Surface enhanced Raman substrate suitable for detection of polycyclic aromatic hydrocarbon in seawater

Abstract

The invention relates to a high-sensitivity surface enhanced Roman substrate, which is obtained by adopting the following steps of: functionalizing a quartz plate by utilizing trimethoxy silane, soaking the functionalized quartz plate in gold sol to prepare a gold sol membrane, self-assembling 25, 27-dimercaptoacetate-26,28-dyhydroxyl-4-tertiary butyl calixarene which as special selective adsorption to the polycyclic aromatic hydrocarbon to the surface of the gold sol membrane, and modifying the calixarene. The gold sol substrates with different degree modifications of calixarene can be obtained by controlling the soaking time of the gold sol membrane in a calixarene ethanol solution; and the optimal surface enhanced Raman substrate suitable for field detection of the polycyclic aromatic hydrocarbon is obtained by comparing the enhancement effects of all substrates. Because the calixarene has lyophobic property and can protect a metal nanomaterial from corroding by seawater, therefore the substrate is also suitable for detection of the polycyclic aromatic hydrocarbon in the seawater.

Description

Technical field: [0001] The invention relates to a method for making a high-sensitivity substrate for detecting polycyclic aromatic hydrocarbons in seawater by using surface-enhanced Raman spectroscopy. Background technique: [0002] PAHs are a class of persistent organic pollutants that widely exist in the environment. They mainly come from urban domestic sewage, industrial wastewater discharge, oil pollution, and spontaneous combustion of organic matter. They have the characteristics of high toxicity, persistence, long-distance migration, and high bioaccumulation. , is the earliest known organic compound with carcinogenicity and mutagenicity. Many countries have identified 16 polycyclic aromatic hydrocarbons as environmental hormone pollutants and carcinogenic pollutants that need priority treatment. Therefore, establishing a set of rapid, real-time, simple, practical and easy-to-promote PAH analysis and identification technology has important practical value for China, th...

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Problems solved by technology

[0003] Corresponding to the detection of marine organic pollutants, the main standard chemical analysis instruments recommended by the International Maritime Organization (IMO) include fluorescence spectrometers, gas chromatographs, infrared spectrometers, and color / mass spectrometers. Mass spectrometry uses these methods to analyze instruments that are expensive and cumbersome to operate, and it is impossible to realize real-time in-situ monitoring at sea

Fluorescence spectroscopy has the advantages of high sensitivity, good selectivity, small sample volume, rapid analysis results, and is not affected by time changes. However, for pollutants with complex components, their fluorescence spectra overlap seriously, and data processing is time-consuming and laborious. The accuracy rate is low, and it has not been used as an independent means of identification. Therefore, when this method is actually applied, it needs to be analyzed together with other analysis means.

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