Quantum dot-polyelectrolyte fluorescent nano complex and application thereof

A polyelectrolyte and fluorescent nanotechnology, which is applied in the field of nanomaterials, can solve the problems of sulfhydryl ligands falling off easily, reducing the fluorescence quantum yield of quantum dots, etc., and achieve the effect of good biocompatibility

Active Publication Date: 2012-03-28
SUZHOU XINGSHUO NANOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a quantum dot-polyelectrolyte fluorescent nanocomposite, which solves the instability problem that its thiol ligands are easy to fall off when the quantum dot is applied, and at the same time does not reduce the fluorescence quantum yield of the quantum dot; The present invention also provides a method for preparing the above-mentioned quantum dot-polyelectrolyte fluorescent nanocomposite

Method used

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  • Quantum dot-polyelectrolyte fluorescent nano complex and application thereof
  • Quantum dot-polyelectrolyte fluorescent nano complex and application thereof
  • Quantum dot-polyelectrolyte fluorescent nano complex and application thereof

Examples

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Embodiment 1

[0049] Take 1ml of the prepared CdTe quantum dot stock solution and place it in an ampoule, add 300μL of 30mg / ml PDADMAC, stir magnetically for 20-30min, add a mixed solution of acetone and absolute ethanol with a volume ratio of 1:1 until the solution is slightly cloudy ( The volume ratio of the reaction solution to the precipitation reagent is about 1:2.5), after shaking evenly, centrifuge at 12,000 rpm for 15 minutes to remove excess PDADMAC, after the centrifugation is complete, discard the supernatant, wash the precipitate twice with three distilled water, The precipitate was ultrasonically redissolved in 1ml triple distilled water to obtain the CdTe-PDADMAC stock solution.

Embodiment 2

[0051] Take 1ml of the prepared CdTe / CdS quantum dot stock solution and place it in an ampoule, add 300μL of 30mg / ml PDADMAC, stir magnetically for 20-30min, add a mixed solution of acetone and absolute ethanol with a volume ratio of 1:1 until the solution is slightly Turbidity (the volume ratio of the reaction solution to the precipitation reagent is about 1:2.5), shake evenly, and centrifuge at 12000rpm for 15 minutes to remove excess PDADMAC. After the centrifugation is complete, discard the supernatant and wash the precipitate twice with three distilled water , ultrasonically redissolve the precipitate in 1ml triple distilled water to obtain the CdTe / CdS-PDADMAC stock solution.

Embodiment 3

[0053] Take 1ml of the prepared CdTe quantum dot stock solution and place it in an ampoule, add 12mg / ml cationic polyelectrolyte PDADMAC monomer DADMAC 200μL, stir magnetically for 20min, then add 10mg / ml polyelectrolyte adjusted to pH=7 with sodium hydroxide. Acrylic acid 200 μL, magnetically stirred for 20 minutes, using a mixed solution of acetone and absolute ethanol with a volume ratio of 1:1 as the precipitation reagent, until the solution was slightly turbid (the volume ratio of the reaction solution to the precipitation reagent was about 1:10), after shaking evenly , 12000rpm high-speed centrifugation for 20min to remove excess DADMAC monomer and PAA. After the centrifugation is complete, discard the supernatant, wash the precipitate twice with triple distilled water, and ultrasonically re-dissolve the precipitate in 1ml triple distilled water to obtain CdTe-DADMAC - PAA stock solution.

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Abstract

The invention belongs to the field of nano materials, and relates to a quantum dot-polyelectrolyte fluorescent nano complex with stable fluorescent property and application thereof. The invention discloses a method for preparing the quantum dot-polyelectrolyte fluorescent nano complex. The quantum dot-polyelectrolyte fluorescent nano complex is formed by self assembly of quantum dots and a polyelectrolyte, has multilayer structure formed by alternately arranging quantum dot layers and polyelectrolyte layers and is in a flexible strip shape, the width of the strip is 2 to 30 nanometers, and the length of the strip is 5 to 400 nanometers. According to the complex, the problem of instability of the quantum dots because sulphydryl ligands of the quantum dots easily drop in application is solved, and the fluorescent quantum yield of the quantum dots is not reduced; and meanwhile, the fluorescent nano complex using the quantum dots is used for preparing SiO2 coated nano particles with high quantum yield, and can be effectively used for biological imaging and biomarkers due to good biocompatibility and high quantum yield.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and relates to a quantum dot-polyelectrolyte fluorescent nanocomposite with stable fluorescent properties and a preparation method and application thereof. Background technique [0002] Before applying quantum dots (QD) to biomarkers, quantum dots must meet the following conditions: first, quantum dots must be able to emit effective fluorescence, and have good stability and low non-specific absorption; second, the surface has active groups Groups, easy to link with biomolecules. The quantum dots synthesized in the water phase mostly use mercapto compounds as ligands. However, due to the weak coordination between the mercapto compounds and the quantum dots, it is easy to fall off from the surface of the quantum dots during common dilution, centrifugation and other experimental processes. Quantum dots generate surface defects, which in turn lead to instability in their fluorescence. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/56C09K11/89C09K11/02
Inventor 谢洪平张丹丹唐建华
Owner SUZHOU XINGSHUO NANOTECH CO LTD
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