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Method for detecting 18 kinds of organic chloride pesticide residues in Chinese medicinal material

A technology for pesticide residues and traditional Chinese medicinal materials, applied in the field of pesticide residue detection, can solve the problems of long residue time, complicated purification, different sample matrices, etc., and achieves the effect of quickness, convenience, cost, and simple process.

Inactive Publication Date: 2012-02-29
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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Problems solved by technology

However, the use of pesticides will also have many negative effects on humans. For example, many organochlorine pesticides are persistent organic pollutants, which have a long residual time in the environment and are potentially harmful to humans. common class of pesticides
[0003] At present, there are many methods for the determination of organochlorine pesticides, but due to various factors such as different sample matrices and complicated purification, there are still many problems in daily detection.
Most of the existing technologies are limited to 9 kinds of pesticides such as BHCs, DDTs and PCNB, and the purification treatment is expensive and complicated. The research on the simultaneous analysis of 18 kinds of organochlorine pesticides in Chinese herbal medicines using the sulfonation method has not been reported yet.

Method used

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Examples

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Effect test

example 1

[0012] The following examples help those skilled in the art to better understand the present invention, but do not limit the present invention in any way. Example 1 Multi-residue analysis of 18 organochlorine pesticides in chrysanthemum

[0013] The chrysanthemum samples were dried in the shade, crushed, and passed through a 60-mesh sieve. Accurately weigh 2.0g of the sample, add 2.0g of anhydrous Na 2 SO 4 , fully ground, and transferred to a 100mL Erlenmeyer flask with a stopper. Add acetone:petroleum ether (1:1) 30mL for ultrasonic extraction for 15min, let stand, and filter the supernatant into a 100mL pear-shaped bottle. Then use 20 mL of acetone:petroleum ether (1:1) to repeat the extraction twice, and the operation is the same as above. All the collected filtrates were concentrated in a 40°C water bath to nearly dryness. Quantitatively transfer the concentrated residue to a 10 mL stoppered graduated test tube to 5 mL with petroleum ether, carefully add 1 mL of conc...

example 2

[0015] Example 2 Multi-residue Analysis of 18 Organochlorine Pesticides in Ginkgo Leaf

[0016] Ginkgo biloba samples were dried in the shade, crushed, and sieved. Accurately weigh 2.0g of the sample, add 2.0g of anhydrous Na 2 SO 4 , fully ground, and transferred to a 100mL Erlenmeyer flask with a stopper. Add 30 mL of acetone:petroleum ether (1:1) for ultrasonic extraction for 15 min, let stand, and filter the supernatant into a 100 mL pear-shaped bottle. The extraction was repeated twice with 20 mL of acetone:petroleum ether (1:1), and the operation was the same as above. All the collected filtrates were concentrated in a water bath at 40°C to nearly dryness. Quantitatively transfer the concentrated residue to a 10mL stoppered graduated test tube to 5mL with petroleum ether, carefully add 1mL of concentrated sulfuric acid containing 10% water, shake for 1min, centrifuge at 3000 rpm for 10min, take the supernatant into Gas chromatographic determination.

[0017] Result...

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Abstract

The invention discloses a method for 18 kinds of organic chloride pesticide residues in a Chinese medicinal material. In the method, gas chromatography is used for measuring organic chloride pesticides in the sample, wherein the sample is subject to extraction and purification prior to measurement. After organic chloride pesticides in the Chinese medicinal material are extracted by using an organic solvent and concentrated and the solvent is transferred, interfering substances in the extraction liquid are removed by using the concentrated sulfuric acid sulfonation method, and the supernatant liquid is taken by centrifugation and measured by using gas chromatography. The residues in the organic chloride pesticides are calculated through a standard working curve. The method provided by the invention has high measurement accuracy, simple steps, various pesticide kinds and low cost.

Description

technical field [0001] The invention relates to a method for detecting pesticide residues, which is a sulfonation method for detecting the residues of 18 kinds of organochlorine pesticides in Chinese medicinal materials. Background technique [0002] As we all know, in order to increase the yield of crops, it is necessary to prevent and control diseases and insect pests, and chemical control is the main measure in comprehensive control. For example, during the growth of ginseng, ginseng workers often use DDT, BHC, Pesticides such as pentachloronitrobenzene (PCNB) can eradicate pests and diseases, so the use of pesticides will play an important positive role in increasing agricultural production. However, the use of pesticides will also have many negative effects on humans. For example, many organochlorine pesticides are persistent organic pollutants, which have a long residual time in the environment and are potentially harmful to humans. A common class of pesticides. [0...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 薛健郝丽丽
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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