Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Adjuvant composition comprising (poly-gamma-glutamate)-chitosan nanoparticles

A technology of chitosan nanometer and glutamic acid, which can be used in drug combinations, medical preparations containing active ingredients, microorganisms, etc., can solve the problem of not yet inducing immune response and so on.

Inactive Publication Date: 2012-05-30
BIOLEADERS CORP +4
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Complexes of poly-γ-glutamic acid and chitosan nanoparticles are ionically bonded poly-γ-glutamic acid and chitosan complexes, and are used as carriers for orally delivered insulin or vehicles for DNA delivery, but have not yet Its application to induce immune response is reported (Lin, Y. et al., Biomacromolecules, 6: 1104, 2005; Lin, Y. et al., Nanotechnology, 16: 105102, 2007)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Adjuvant composition comprising (poly-gamma-glutamate)-chitosan nanoparticles
  • Adjuvant composition comprising (poly-gamma-glutamate)-chitosan nanoparticles
  • Adjuvant composition comprising (poly-gamma-glutamate)-chitosan nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: Preparation of ultra-high molecular weight poly-γ-glutamic acid

[0060] Prepared as a basal medium for the production of poly-γ-glutamic acid (supplemented with 3% L-glutamic acid; containing 3% glucose, (NH 4 ) 2 SO 4 1%, KH 2 PO 4 0.27%, Na 2 HPO 4 12H 2 O 0.17%, NaCl 0.1%, Sodium Citrate 0.5%, Soybean Peptone 0.02%, MgSO 4 ·7H 2 O 0.7%, vitamin solution 10ml / L, PH 6.8) and sterilized. Inoculate the medium (LB medium) of Bacillus subtilis var.chungkookjang (Bacillus subtilis var.chungkookjang) (KCTC 0697BP) into the medium of a 5L fermenter (working volume 3L) with a concentration of 4%, and stir with 500rpm, 1vvm Aeration rate and 37°C fermentation for 48 hours. Bacterial cells were then removed using a small filter press (1% diatomaceous earth), and the remaining material was used as a sample solution containing poly-γ-glutamic acid.

[0061] The pH of the sample solution containing poly-γ-glutamic acid was adjusted to 2.0 with a 2N sulfu...

Embodiment 2

[0062] Embodiment 2: Preparation of poly-γ-glutamic acid-chitosan nanoparticles

[0063] Nanoparticles as an adjuvant were prepared using poly-γ-glutamic acid and chitosan (Amicogen Co., Ltd., Korea) prepared in Example 1.

[0064]Specifically, poly-γ-glutamic acid and chitosan were dissolved in 0.85% NaCl solution. The poly-gamma-glutamic acid solution and the chitosan solution are mixed with each other at a ratio of 1:1-8:1 (poly-gamma-glutamic acid:chitosan), thereby preparing the surface negatively charged poly-gamma-glutamic acid Acid-chitosan nanoparticles. The particle size and surface charge of the prepared nanoparticles were measured using DLS (Dynamic Light Scattering). As a result, it can be seen that the prepared nanoparticles have a particle size of 200-300 nm and a surface charge of -20.8 mV. In addition, the surface morphology of the as-prepared nanoparticles was observed with an electron microscope (see figure 1 ).

[0065] [Table 1] Particle size and surf...

Embodiment 3

[0067] Example 3: Preparation of Poly-γ-Glutamate-Chitosan Nanoparticles Using Multiple Sequences of Addition of Target Proteins

[0068] In order to verify that the poly-γ-glutamic acid-chitosan nanoparticles prepared in Example 2 are used as an adjuvant to enhance the function of producing antibodies to the corresponding protein, the pI value of the corresponding protein is determined, and a variety of the protein is used Sequence of additions to prepare nanoparticles. First, OVA-FITC having a pI value of 5.2 obtained by bonding a fluorescent substance FITC to OVA protein (Sigma Corporation, USA) was bonded to polyγ-glutamic acid-nanoparticles. Specifically, the following two kinds of nanoparticles were prepared: those prepared by mixing poly-γ-glutamic acid and OVA-FITC, and then adding chitosan thereto, and those prepared by mixing chitosan and OVA-FITC, and then adding Nanoparticles prepared by adding poly-γ-glutamic acid. The degree of bonding of OVA in the prepared na...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to an adjuvant composition comprising (poly-gamma-glutamate)-chitosan nanoparticles and a vaccine composition comprising the adjuvant composition, and more specifically to an adjuvant composition comprising nanoparticles by an ionic bond between (poly-gamma-glutamate) of proven safety and chitosan and a vaccine composition comprising the (poly-gamma-glutamate)-chitosan nanoparticles and an antigen. The adjuvant composition comprising (poly-gamma-glutamate)-chitosan nanoparticles according to the present invention has little or no toxicity and side effects and is advantageous in increasing the rate of antibody production when added to the vaccine for virus and bacterial infections and to the vaccine for prevention and treatment of cancer in humans or animals.

Description

technical field [0001] The present invention relates to the adjuvant composition that comprises poly-gamma-glutamic acid-chitosan nano particle and the vaccine composition that comprises described adjuvant composition, more particularly, the present invention relates to comprise by chitosan and have safe guarantee An adjuvant composition of nanoparticles prepared by ion bonding between poly-gamma-glutamic acid and a vaccine composition comprising the poly-gamma-glutamic acid-chitosan nano-particles and an antigen. Background technique [0002] Adjuvants are materials that can be used to produce vaccines with increased antigenicity or for therapeutic and prophylactic purposes by increasing nonspecific immune responses to antigens. Since adjuvants are used to maintain a strong and rapid immune response to an antigen over a longer period of time when antigen levels are low, these adjuvants can be used in vaccine preparations. In addition, adjuvants enable the use of specific a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/145A61P31/16A61P35/00
CPCA61K2039/55583C12N2760/16134A61K39/145A61K39/39A61K39/12A61K2039/543A61P29/00A61P31/00A61P31/12A61P31/16A61P35/00A61P37/04
Inventor 成文喜夫厦玲金哲仲崔荣基林容泽丁栋镇沈尚武
Owner BIOLEADERS CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com