Method for fruiting and breeding needle mushrooms on flat plate through multi-spore self-breeding

Abstract

The invention discloses a method for fruiting and breeding needle mushrooms on a flat plate through multi-spore self-breeding. The method comprises the steps of: collecting spores, mixing monospores for self breeding, fruiting on the flat plate, selecting self-breeded dikaryon colonies and cultivating and screening. The method can be used for greatly increasing the working efficiency and the breeding quality, and the obtained strains have definite target properties and have the characteristics of high growth speed, high yield, attractive appearance, stable heredity, strong stress resistance and the like.

Description

technical field [0001] The invention belongs to the field of edible fungus breeding, and in particular relates to a process of breeding Flammulina velutipes strains on a plate culture medium using a multi-spore self-breeding breeding method. Background technique [0002] Flammulina velutipes is the fastest growing edible fungus species in industrial production in my country. The most frequent breeding application of new varieties of Flammulina velutipes is the breeding method of hybridization and pairing. The disadvantage of this method is that the selection of parents has limitations, that is, the genes carried by the parents showing the target traits are mostly heterozygous genes, or have recessive harmful genes, resulting in unstable inheritance of the target traits, which greatly reduces the Breeding quality and breeding efficiency. [0003] Later, the multispore self-breeding method appeared. The self-bred offspring of Flammulina velutipes will show excellent traits ...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that there is uncertainty in the strain characteristics of the randomly selected bacterial blocks. On the one hand, the proportion of dikaryotic strains is low, which increases the workload of microscopic examination; Without a general understanding, the polyspore self-bred strains cannot be preliminarily screened in advance according to the breeding objectives, and can only be screened after cultivation and fruiting, which reduces the efficiency of selection