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Method for extracting oleuropein from olive leaves

A technology of oleuropein and olive leaf, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of high cost and large amount of organic solvents, and achieve low cost, reduced dosage, and reduced energy consumption. consumption effect

Inactive Publication Date: 2012-05-23
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process uses a large amount of organic solvents and is costly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Crush olive leaves, take 1kg, add 8kg of saturated lime water and heat to 50°C, soak for 3 hours to extract, filter out the liquid, add 8kg of saturated lime water for extraction for 2 hours, combine the two extracts, adjust the pH to neutral with hydrochloric acid, filter , add 1LAB-8 macroporous resin column for adsorption, after the adsorption is over, use 5L of water → 5L of 30% ethanol → 6L of 60% ethanol solution to elute in sequence, collect the 60% ethanol eluate and concentrate it under reduced pressure to a density of 1.07, transfer it to a beaker and add 100ml Stir the water-saturated n-butanol evenly, pour it into a separatory funnel to separate layers, collect the n-butanol, and extract 4 times, combine the n-butanol layers to recover the n-butanol under reduced pressure, and obtain an extract. Add ethanol to the extract and dissolve it under reflux until the alcohol concentration is 70%, then add 5 g of activated carbon for medicinal injections to decoloriz...

Embodiment 2

[0026] Crush olive leaves, take 1kg, add 10kg of saturated lime water and heat to 40°C, soak for 2 hours to extract, filter out the liquid, add 8kg of saturated lime water for extraction for 2 hours, combine the two extracts, adjust the pH to neutral with sulfuric acid, filter , add 1L LSA-8 macroporous resin column for adsorption, after the adsorption is over, use 8L water → 5L 30% ethanol → 6L 50% ethanol solution to elute in sequence, collect the 50% ethanol eluate and concentrate it under reduced pressure to a density of 1.05, transfer it to a beaker and add 200ml of water-saturated n-butanol was stirred evenly, poured into a separatory funnel to separate layers, collected n-butanol, extracted 3 times, combined the n-butanol layer and recovered n-butanol under reduced pressure to obtain an extract. Add ethanol to the extract and dissolve it under reflux until the alcohol concentration is 80%, then add 7g of activated carbon for medicinal injections to decolorize for 1 hour,...

Embodiment 3

[0028] Crush olive leaves, take 1kg, add 8kg of saturated lime water, heat to 60°C, soak and extract for 3 hours, filter out the liquid, add 8kg of saturated lime water for extraction for 2 hours, combine the two extracts, adjust the pH to neutral with hydrochloric acid, filter , add 1L SPD-700 macroporous resin column for adsorption, after the adsorption is completed, use 6L water → 6L 20% ethanol → 5L 70% ethanol solution to elute in sequence, collect the 70% ethanol eluate and concentrate it under reduced pressure to a density of 1.04, transfer it to a beaker and add 200ml of water-saturated n-butanol was stirred evenly, poured into a separatory funnel to separate layers, collected n-butanol, extracted 3 times, combined the n-butanol layer and recovered n-butanol under reduced pressure to obtain an extract. Add ethanol to the extract and dissolve it under reflux until the alcohol concentration is 90%, then add 10 g of active carbon for medicinal injections to decolorize for ...

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Abstract

The invention relates to a method for extracting oleuropein from olive leaves. The method comprises the following steps of: smashing olive leaves; adding saturated lime water in an amount of 8-15 times that of the olive leaves, and heating and extracting for 2-3 times; adjusting an extracting solution to be neutral; adding a filtered extracting solution into a macroporous resin column for adsorbing; gradually eluting with ethanol water; collecting active ingredients; concentrating under reduced pressure; adding water-saturated n-butyl alcohol into a concentrated solution for extracting for 3-5 times; collecting a n-butyl alcohol layer; recovering an organic solvent under reduced pressure to obtain an extract; adding ethanol for refluxing and dissolving; adding active carbon for decoloring for 1-2 hours; filtering the active carbon out; recovering ethanol; and performing freeze drying to obtain the oleuropein product. The method has the advantages of easy operating, no use of toxic reagents in a preparation process, environmental friendliness and high product content, and is suitable for industrial production.

Description

Technical field: [0001] The invention belongs to the field of separation of natural products, in particular to a method for extracting oleuropein from olive leaves. Background technique: [0002] Oleuropein is a phenolic split iridoid glycoside, molecular formula: C 25 h 32 o 13 , molecular weight 540.43, soluble in hot water, methanol, ethanol, ethyl acetate, etc., insoluble in chloroform, petroleum ether and other non-polar organic reagents. [0003] Oleuropein is a polyphenol compound with antioxidant capacity, which can reduce the oxidation degree of low-density lipoprotein, prevent coronary heart disease and atherosclerosis, and can also relieve vascular smooth muscle and lower blood pressure. [0004] Olive leaves are the mature leaves of Oleaceae olives, the fruit seeds are rich in nutrition, and the leaves are mostly waste, but the leaves contain a lot of antioxidant components, and oleuropein is one of the active components. With the development of modern medica...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 李法庆杨成东刘东锋
Owner 苏州宝泽堂医药科技有限公司
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