Haemophilus parasuis LC strain and application thereof
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A technology of Haemophilus suis and bacteria content, applied in the direction of bacteria, antibacterial drugs, medical preparations containing active ingredients, etc., to achieve the effects of reducing morbidity and mortality, reducing economic losses, preventing epidemics and spreading
Active Publication Date: 2012-04-04
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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[0006] In order to solve the problems of low immune cross-protection and weak immunogenicity between the various serotypes of Haemophilus parasuis commercialized vaccines at home and abroad, The invention provides a highly immunogenic Haemophilus parasuis LC strain
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Embodiment 1
[0027] Haemophilus parasuis ( Haemophilus parasuis ) LC strain, the preservation number of which is CGMCC No. 5257 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms.
[0028] production medium
[0029] Preparation of TSA medium: Weigh 4 g of TSA powder and add it to 100 mL of distilled water, mix thoroughly, sterilize at 121°C for 15 min, cool to 50°C-60°C, add final concentration of 0.001% NAD and 5% newborn bovine serum , mix well and pour into a flat plate for later use.
[0030] Preparation of TSB medium: Weigh 3 g of TSB powder into 100 mL of distilled water, and sterilize at 121 °C for 15 min. It is prepared by adding final concentration of 0.001% NAD and 5% newborn bovine serum before use, and mixing evenly.
[0031] 1.1 Isolation and purification method of HPS strain
[0032] Sick pigs were selected from a large pig farm suspected of Haemophilus parasuis disease in Liaocheng, Shandong Province, and the pericardial effus...
Embodiment 2
[0056] Haemophilus parasuis ( Haemophilus parasuis ) Application of LC strain in the prepared Haemophilus parasuis inactivated vaccine.
[0057] Get the Haemophilus parasuis (Hemophilus parasuis) whose strain preservation number is CGMCC No. 5257 Haemophilus parasuis ) LC strain, inoculate the culture medium after activation, collect the bacterial liquid after culture, inactivate with formaldehyde solution, and mix with aluminum hydroxide gel adjuvant to prepare inactivated aluminum gel vaccine.
[0058] The preferred operation steps are as follows:
[0059] 2.1 Strain selection
[0060] The strain used for making seedlings is HPS LC strain, and its preservation number is CGMCC No.5257. Its colony morphology, bacterial characteristics, biochemical characteristics, and culture characteristics are all stable, and it has strong pathogenicity to pigs. 1.5 mL of bacterial liquid ( 3.0×10 9 CFU / mL) intraperitoneal injection of piglets can cause 80% of piglets to develop disea...
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Abstract
The invention relates to the field of haemophilus parasuis vaccines in veterinary biological products, in particular to a haemophilus parasuis LC strain. The collection number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.5257. The invention also relates to application of the haemophilus parasuis LC strain to preparation of haemophilus parasuis inactivated vaccines. The haemophilus parasuis LC strain has stronger pathogenicity to pigs and has better immunogenicity; an inactivated alumina gel vaccine prepared by the strain is safe and reliable; not only a homologous attacking protection is provided, but also a better cross protection to blood serums type 4, type 5, type 10, type 12, type 14 and type 15 HPS (Hantavirus Pulmonary Syndrome) heterologous attacking can be provided; after the pigs are immunized, a stronger immunity can be generated and the morbidity and the mortality of the inoculated pigs are obviously reduced; the immune effect achieves or is better than the traditional commercialized vaccines in the market; the vaccine has the advantages to compete with like products at home and abroad and is capable of effectively preventing the epidemic and the transmission of a haemophilus parasuis disease and reducing the economic losses caused by the disease, so that the application range is wide.
Description
technical field [0001] The invention relates to the field of Haemophilus parasuis vaccines in veterinary biological products, in particular to a LC strain of Haemophilus parasuis, and also relates to the application of the LC strain of Haemophilus parasuis. Background technique [0002] Haemophilus parasuis ( Haempohlius parasuis , HPS) is a NAD (Nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide, coenzyme Ⅰ)-dependent, non-motile Gram-negative small bacillus, belonging to the Pasteurellaceae (Pasteurellacea) Haemophilus genus (Haemophilus). HPS often causes polyserositis, arthritis and meningitis in pigs, and the disease caused by the bacteria is also known as porcine Grass disease. The bacterium was first isolated by Schermer and Ehrlich in 1922, but it was not until 1992 that the pathogenicity of HPS and the virulence difference between different serotype strains were confirmed by SPF (Specific Pathogen Free) pigs. According to the survey, HPS can ...
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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/102A61P31/04C12R1/21
Inventor 吴家强张玉玉于江杜以军丛晓燕王大鹏王金宝
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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