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Composition for alleviating ultraviolet radiation-induced damage

A composition, ultraviolet technology, applied in the fields of drug combination, food science, drug delivery, etc., can solve problems such as skin damage

Inactive Publication Date: 2012-05-30
SHISEIDO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since UV-A is not absorbed by the ozone layer, UV-A accounts for most of the ultraviolet rays reaching the ground, causing skin damage

Method used

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  • Composition for alleviating ultraviolet radiation-induced damage
  • Composition for alleviating ultraviolet radiation-induced damage
  • Composition for alleviating ultraviolet radiation-induced damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] UV damage reducing effect of methionine

[0062] method

[0063] cell culture

[0064] As the cells, commercially available human neonatal dermal fibroblasts (Cryo NHDF-Neo, Sanko Pure Chemical Industries, Ltd.) were used. The cells were 2 x 10 5Cells / mL were inoculated into a commercially available 35mm diameter petri dish (BD FALCON 353001, Becton Dickinson, Japan), and a commercially available cell culture medium (D-MEM (1 g / L glucose), Wako Pure culture medium (hereinafter referred to as "normal medium") supplemented with 10% fetal bovine serum. The cells can be cultured in the normal medium supplemented with 1% antibiotic (15240-062, GIBCO). The cells were incubated at 37°C, 5% CO 2 and incubated under saturated water vapor conditions for about 24 hours.

[0065] After that, the medium used for culturing the cells was replaced with the addition of 1×10 -3 % BSO (L-buthionine-(S,R)-sulfoximine, Wako Pure Chemical Industries), which is a biosynthesis inhibitor...

Embodiment 2

[0086] UV damage reducing effect of serine

[0087] method

[0088] Cell culture, amino acid addition before ultraviolet irradiation, ultraviolet irradiation, amino acid addition after ultraviolet irradiation, and quantification of cell damage were performed in the same manner as in Example 1. at 7.5 or 9J / cm 2 Irradiate ultraviolet (UV-A). When studying the effect of adding serine before ultraviolet irradiation (hereinafter referred to as "addition of serine before irradiation".) and adding serine after irradiation with ultraviolet light (hereinafter referred to as "addition of serine after irradiation".), 0.0001 to 100 μM of L-serine (197 -00403, Wako Pure Chemical Industries Ltd.) and D-serine (197-08823, Wako Pure Chemical Industries Ltd.). The effect of adding serine after irradiation was evaluated by irradiating cells with 7.5 J / cm 2 After 21 hours of UV-A, the cells were returned to the normal medium and cultured for 21 hours, and D-serine was added to the cultured ...

Embodiment 3

[0099] UV damage reducing effect of D-cycloserine

[0100] method

[0101] Cell culture, amino acid addition before ultraviolet irradiation, ultraviolet irradiation, amino acid addition after ultraviolet irradiation, and quantification of cell damage were performed in the same manner as in Example 1. at 9J / cm 2 Irradiate ultraviolet (UV-A). D-cycloserine (C6880, Sigma) at 0.0001 to 100 μM was used to study the effect of adding D-cycloserine (hereinafter referred to as “addition of D-cycloserine before irradiation”) before ultraviolet irradiation.

[0102] Result of adding D-cycloserine before irradiation

[0103] Figure 7 Shown to investigate the effect of adding D-cycloserine before irradiation on irradiation by UV-A 9J / cm 2 Experimental results of UV-induced damage to fibroblasts. The error bar of each experimental condition represents the standard deviation of the measured value of the experimental result repeated 3 to 4 times under the same condition. In addition, ...

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Abstract

Disclosed are a composition for alleviating ultraviolet radiation-induced damage, which is stable and safe and can be used on a day-to-day basis, and a medicinal composition, an external skin preparation, a food composition and a medicine for cataract, each comprising said composition for alleviating ultraviolet radiation-induced damage. Specifically disclosed is a composition for alleviating ultraviolet radiation-induced damage which contains one or more kinds of compounds selected from the group consisting of methionine, D-serine and derivatives and / or salts of the same. The aforesaid composition may be an external skin preparation, an anti-wrinkle agent, a sunscreen agent, a medicinal composition for treating and / or preventing skin diseases, a food composition or a medicine for cataract. In the aforesaid composition for alleviating ultraviolet radiation-induced damage, said methionine may be D-methionine, and said D-serine derivative may be D-cycloserine.

Description

technical field [0001] The present invention relates to an ultraviolet damage reducing composition containing one or more compounds selected from the group consisting of methionine and D-serine and their derivatives and / or salts; The resulting skin disease and a method for improving the cosmetic state of the skin, the method comprising the step of administering one or more compounds selected from the group consisting of methionine and D-serine and their derivatives and / or salts . Background technique [0002] Ultraviolet rays can be classified into long wavelength region ultraviolet rays (UV-A) longer than about 320 nm, medium wavelength region ultraviolet rays (UV-B) ranging from about 320 nm to about 280 nm, and short wavelength region ultraviolet rays (UV-C) shorter than about 280 nm. Among them, since UV-C is absorbed by the ozone layer, the sunlight reaching the ground does not contain UV-C. UV-A and UV-B are both contained in sunlight reaching the ground, but UV-B is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/198A23L1/305A61K8/44A61P17/00A61P17/16A61P35/00A61P37/02A61P37/08A61Q17/04
CPCA61K9/0014A61K9/12A61K9/7007A61K9/4858A61K8/44A61Q19/004A61K8/0208A61K9/107A23V2002/00A61K31/198A61K9/2018A61Q17/04A61Q19/08A23L1/3051A23L33/175A61P17/00A61P17/16A61P27/12A61P35/00A61P37/02A61P37/08A23V2200/318A23V2250/0632A23V2250/0642
Inventor 芦田丰东条洋介水本智惠子三田真史
Owner SHISEIDO CO LTD
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