Method for detecting 18 kinds of organic chloride pesticide residues in Chinese medicinal material

A technology for pesticide residues and traditional Chinese medicinal materials, applied in the field of pesticide residue detection, can solve the problems of long residue time, complicated purification, different sample matrices, etc., and achieves the effect of quickness, convenience, cost, and simple process.

Inactive Publication Date: 2012-02-29
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
0 Cites 5 Cited by

AI-Extracted Technical Summary

Problems solved by technology

However, the use of pesticides will also have many negative effects on humans. For example, many organochlorine pesticides are persistent organic pollutants, which have a long residual time in the environment and are potentially harmful to humans. common class of pesticides
[0003] At present, there are many methods for the determination of organochlorine pesticides, but due to various factors such as different samp...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Abstract

The invention discloses a method for 18 kinds of organic chloride pesticide residues in a Chinese medicinal material. In the method, gas chromatography is used for measuring organic chloride pesticides in the sample, wherein the sample is subject to extraction and purification prior to measurement. After organic chloride pesticides in the Chinese medicinal material are extracted by using an organic solvent and concentrated and the solvent is transferred, interfering substances in the extraction liquid are removed by using the concentrated sulfuric acid sulfonation method, and the supernatant liquid is taken by centrifugation and measured by using gas chromatography. The residues in the organic chloride pesticides are calculated through a standard working curve. The method provided by the invention has high measurement accuracy, simple steps, various pesticide kinds and low cost.

Application Domain

Technology Topic

Examples

  • Experimental program(2)

Example Embodiment

[0012] The following embodiments help those skilled in the art to better understand the present invention, but do not limit the present invention in any way. Example 1: Multi-residue analysis of 18 organochlorine pesticides in chrysanthemum
[0013] The chrysanthemum samples were dried in the shade, crushed, and passed through a 60-mesh sieve. Accurately weigh 2.0g sample, add 2.0g anhydrous Na 2 SO 4 , Grind thoroughly, and transfer to a 100mL Erlenmeyer flask with stopper. Add 30 mL of acetone: petroleum ether (1:1) and ultrasonically extract for 15 min, let it stand, and filter the supernatant into a 100 mL pear-shaped bottle. The extraction was repeated twice with 20 mL of acetone: petroleum ether (1:1), and the operation was the same as above. The collected filtrate was concentrated in a 40°C water bath to nearly dryness. Quantitatively transfer the concentrated residue with petroleum ether to a 10 mL graduated test tube with a stopper to make the volume to 5 mL, carefully add 1 mL of concentrated sulfuric acid containing 10% water, shake for 1 min, centrifuge at 3000 rpm for 10 minutes, and take the supernatant Measured by liquid gas chromatograph.
[0014] Results: The average yields of the three levels of this method were 93.2%, 93.4%, 90.7%, and the RSD values ​​were 11.0%, 7.5%, and 6.9%. Conclusion: The method is quick, simple, low cost, and accurate.

Example Embodiment

[0015] Example 2 Multi-residue analysis of 18 organochlorine pesticides in ginkgo leaves
[0016] The ginkgo biloba samples were dried in the shade, crushed, and sieved. Accurately weigh 2.0g sample, add 2.0g anhydrous Na 2 SO 4 , Grind thoroughly, and transfer to a 100mL Erlenmeyer flask with stopper. Add 30 mL of acetone: petroleum ether (1:1) to ultrasonically extract for 15 min, let it stand, and filter the supernatant into a 100 mL pear-shaped bottle. The extraction was repeated twice with 20 mL of acetone: petroleum ether (1:1), and the operation was the same as above. All the collected filtrate was concentrated in a water bath at 40°C to nearly dryness. Quantitatively transfer the concentrated residue with petroleum ether to a 10 mL graduated test tube with a stopper to make the volume to 5 mL, carefully add 1 mL of concentrated sulfuric acid containing 10% water, shake for 1 min, separate the center at 3000 rpm for 10 min, and take the supernatant into Gas chromatography determination.
[0017] Results: The recoveries of the three levels of this method ranged from 71.6% to 130.3%, and the RSD was from 2.2% to 15.8%. Conclusion: The method is simple and quick, and the result is reliable.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

no PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Similar technology patents

Classification and recommendation of technical efficacy words

  • Convenience cost
  • Simple process

Dry-state preservation method for ultra-filtration membrane component

InactiveCN102698605AReduce the difficulty of transportationConvenience costSemi-permeable membranesEnvironmental chemistryFiltration membrane
Owner:SHANDONG ZHAOJIN MOTIAN

Cultivation method considering both machine-harvested cotton plant type shaping and yield in Hebei region

ActiveCN112640756AConvenience costLow costCotton cultivationFertilising methodsNitrogen fertilizerSowing
Owner:COTTON RES INST HEBEI ACAD OF AGRI & FOREST SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products