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Agent for regulating composition ratio of intestinal bacterial flora

A technology of intestinal bacteria and regulators, applied in the direction of plant/algae/fungus/moss ingredients, food ingredients, organic active ingredients, etc., can solve the problems of burden, no proliferation of Bacteroides bacteria, reduction, etc., and achieve no side effects , improve the immune index, reduce the effect of allergic symptoms

Inactive Publication Date: 2012-04-11
FUJIFILM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, in order to achieve the effect of ingesting fructo-oligosaccharides, a large amount of intake is required, and ingesting it through diet or functional foods places a burden on the body
In addition, oral substances that have the effect of proliferating Bacteroides bacteria and also reducing Firmicutes genus bacteria have not been found

Method used

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  • Agent for regulating composition ratio of intestinal bacterial flora
  • Agent for regulating composition ratio of intestinal bacterial flora

Examples

Experimental program
Comparison scheme
Effect test

test approach 1

[0035] Sucrase IC 50 Determination of value

[0036] Preparation of sample solution: 2 mg of a sample (substance that inhibits α-glucosidase activity) was weighed into a test tube, and 2 mL of water was added thereto, and then the sample was completely suspended to form a sample solution having a concentration of 1 mg / mL. It was diluted with water to form solutions at 0, 50, 100, 250 and 500 μg / mL, respectively.

[0037] Preparation of substrate solution: Sucrose was dissolved in 0.2M maleate buffer (pH 6.0) so that the sucrose concentration was 100 mM, and the solution was used as a substrate solution.

[0038] Preparation of crude enzyme solution: After suspending 1 g of rat intestinal acetone powder rat (manufactured by SIGMA) in 10 mL of physiological saline, the suspension was centrifuged (3000 rpm, 4° C., 5 min). The resulting supernatant was separated and used as crude enzyme solution.

[0039] To 500 μL of the above sample solutions having respective concentrations ...

Embodiment

[0099] The present invention will be described below with reference to examples, but the present invention is not limited to the following examples.

[0100] After pulverizing the root and stem parts of Salacia reticulata and Salacia oblongatus, they were mixed in equal weight, and the liquid obtained through the hot water extraction step at 98°C was spray-dried to obtain Salacia extract powder1. Salacia extract powder 1 has a sucrase IC50 value of 41 (μg / ml).

[0101] In addition, for Gymnema and mulberry leaf extracts, those available from K.K. Cross can be used.

[0102] Also, for the comparative example, glucose, lard, and oolong tea polymerized polyphenols (obtained by freeze-drying black oolong tea produced by Suntory Ltd. and extracting oolong tea polymerized polyphenols) were used. In addition, for Gymnema, mulberry leaf extract, glucose, lard, and oolong tea polyphenols, their sucrase 50% inhibitory concentrations (IC 50 value) of 800 μg / mL or greater.

[0103] Th...

experiment example 1

[0105] Eight-week-old male SD rats were divided into seven rat groups. Each of the following intestinal bacterial flora composition ratio regulators was dissolved in water for injection, and ingested over a week in an amount equivalent to 20 mg / kg / day using a gastric feeding tube (stomach sonde). The ratio of bacteria of the genus Bacteroidetes and Firmicutes to the intestinal bacterial community was determined by the T-RFLP method (Nagashima method) immediately before and immediately after ingestion, respectively. However, in Example 1 half the amount (10 mg / kg / day) of intestinal bacterial flora constituted the ratio regulator was used.

[0106] Table 1 shows the proportions of bacteria belonging to the genus Bacteroides and Firmicutes relative to the intestinal bacterial flora (the proportion obtained when the total number of bacteria is taken as 100%).

[0107]

[0108] As for the results of this experimental example, it shows that the substance that inhibits sugar abso...

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Abstract

Disclosed is a food or a drug whereby the composition ratio of intestinal bacterial flora can be safely regulated with little side effects. An agent for regulating the composition ratio of intestinal bacterial flora characterized by containing at least one kind of sugar absorption inhibitor, and a food or a drug containing the same.

Description

technical field [0001] The present invention relates to an intestinal bacterial flora composition ratio regulator comprising a substance that inhibits sugar absorption. Background technique [0002] According to recent studies, it has been found that in humans, obese people are in a state in which the proportion of Bacteroidetes bacteria is low and the proportion of Firmicutes bacteria is high. increased, but on the contrary, the proportion of Firmicutes decreased. Furthermore, it has been found that lean persons have a high composition ratio of Bacteroidetes and a low composition ratio of Firmicutes group relative to obese persons (see Non-Patent Document 1). [0003] Furthermore, when the gut bacterial flora of obese mice and that of normal-weight mice were transplanted into two groups of mice without gut bacteria, it was found that the gut bacteria of obese mice transplanted The body weight of the group of mice was significantly larger than that of the group of mice tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A23L1/30A61K36/18A61K36/60A61P1/14A61P11/02A61P35/00A61P37/04A61P37/08A61P43/00
CPCA61K31/00A61K36/605A61K36/37A61K36/27A23L1/3002A61K2236/331A23V2002/00A61K2300/00A23V2200/332A23V2250/21A23L33/105A61P1/00A61P1/14A61P11/02A61P3/04A61P35/00A61P37/04A61P37/08A61P43/00A23V2200/30
Inventor 小田由里子植田文教
Owner FUJIFILM CORP
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