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Oil-emulsion vaccine for poultry and preparation method thereof

An oil emulsion and vaccine technology, applied in the field of vaccines and their preparation, can solve problems such as troublesome adjustment of colloid mill, influence on product quality and titer, system damage, etc., and achieve mass production problems, stability problems, and stable emulsification conditions. Effect

Inactive Publication Date: 2012-03-28
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The stability of oil emulsion vaccines has always been a problem in vaccine production. Due to uneven emulsification, the stability of the emulsion is not good, and it is easy to separate at 2-8°C, which directly affects the quality and potency of the product.
[0014] The commonly used emulsification technology is to use a colloid mill, which mainly controls the radius of the dispersed phase droplet through the rotation speed and tooth spacing. The colloid mill is troublesome to adjust when it is used, the scale is small, it is difficult to realize automatic control, and most of them are open and non-pipeline operations. , not suitable for industrial aseptic production
The second is to use a homogenizer in the tank for emulsification. This homogenizer is suitable for laboratory research and small-scale production. For industrialization that requires a large amount of emulsification, local excessive shearing will occur, resulting in system damage , it is difficult to achieve a uniform emulsification effect

Method used

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  • Oil-emulsion vaccine for poultry and preparation method thereof
  • Oil-emulsion vaccine for poultry and preparation method thereof
  • Oil-emulsion vaccine for poultry and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Prepare 2 million ml Newcastle disease oil emulsion inactivated vaccine.

[0042] Add 632,700 ml of concentrated Newcastle disease antigen to inactivation and add 33,300 ml of Tween-80 for later use. Take 1.2551 million ml (density 0.818g / ml, 1026.7kg) of white oil, first add 65,920 ml of Span-80 (density 0.994g / ml, 65.5kg), add 21.8kg of aluminum stearate, and heat at 120°C Stir well until the solution is transparent. Add it to the first emulsification reaction kettle, when the temperature drops to 35°C, open the emulsification device, and at the same time turn on the cooling water of the emulsification device to make the temperature of the emulsification device 35°C and add the water phase, the flow rate of the water phase is 0.10L / s, the shear rate of the emulsification device It is 2900r / min. After the water phase is added, the emulsification process starts, the flow rate is controlled to 0.5L / s, and the rotation speed of the emulsification device is 2900r / min. In...

Embodiment 2

[0044] Prepare 5 million ml egg drop syndrome oil emulsion inactivated vaccine.

[0045] 1.175 million ml of concentrated EDS antigen was inactivated and 75,000 ml of Tween-80 was added for use. Take 3.5336 million ml (density 0.818g / ml, 2890.5kg) of white oil, first add Span 80 185,600ml (density 0.994g / ml, 184.5kg), add 61.5kg of aluminum stearate, heat at 120°C and stir thoroughly until the solution is transparent. Add it to the first emulsification reaction kettle, when the temperature drops to 35°C, open the emulsification device, and at the same time turn on the cooling water of the emulsification device to make the temperature of the emulsification device 35°C and add the water phase, the flow rate of the water phase is 0.15L / s, the shear rate of the emulsification device It is 2900r / min. After adding the water phase, start the emulsification process, control the flow rate to 0.4L / s, and the rotation speed of the emulsification device to 2900r / min. In the first stage...

Embodiment 3

[0047] Prepare 5 million ml avian influenza oil emulsion inactivated vaccine.

[0048] 1.25 million ml of the concentrated avian influenza antigen was inactivated, and 805,000 ml of Tween was added for later use. Take 3.5336 million ml (density 0.818g / ml, 2890.5kg) of white oil, first add Span 80 185,600ml (density 0.994g / ml, 184.5kg), add 61.5kg of aluminum stearate, heat at 120°C and stir thoroughly until the solution is transparent. Add it to the first emulsification reaction kettle, when the temperature drops to 40°C, open the emulsification device, and at the same time turn on the cooling water of the emulsification device to make the temperature of the emulsification device 40°C and add the water phase, the flow rate of the water phase is 0.08L / s, the shear rate of the emulsification device It is 2900r / min. After adding the water phase, start the emulsification process, control the flow rate to 0.5L / s, and the emulsification device speed to 2900r / min. In the first sta...

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Abstract

The invention discloses an oil-emulsion vaccine for poultry and a preparation method thereof; constant flow emulsification technology is employed; continuous reaction vessel displacement emulsification is performed by using an external emulsification device; through the control of various process conditions, the emulsion is provided with a small liquid bead particle size and less particle size differences, and is distributed uniformly after the emulsion flows through the emulsification device; and thus the emulsion is ensured to be uniform, and the stability of the vaccine is improved. The constant flow emulsification technology is not restricted by batches, has large processing capacity, is applicable to on-line continuous aseptic production, is energy-saving and high-efficient, can run smoothly, and is applicable to tank emulsification procedures for large-scale production. The oil-emulsion vaccine with high stability of the invention has a water-oil volume ratio of 1:1.6-1:4; the water phase comprises vaccine antigens for poultry and 4%-6% of hydrophilic surfactants; the oil phase comprises 94 parts of white oil, 4 parts of lipophilic surfactants, and 2 parts of co-emulsifiers.

Description

technical field [0001] The invention relates to a vaccine and a preparation method thereof, in particular to a poultry oil emulsion vaccine and a preparation method thereof through constant flow emulsification in a tank. Background technique [0002] Oil emulsion inactivated vaccine is a commonly used dosage form in veterinary biological products. It plays an irreplaceable role in the application of vaccines due to its characteristics of continuous immune stimulation and long immune protection period. However, in the production process of oil emulsion inactivated vaccine Due to the influence of factors such as process formula and temperature difference, oil emulsion inactivated vaccines are often unstable, which affects the practical application of inactivated vaccines. [0003] Since the oil emulsion vaccine has a large oil-water interface, it is a thermodynamically unstable system. Its instability is mainly manifested in stratification, rupture, transformation and corrupt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K39/00A61K39/12A61K39/17A61K39/145A61K39/215A61K39/235A61P31/14A61P31/12A61P31/20A61J3/00
Inventor 刘建陈利红韩春贺杨旭贾绍辉
Owner TIANJIN RINGPU BIO TECH
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