liquid B at pH 3, and liquid A includes liquid H 2 o 2 And citric acid-disodium hydrogen phosphate solution, liquid B includes TMB-HCl and sodium citrate-hydrochloric acid solution; Wherein, H 2 o 2 The concentration is 0.01 (v / v)%, the concentration of citric acid-disodium hydrogen phosphate solution is 0.04 moles ...

Embodiment 3

[0137] A high-sensitivity protein chip detection system, which includes a chip reaction system and a color development system; the chip reaction system includes a carrier, a reaction solution and a washing solution; the color development system includes a detection solution and a stop solution;

[0138] The carrier is coated with probe arrays or quality control points that can specifically bind to biological indicators;

[0139] The reaction solution includes two kinds, one is a biotin marker that can bind to biological indicators, and the other is a complex of horseradish peroxidase and streptavidin;

[0140] The detection solution includes liquid A at pH 5 and liquid B at pH 4, and liquid A includes liquid H 2 o 2 And citric acid-disodium hydrogen phosphate solution, liquid B includes TMB-HCl and sodium citrate-hydrochloric acid solution; Wherein, H 2 o 2 The concentration is 0.05 (v / v)%, the concentration of citric acid-disodium hydrogen phosphate solution is 0.08 moles ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a high-sensitivity protein chip detection system and an application method thereof, belonging to the technical field of biochips. The detection system comprises a chip reaction system and a development system, wherein the chip reaction system comprises a carrier, a reaction liquid and a washing liquid; the development system comprises a detection liquid and a stop liquid; the carrier is coated with probe lattices or quality control points which can be specifically combined with biological indicators; the reaction liquid comprises a biotin label which can be combined with the biological indicators and a compound of horseradish peroxidase and streptavidin; or the reaction liquid is a horseradish-peroxidase-labeled substance which can be combined with the biological indicators; and the stop liquid is a 0.01-10.0(w / v)% sodium azide solution. The invention has the advantages of high detection speed and high sensitivity.

Description

technical field [0001] The invention relates to a high-sensitivity protein chip detection system and an application method thereof, belonging to the technical field of biochips. Background technique [0002] In modern serological detection techniques, in addition to ELISA, radioimmunoassay and chemiluminescence are often used. ELISA method has been widely used in clinical laboratories, especially for the detection of various hepatitis markers. However, due to the complicated operation procedures, the ELISA method takes a long time, and requires expensive laboratory equipment such as microplate readers. The main reason why the ELISA method takes a long time is that the antigen (or antibody) in the liquid phase needs to be diffused to react with the antigen or antibody on the solid phase. Inappropriately shortening the reaction time will reduce the sensitivity below the clinical requirement. Although the one-step rapid detection kit can improve the detection speed, it has th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/531G01N33/532G01N33/535G01N21/76
Inventor 张灿
Owner 嘉兴艾锐生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap