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Oil reservoir endogenous microorganism high-efficient activator and effect evaluation method thereof

A technology of endogenous microorganisms and activators, applied in the field of microbial biotechnology and resources and environment biology, can solve the problem that the activator has not formed a set of effective methods, and achieve the effect of comprehensive evaluation index system and low operation cost.

Inactive Publication Date: 2012-04-11
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the evaluation of the screened activators and their effects has not formed a set of effective methods

Method used

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  • Oil reservoir endogenous microorganism high-efficient activator and effect evaluation method thereof
  • Oil reservoir endogenous microorganism high-efficient activator and effect evaluation method thereof
  • Oil reservoir endogenous microorganism high-efficient activator and effect evaluation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 activator solid water insoluble content determination

[0052] Refer to the standard SY / T 5329-94 clastic reservoir injection water quality recommended indicators and analysis methods. Soak the filter membrane in distilled water for 30 minutes, and wash it with distilled water for 3-4 times; take out the filter membrane and place it in an oven for 30 minutes at 90°C, take it out, put it in a desiccator to cool to room temperature, and weigh it; the activator (corn steep liquor Dry powder 0.33%, diammonium hydrogen phosphate 0.3 and potassium nitrate 0.2%) aqueous solution is packed in the microporous membrane filtration tester; After the filter membrane of constant weight is wetted with water, be packed on the microporous filter; Vacuumize, make pumping Keep the pressure in the filter device at 0.1-0.15MPa, filter, and record the outflow volume; take out the filter membrane from the filter with tweezers, dry and weigh it; bring it into the formula to calcul...

Embodiment 2

[0053] The economic and technical feasibility of embodiment 2 activator application

[0054] The activator components of Shengli Oilfield Zhan 3 Blocks are all solid powder particles, which are convenient for transportation and storage, and solve the problems of poor fluidity of liquid raw materials and difficulty in batching in winter; the content of solid water insolubles is 4 mg / l, which meets the water quality requirements of oilfield water injection; The selected components are all compound nutrients, and have a certain pH buffering effect, which provides a good nutritional and reproductive environment for the growth of microorganisms; there are few types of activator components, which reduces the purchase and transportation links; currently, corn steep liquor dry powder The market price is 2500 yuan·t -1 , diammonium hydrogen phosphate 4000 yuan · t -1 , sodium nitrate 2800 yuan · t -1 , with 3 pieces of activator (0.33% corn steep liquor dry powder, 0.3% diammonium hy...

Embodiment 3

[0055] The detection of embodiment 3 microbial selective activation index

[0056] Referring to the SY / T0532-93 oilfield injection water bacterial analysis method, the extinction dilution method was used to determine the total bacterial concentration, the number of hydrocarbon oxidizing bacteria, sulfate reducing bacteria and methanogenic bacteria using a bacterial test bottle.

[0057] After the activator activated the 3 pieces of oil and water samples in Shengli Oilfield, the microbial community structure was significantly strengthened and improved (see Table 3): the total bacterial concentration reached 10 8 cells·ml -1 , the number of hydrocarbon-degrading bacteria increased to 10 7 cells·ml -1 , the number of methanogens increased to 10 4 cells·ml -1 , the number of sulfate-reducing bacteria was effectively controlled.

[0058] Table 3 Changes in the number of microorganisms in oil and water samples before and after activation

[0059]

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Abstract

The invention relates to an oil reservoir endogenous microorganism high-efficient activator and an effect evaluation method thereof. The method comprises: the evaluation of activator components based on the economic and technical feasibility of the application of the activator; the evaluation of the activation effect of the activator based on the selective activation of oil reservoir endogenous microorganisms, the changes of biochemical indexes of oil and water samples, and the extent of the improvement of the crude oil recovery efficiency by physical simulation oil displacement experiments; the construction of detailed scoring rules of the activator and its effect with respect to the economic and technical feasibility, the selective activation of endogenous microorganisms, the biochemical indexes of oil and water samples, and physical simulation oil displacement; the construction of grade division methods of the activator and its effect based on the scoring conditions of each index. The evaluation method of the invention has the advantages of systemic and comprehensive evaluation indexes, low operation cost, and subsequent referential evaluation indexes, solves the current problems of the lack of an oil reservoir endogenous microorganism high-efficient activator and its standardized evaluation method, and provides an systemic and effective evaluation method for the smooth oil field application of the oil reservoir endogenous microorganism high-efficient activator.

Description

technical field [0001] The invention relates to a high-efficiency activator for oil reservoir endogenous microorganisms and an evaluation method for its effect, belonging to the fields of microbial biotechnology and resource environment biotechnology. Background technique [0002] As many major oil fields in the world have entered the high water cut period, they are facing problems such as difficult mining and high mining costs. Microbial oil recovery technology has the advantages of low cost, strong adaptability, low damage to production layers, and no environmental pollution. The huge potential of enhanced oil recovery in oil reservoirs after chemical flooding and depleted oil reservoirs has drawn widespread attention from all over the world. Compared with the exogenous microbial oil recovery technology, the endogenous microbial oil recovery technology does not need to inject microorganisms into the formation, but only needs to inject nutrients into the reservoir and injec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K8/582E21B43/22
Inventor 马挺高配科李国强赵玲侠曹美娜高梦黎
Owner NANKAI UNIV
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