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Method for separating and concentrating L-theanine from glutaminase or glutamyl transpeptidase conversion liquid by membrane integration technology

A technology of glutamyl transpeptidase and glutaminase, applied in the field of separation and extraction of L-theanine

Active Publication Date: 2012-05-02
JIANGSU AGRO BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from the analysis of operation process, separation and concentration, product purity, and economic benefits, the balanced activation of ion exchange resin, impurity elution, and regeneration cleaning all need to consume a large amount of acid and alkali and water, and the required equipment and resin costs are high. At the same time, a large amount of difficult-to-treat wastewater is produced. In addition, the yield of L-theanine is not high (generally less than 70%) and the product purity is low. Therefore, a high-yield, low-cost L-theanine separation and concentration method is developed. It is very urgent to promote the industrial production of L-theanine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (a) Take 100 liters of L-theaninase conversion solution, the concentration of L-theanine is 15.4 g / L, and 0.5 m 2 The tubular ceramic ultrafiltration membrane is filtered at 0.2Mpa, the temperature is 32°C, and the pH is 10.5. When the membrane pore size is 0.001-0.01um and the volume of the retained liquid is 15L, add 10L of purified water to dilute and then filter, repeat 3 times Afterwards, obtain 113 liters of ultrafiltration membrane permeate liquid, and L-theanine concentration is 13.5 grams per liter in the ultrafiltration membrane permeate liquid;

[0029] (b) Filter 113 liters of ultrafiltration membrane permeate with a polyamide nanofiltration membrane with a pore size molecular weight cut-off of 300 under the conditions of a pressure of 1.5 MPa, a temperature of 30 ° C, and a pH of 9.0. The volume of the retained liquid is about 20 L , add 20L deionized water to dilute and filter again, after repeating 3 times, obtain nanofiltration membrane permeate liquid 1...

Embodiment 2

[0033] (a) Take 100 liters of L-theaninase conversion solution, the concentration of L-theanine is 15.4 g / L, and 0.5 m 2 The tubular polyvinylidene fluoride ultrafiltration membrane is filtered at 0.3Mpa, the temperature is 25°C, and the pH is 10.5. The membrane pore size is 0.001-0.01um, and when the volume of the retained liquid is 15L, dilute with 10L of distilled water and then filter. After repeating 3 times, 118 liters of the ultrafiltration membrane permeate was obtained, and the L-theanine concentration in the ultrafiltration membrane permeate was 13.0 grams per liter;

[0034] (b) Filter 118 liters of ultrafiltration membrane permeate with a polyamide nanofiltration membrane with a pore size molecular weight cut-off of 400 under the conditions of a pressure of 2.0 MPa, a temperature of 25 °C, and a pH of 9.5. The volume of the retained liquid is 20 L , add 20L of purified water to dilute and then filter, after repeating 3 times, 150 L of nanofiltration membrane permea...

Embodiment 3

[0038] (a) Take 100 liters of L-theaninase conversion solution, the concentration of L-theanine is 15.4 g / L, and 0.5 m 2The tubular polysulfone ultrafiltration membrane is filtered at 0.35MPa, the temperature is 30°C, and the pH is 11.5. When the membrane pore size is 0.001-0.01um and the volume of the retentate is 15L, add 10L of purified water to dilute and then filter, repeat 3 times After the second time, 122 liters of ultrafiltration membrane permeated liquid were obtained, and the L-theanine concentration in the ultrafiltration membrane permeated liquid was 12.5 grams per liter;

[0039] (b) Filter 122 liters of ultrafiltration membrane permeate with a polyamide nanofiltration membrane with a pore size molecular weight cut-off of 500 at a pressure of 1.8 MPa, a temperature of 35°C, and a pH of 9.0. The volume of the retained liquid is 15 L , add 15L of purified water to dilute and filter, repeat 3 times to obtain 155 L of nanofiltration membrane permeate, and the concent...

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PUM

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Abstract

The invention relates to a method for separating and concentrating L-theanine from glutaminase or glutamyl transpeptidase conversion liquid by a membrane integration technology. The method comprises the following steps of: (a) filtering the enzymatic conversion liquid by using an ultrafiltration membrane with the pore size of 0.001 to 0.01mu m, retaining and removing thalli, cell debris and macromolecular heteropolymeric protein in the enzymatic conversion liquid, and filtering to obtain ultrafiltration membrane permeate; (b) filtering the ultrafiltration membrane permeate by using a polyamide nanofiltration membrane, desalting the ultrafiltration membrane permeate, decolorizing, retaining micromolecular heteropolymeric protein and substances with the molecular weight of 200 to 1,000, and filtering to obtain nanofiltration membrane permeate; (c) concentrating and extracting the nanofiltration membrane permeate by using a reverse osmosis membrane to obtain a concentrated solution; and d) concentrating the concentrated solution in vacuum, and crystallizing to obtain an L-theanine crystal. The extraction process is a membrane technology separation process, the method is easy to operate, automation is easy to realize, yield and product purity are high, energy consumption is low, and a few pollutants are generated.

Description

technical field [0001] The invention relates to a method for separating and extracting L-theanine, in particular to a method for separating and concentrating L-theanine from an enzyme conversion liquid obtained by converting L-theanine by microbial fermentation enzymes by using integrated membrane technology. Background technique [0002] L-theanine (N-ethyl-γ-glutamine, theanine) is a natural amino acid, a characteristic amino acid in tea, and an effective taste substance in tea. L-theanine is mainly used for: (1) As an emerging food additive, it can be used as a quality improver for tea drinks, an additive for improving food flavor, an additive for functional food, and an additive for "emotional food". It has been widely used in Europe, America, Japan, China Taiwan and other countries and regions. For example, Japan has developed chocolate, jelly, pudding, chewing gum, health tea and various refreshing drinks with theanine added. (2) In the field of pharmaceutical inter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C237/06C07C231/24
Inventor 王汉领沈青红徐大春吕志祥万红贵殷志敏
Owner JIANGSU AGRO BIO TECH