Method for transforming hyperin into quercetin by enzyme reaction

A technology of hyperin and quercetin, which is applied in directions such as fermentation, can solve problems such as low quercetin content and low yield, and achieves expansion of processability and practical application range, improvement of conversion rate, and improvement of pharmacological activity. Effect

Inactive Publication Date: 2012-05-16
许明淑 +2
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  • Claims
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Problems solved by technology

However, the content of quercetin in natural plan...

Method used

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  • Method for transforming hyperin into quercetin by enzyme reaction
  • Method for transforming hyperin into quercetin by enzyme reaction
  • Method for transforming hyperin into quercetin by enzyme reaction

Examples

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example 1

[0023] Example 1: In 1000mL Erlenmeyer flask, add 10mg hyperin standard substance and 400ml30% ethanol aqueous solution, adjust pH value to 6.0 with 1M NaOH solution, system reaches certain temperature 50 ℃, then add 0.5g naringinase (standard activity 475AGUPg), and finally, at 50° C. and 200 rpm, the reaction was stirred for 30 hours. Under the same conditions, no enzyme was added as the control group. After the reaction is finished, extract with the same volume of ethyl acetate to remove carbohydrates and enzyme protein impurities, and the extract is developed on a G silica gel plate (100mm × 25mm), with ethyl acetate: formic acid=25: 1 as the mobile phase. Observe under 365nm ultraviolet lamp and carry out qualitative analysis with TLC method, the result is as follows figure 1 Shown (1.R f金丝桃苷标准品 =0.36 2.R f反应产物 =0.90 3.R f对照组 =0.36), the reaction product R f The value 0.90 is much larger than the standard R f A value of 0.36 shows that quercetin with a polarity les...

example 2

[0026] Example 2: In the 1000ml Erlenmeyer flask, add 10mg hyperin standard substance and 400ml40% ethanol aqueous solution, adjust pH value to 5.0 with 1M NaOH solution, system reaches certain temperature 40 ℃, then add 0.5g naringinase (standard activity 475AGUPg), and finally, at 40° C. and 200 rpm, the reaction was stirred for 30 hours. Under the same conditions, no enzyme was added as the control group. After the reaction, extract with the same volume of ethyl acetate to remove carbohydrates and enzyme protein impurities. The extract is a G silica gel plate (100mm×25mm), ethyl acetate: formic acid: water=30:2:3 as mobile phase Expand, observe under 365nm ultraviolet light and carry out qualitative analysis with TLC method, the result is as shown in Figure 2 (1.R f金丝桃苷标准品 =0.44 2. R f反应产物 =0.923.R f对照组 = 0.44). Reaction product R f The value 0.92 is much larger than the standard R f A value of 0.44 indicates that quercetin, which is less polar than hyperin, has been ...

example 3

[0027] Example 3: In the 1000ml conical flask, add 10mg hyperin standard substance and 400ml 50% ethanol aqueous solution, adjust pH value to 6.0 with 1M NaOH solution, system reaches certain temperature 60 ℃, adds 0.5g naringinase (standard Activity 475AGUPg), finally, at 60°C and 200rpm, the reaction was stirred for 30 hours. Under the same conditions, no enzyme was added as the control group. After the reaction finishes, remove carbohydrates and enzyme protein impurities with ethyl acetate extraction of the same volume, the extract is developed with ethyl acetate: formic acid=25: 1 as the mobile phase, observe under a 365nm ultraviolet lamp and carry out qualitative analysis with TLC analysis, the result is as figure 1 Shown (1.R f金丝桃苷标准品 =0.36 2. R f反应产物 =0.90 3.R f对照组 =0.36), the reaction product R f The value 0.90 is much larger than the standard R f A value of 0.36 indicates that quercetin, which is less polar than hyperin, has been produced by the enzymatic reac...

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Abstract

The invention relates to a method for transforming hyperin into quercetin by using a biological enzyme reaction to improve the biological activity of the hyperin. According to the method, a reaction is performed in an ethanol aqueous solution with the concentration of 30-70% under an effect of naringinase, the reaction temperature is 40-70 DEG C, the reaction time is 1-30 hours, and the glycosyl on the hyperin hydroxyl is cut, such that the hyperin is transformed into the quercetin with the high pharmacological activity. With the present invention, the workability of the hyperin is further improved, and the practical application range of the hyperin is expanded.

Description

technical field [0001] The invention relates to a method for converting hyperoside into quercetin, in particular to a method for obtaining quercetin by enzyme reaction. Background technique [0002] The molecular formula of Hyperin is C 21 h 20 o 12 , the chemical structural formula is as shown in formula I. Pale yellow needle crystal. [0003] [0004] (Formula I) [0005] Hyperin is derived from natural products. The natural active substance hyperin and its aglycon quercetin have great development value. Among them, hyperoside is mostly used for analgesia and antispasmodic, while quercetin is mostly used for the treatment of clinical cardiovascular diseases. [0006] Hyperoside has a strong analgesic effect and exhibits antispasmodic effects both in vivo and in vitro; hyperoside also has a significant protective effect on cerebral ischemia-reperfusion injury. Hyperin also has a sedative effect. [0007] However, in terms of pharmacological activity, the medical...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 许明淑赵无恙张丽娟
Owner 许明淑
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