Method for evaluating physiological bacterial wilt of paddy rice based on non-invasive micro-detection technology

Abstract

The invention relates to a rapid evaluating method for the physiological bacterial wilt of paddy rice. In the method, ion current of inorganic ions at the roots of the paddy rice seedlings is detected by adopting a non-invasive micro-detection technology; the physiological bacterial wilt can be judged if the ion current flows to paddy rice seedlings which are in the outward flowing state; and theinorganic ions are more than one or two of K<+>, NH<4+> and Ca<2>. The invention further provides application of the non-invasive micro-detection technology to the detection of the physiological bacterial wilt of paddy rice. Due to the adoption of the evaluating and detecting method, non-invasive, active and rapid detection of the physiological bacterial wilt of paddy rice can be realized, the comparison between net ion current of pathogenetic paddy rice seedlings and the net ion current of normal paddy rice seedlings is remarkable, one sample is detected within certain minutes, and the time consumption is low; and compared with a sense identification method, the evaluating method has higher accuracy. The detection method disclosed by the invention is simple and reliable, and has wide application prospect.

Description

technical field [0001] The invention relates to an evaluation method of rice physiological bacterial wilt, in particular to an evaluation method of rice physiological bacterial wilt based on non-damage micro-testing technology. Background technique [0002] Rice bacterial wilt is a common disease of rice. It mostly occurs in the rice seedling stage and the late rice filling stage of rice. The occurrence of physiological bacterial wilt of rice is mostly caused by physiological water loss. In addition, shallow soil, insufficient fertility, and lack of nitrogen fertilizer are also easy to cause the occurrence of physiological bacterial wilt of rice. [0003] When physiological bacterial wilt occurs in rice, the root system is yellow and the root growth is slow. Therefore, as the main organ for absorbing water and nutrients, its absorption function declines, especially the absorption of inorganic ions is the most obvious. Therefore, the detection and evaluation of the root syste...

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Problems solved by technology

[0007] At present, there are few reports on plant disease evaluation. The Chinese patent application number "200910192176" uses banana tissue culture seedlings to inoculate banana fusarium wilt (fusarium wilt), inoculate for 24 days, and root the single plant according to the disease evaluation grade of 0-6. Test-tube seedlings are identified for disease levels; the Chinese patent application number "201010164237" uses a certain amount of inoculum to inoculate the rice to be tested for a certain period of time at a specific growth period of rice under indoor conditions, and cultivates the rice to be tested until the disease is manifested. The severity evaluation of the resistance level of the rice under test to rice black-streaked dwarf disease, and the evaluation of the occurrence of rice physiological bacterial wilt has not been published so far

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