Determination reagent against bilirubin interference based on uricase method and application method thereof

A technology of uricase and bilirubin, which is applied in the biological field to achieve excellent stability, high sensitivity, and good anti-bilirubin interference performance

Inactive Publication Date: 2012-04-18
ZHEJIANG CENTURY CONDOR MEDICAL SCI & TECHNOLOTY CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Determination reagent against bilirubin interference based on uricase method and application method thereof
  • Determination reagent against bilirubin interference based on uricase method and application method thereof
  • Determination reagent against bilirubin interference based on uricase method and application method thereof

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Embodiment 1

[0041] (1) Sample preparation: the sample is fasting non-hemolyzed serum or plasma (heparin anticoagulation, 0.1mg heparin can anticoagulate 1.0ml blood; EDTA anticoagulation: 1.8mg EDTA can anticoagulate 1.0ml blood). Samples should be transported and stored at low temperature. Uric acid in samples is stable for 8 hours at room temperature, 3 days at 2°C to 8°C, and 6 months at -20°C.

[0042] (2) Prepare the uric acid determination reagent according to the following ingredients and dosage:

[0043] Reagent 1:

[0044] Phosphate buffer (pH 6.0-8.0) 0.10-100g / L,

[0045] Peroxidase 0.10-10KU / L,

[0046] N-ethyl-N-(3-toluene)-N-succinylethylenediamide EMSE 0.10-10g / L,

[0047] Anti-interference enzyme 0.10-10KU / L,

[0048] Stabilizer 0.10-100g / L;

[0049] Reagent 2:

[0050] Phosphate buffer (pH 6.0-8.0) 0.10-100g / L,

[0051] Peroxidase 0.10-10KU / L,

[0052] Uricase 0.10-10KU / L,

[0053] 4-Aminoantipyrine 4-AAP 0.10-10g / L,

[0054] Stabilizer 0.10-100g / L.

[0055]The...

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Abstract

The invention relates to a determination reagent against bilirubin interference based on a uricase method and an application method thereof. The reagent is a liquid double-reagent and has good resistance against bilirubin interference and excellent stability. The reagent can be used in a semi/full-automatic biochemical analyzer by adopting a two-point end assay method. By adopting a specific enzyme, the reagent has the characteristics of high sensitivity and strong anti-interference capability; and by adding a selected stabilizing agent, the reagent achieves good stability, namely, the reagent can be hermetically preserved at the temperature of 2-8 DGE C in dark place for 12 months without causing performance deterioration. The reagent can be widely applied to diagnosis, of kidney diseases such as acute and chronic nephritis, kidney stone, gout and the like as well as diseases resulting from excessive nucleic acid metabolism, such as leukemia, multiple myeloma and the like, physical examination and general investigation of kidney diseases and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a uricase assay reagent for anti-bilirubin interference and a use method thereof. Background technique [0002] Uric acid (UA) is the final product of purine metabolism in nucleic acid. In the human body, purine nucleotides are decomposed to generate purine nucleosides and purines, which are deaminated and oxidized by hydrolysis to finally generate UA. UA is excreted with urine, and all UA in the blood is filtered out by the glomeruli and almost completely reabsorbed in the renal tubules, so the clearance rate of UA is extremely low. The UA excreted by the kidneys accounts for 2 / 3-3 / 4 of the total daily excretion, and the rest is decomposed by microbial enzymes in the gastrointestinal tract. When GFR is reduced, UA cannot be excreted normally, and the concentration of UA in blood increases. Some drugs also affect the excretion of UA, such as thiazide diuretics and hydroxysulfanamide ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/28C12Q1/26G01N33/49
Inventor 王浩余向东程明汪峰
Owner ZHEJIANG CENTURY CONDOR MEDICAL SCI & TECHNOLOTY CORP
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