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Vaccine for preventing golden pompano ulcer

A technology of golden pomfret and canker disease, which is applied in the field of fish vaccines, can solve problems such as hindering the healthy development of golden pomfret breeding, economic loss, and the impact on the quality of aquatic products, and achieve the effect of preventing golden pomfret canker disease

Active Publication Date: 2012-03-28
TONGWEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, due to the abuse of drugs, the emergence of drug resistance has brought great difficulties to the treatment of the disease, and, due to excessive drug use, the antibiotic residues in the fish body exceed the standard, which affects the quality of aquatic products, resulting in The economic loss has seriously hindered the healthy development of golden pomfret farming in my country

Method used

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  • Vaccine for preventing golden pompano ulcer
  • Vaccine for preventing golden pompano ulcer
  • Vaccine for preventing golden pompano ulcer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The isolation and identification of embodiment 1 bacterial strain

[0020] 1. Experimental materials and methods

[0021] 1.1 Experimental materials

[0022] Isolation and purification of golden pomfret pathogenic bacteria strain: derived from naturally occurring golden pomfret lesions in the deep-sea cage culture base of Lingao County, Hainan, and named JC-1: TW-YYS-17, referred to as JC-1, cultured with TSA Genomic DNA was extracted using a bacterial genomic DNA extraction kit and stored at -20°C for future use. The cultured bacteria were stored in a 4°C refrigerator for later use.

[0023] Reagent consumables: 16S rDNA Bacterial Identification PCR Kit (TAKARA), bacterial genomic DNA extraction kit (Tiangen), sequencing primers (TAKARA), sucrose and other biochemical identification tubes (Hangzhou Microbial Reagent Company), blood agar plate and MacConkey plate (Hangzhou Microbiological Reagent Company), Agarose (TianGen), DNA Maker (TianGen).

[0024] 2. Experime...

Embodiment 2

[0052] Example 2 Comparative study on the pathogenicity of Enterococcus faecalis JC-1 and the standard strain of Enterococcus faecalis (CGMCC 1.2135) to golden pomfret

[0053] 1. Materials and methods

[0054] 1.1 Materials

[0055] 1.1.1 Strains

[0056] Enterococcus faecalis (JC-1) from golden pompano isolated and identified from naturally infected golden pomfret with ulcer disease, the preservation number is CCTCC M 2011071.

[0057] The standard strain of Enterococcus faecalis (CGMCC1.2135) was purchased from China Microorganism Strain Collection Center.

[0058] 1.1.2 Experimental fish

[0059] Experimental fish: healthy golden pomfret (Trachinotusovatus) purchased from Hainan Haifeng deep-sea cage farm, with an average weight of 200.5+5.0g, domesticated in an aquarium for 1 week before the experiment.

[0060] 2. Experimental method

[0061] 2.1 Bacterial culture

[0062] Enterococcus faecalis JC-1 and Enterococcus faecalis standard strain (CGMCC1.2135) were inocu...

Embodiment 3

[0073] Example 3 Comparative study on the immune effect of the inactivated whole-bacteria vaccine prepared by Enterococcus faecalis JC-1 and the standard strain of Enterococcus faecalis (preservation number is CGMCC1.2135)

[0074] 1. Materials

[0075] Strains: Enterococcus faecalis JC-1, isolated and identified from golden pomfrets naturally infected with ulcers, the preservation number is CCTCC M 2011071; the standard strain of Enterococcus faecalis, its preservation number CGMCC1.2135, was purchased from China Microorganism Strains Preservation Management Center.

[0076] Experimental fish: healthy golden pomfret (Trachinotusovatus) purchased from Hainan Haifeng Deep Sea Cage Farm, with an average weight of 400.2±5.9g, domesticated in an aquarium for 1 week before the experiment.

[0077] 2. Experimental method

[0078] (1) Preparation of vaccine

[0079] Enterococcus faecalis JC-1 and Enterococcus faecalis standard strain (CGMCC1.2135) were inoculated on TSA plates res...

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Abstract

The invention discloses a vaccine for preventing golden pompano ulcer, relating to a vaccine. The invention provides an application of Enterococcus faecalis in preparing medicines for preventing golden pompano ulcer, and provides a vaccine. The vaccine is prepared by using Enterococcus faecalis and pharmaceutically acceptable auxiliary materials or carrier. The vaccine provided by the invention can stimulate golden pompano to generate immunity to resist ulcer, and can effectively prevent golden pompano ulcer.

Description

technical field [0001] The invention relates to a fish vaccine, in particular to a vaccine for preventing golden pomfret canker disease. Background technique [0002] The scientific name of golden pomfret is oval pomfret, and the local name is yellow wax pomfret and golden pomfret. Golden Pomfret has the characteristics of delicious meat and rich nutrition, and is very popular among consumers. Therefore, the cultivation of golden pomfret has developed rapidly nationwide, and its cultivation scale and cultivation output have all increased year by year. [0003] But in recent years, a kind of "ulcer disease" characterized by skin ulcers on the body surface, exophthalmos, and bleeding points in the eyes has occurred in the golden pomfret breeding area in Hainan in recent years. It has the characteristics of strong infectivity and high mortality, which has brought huge economic losses to farmers. At the same time, due to the abuse of drugs, the emergence of drug resistance ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/04C12N1/20A61P17/02A61P31/04
Inventor 刘天强肖丹黄冠军阳涛康琦
Owner TONGWEI
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