Use of peptide for imparting body taste
A thick flavor imparting agent and concentration technology, applied in the field of thick flavor imparting agent and compound thick flavor imparting agent, to achieve the effect of excellent stability
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[0050] In addition, in the complex flavor-imparting agent of the present invention, the amino acid or peptide used in combination with γ-Glu-Abu may have γ-Glu-X-OCH(Z)CO 2 Peptide derivatives of the structure of H. Here, X in the formula represents an amino acid or an amino acid derivative, and Z represents H (hydrogen atom) or CH 3 (methyl). In addition, in the above formula γ-Glu-Val-Y, Y is a compound of GlyA or LacA. As specific examples, γ-Glu-Val-GlyA, γ-Glu-tLeu-GlyA, γ-Glu-Abu-GlyA, γ-Glu-Val-LacA, γ-Glu-tLeu-LacA and γ-Glu- Abu-LacA et al. Furthermore, GlyA means glyoxylic acid, and LacA means lactic acid. Lactic acid may be either S-type or R-type, preferably S-type. The structural formulas of these compounds are as follows:
[0051] chemical formula 1
[0052]
[0053] chemical formula 2
[0054]
[0055] chemical formula 3
[0056]
[0057] chemical formula 4
[0058]
[0059] chemical formula 5
[0060]
[0061] chemical formula 6
[00...
Embodiment
[0141] Preparation of various samples
[0142] γ-L-Glu-L-Abu (Abu: α-aminobutyric acid, Bachem Feinchemikalien AG), γ-L-Glu-L-Ala (Bachem Feinchemikalien AG), γ-L-Glu-L-Cys (Sigma- Aldrich Japan Co., Ltd.), γ-L-Glu-L-Thr (Kokudo Chemical Co., Ltd.) and other four kinds of purchased products were used.
[0143] Synthesis of γ-L-Glu-L-Val
[0144] (Step 1) Z-L-Glu-OBzl (N-α-benzyloxycarbonyl-L-glutamic acid α-benzyl ester, 7.619g, 20.51mmol) and Val-OBzl·HCl (L-valine benzyl ester salt Salt, 5 g, 20.51 mmol) was dissolved in dichloromethane (100 ml), and the solution was kept at 0°C. Triethylamine (3.2ml, 22.57mmol), HOBt (1-hydroxybenzotriazole, 3.053g, 22.57mmol) and WSC·HCl (1-ethyl-3-(3-dimethylaminopropyl) Carbodiimide hydrochloride, 4.326 g, 22.57 mmol) was added to the solution and stirred overnight at room temperature. The reaction solution was concentrated under reduced pressure, and the residue was dissolved in ethyl acetate (220 ml). The solution was partitio...
Embodiment 1
[0178] Example 1 Evaluation of rich taste imparting activity
[0179] For γ-Glu-Abu, the strength of the kokumi-imparting activity was examined by a quantitative sensory evaluation experiment.
[0180] The quantitative sensory evaluation experiment was implemented as follows. In distilled water containing sodium glutamate (0.05g / dl), inosinic acid (0.05g / dl), sodium chloride (0.5g / dl), the test compound was mixed at 0.001 ~ 0.5g / dl In this case, the strength of the kokumi-imparting activity was determined. After the sample was dissolved, the sample that was acidic to the non-added control was used after adjusting the pH to a range of ±0.2 relative to the non-added control with NaOH. For the sensory evaluation, control: 0 points, strong: 3 points, very strong: 5 points, and n=4 were implemented. In addition, in order to clarify the scale more clearly, the first taste and the middle aftertaste of 0.001 g / dl γ-Glu-Val-Gly were each taken as 3.0 points. In addition, the so-c...
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