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Fabrication procedure of pathogenic microorganism infection diagnosis type cell chip

A technology of pathogenic microorganisms and cell chips, which is applied in the field of production procedures of pathogenic microorganism infection diagnostic cell chips, can solve the problems of slow detection speed, poor accuracy, high cost, etc., and achieve the effects of easy mastery, low cost and low cost

Inactive Publication Date: 2012-05-30
DALIAN CHUANGDA TECH TRADE MARKET
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention solves the technical problems of low throughput, high cost, slow detection speed and poor accuracy in the background technology

Method used

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  • Fabrication procedure of pathogenic microorganism infection diagnosis type cell chip
  • Fabrication procedure of pathogenic microorganism infection diagnosis type cell chip
  • Fabrication procedure of pathogenic microorganism infection diagnosis type cell chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] see figure 1 , 2 , 3, the pathogenic microorganism infection diagnosis type cell chip of the present invention is mainly made of substrate 1,

[0039] The reaction tank 2 and the cell sheet 3 are composed. The substrate 1 can be made of organic polymer material, metal or silicon, etc., and the reaction tank 2 for placing the cell sheet 3 is processed on it, and the reaction tank 2 can be one, two or more according to the detection requirements, so as to be arranged as an array. More than one cloth is better. The cell sheet 3 is a cell sheet 3 corresponding to the shape of the reaction tank 2 cut from a bacterial smear of pathogenic microorganisms such as bacteria, fungi, and spirochetes or a cell sheet coated with virus-infected eukaryotic cells, the material of the cell sheet It can be glass, plastic or nitrocellulose film, etc. The cell sheet 3 is embedded in the reaction tank 2 . The cell sheet is coated with a specific antigen for a certain pathogenic microorga...

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Abstract

The invention discloses a fabrication procedure of a pathogenic microorganism infection diagnosis type cell chip, which comprises the following steps of: arranging a reaction tank on a substrate of the cell chip; and arranging a cell slice in the reaction tank. A virus infection cell slice is prepared through the following steps: 1, preparation of the substrate: preparing the substrate by adopting organic macromolecular materials, metals or silicon, and fabricating the reaction tank on the substrate surface by adopting a micro-processing technology; 2, preparation of the cell slice: preparing the virus infection cell slice or a pathogenic microorganism cell slice according to detected pathogenic microorganism; 3, preparation of the cell chip: adhering the cell slice in the reaction tank. The invention provides the pathogenic microorganism infection diagnosis type cell chip which has the characteristics of high flux, low cost, rapidness and accuracy, and a preparation method thereof.

Description

technical field [0001] The invention relates to a production procedure of a pathogenic microorganism infection diagnosis type cell chip. Background technique [0002] Infectious diseases are common and frequently-occurring diseases that endanger people's health and life. The prevalence of infection and the complexity of pathogenicity make the clinical diagnosis and treatment of this kind of disease very difficult, and an effective and rapid diagnostic technique is urgently needed. For the detection of viral disease pathogens, due to the difficulty in culturing the virus, the time is long, the positive rate is low, and it is not easy to operate, so the clinical diagnosis of virus infection mainly relies on serological methods and molecular biological methods. However, the above two types of methods have shortcomings or deficiencies to varying degrees. For example: neutralization test, complement fixation test, and hemagglutination inhibition test have limitations such as ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 王爽
Owner DALIAN CHUANGDA TECH TRADE MARKET