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Application of recombinant human Prx-6 protein in treating burns and scalds

A recombinant protein, prx-6 technology, applied in the field of biotechnology and medicine, can solve problems such as lack of burns and scalds

Inactive Publication Date: 2012-06-06
李建远
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these studies were mainly about the anti-oxidative damage effect of Prx-6 protein in heart, lung or skin tissue, and did not involve the use of Prx-6 protein in the treatment of burns and scalds
[0008] In summary, there is still a lack of satisfactory medicines for the treatment of burns and scalds, so there is an urgent need in this area to develop new medicines that can be used for the treatment of burns and scalds.

Method used

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  • Application of recombinant human Prx-6 protein in treating burns and scalds
  • Application of recombinant human Prx-6 protein in treating burns and scalds
  • Application of recombinant human Prx-6 protein in treating burns and scalds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Construction of pET32b(+) / Prx-6 expression vector

[0050] A pair of specific primers (upstream primer: 5'-tatccatatgcccggaggtctgcttc-3' (SEQ ID NO: 3), downstream primer: 5'-ttactcgagaggctggggtgtgtagcg-3' (SEQ ID NO: 4)), the enzyme cutting sites are NdeI and XhoI respectively,

[0051] Human epididymis mRNA extracted by conventional methods was used as a template for RT-PCR amplification. see results figure 1 , a 688bp amplified fragment consistent with the theoretical value was detected.

[0052] The amplified fragments were separated and digested with NdeI and XhoI. Then, the double-digested fragment was ligated into the pET32b(+) expression vector (purchased from Novagen) that had been linearized with NdeI and XhoI, and transformed into conventional Escherichia coli TOP10F' (purchased from Invitrogen). The positive clones were screened by colony PCR, and the correctness of the sequence and expression reading frame was further confirmed by sequencing,...

Embodiment 2

[0053] Example 2. Expression of Prx-6 protein

[0054] The plasmid pET32b(+) / Prx-6 was transformed into Escherichia coli Origami B(DE3) (purchased from Novagen), and positive clones were inoculated in LB medium containing 100ug / ml ampicillin, and cultured overnight at 37 degrees in a shaker. The next day, transfer to LB medium containing 100ug / ml ampicillin at a ratio of 1:100, culture on a 37-degree shaker until the bacterial density OD600=0.6-0.8, and then add IPTG to a final concentration of 0.4mM to induce the target protein Prx-6 After 3-4 hours, the cells were collected by centrifugation.

Embodiment 3

[0055] The purification of embodiment 3.Prx-6 protein

[0056] The bacteria collected by centrifugation were resuspended in buffer A (20mM phosphate buffer, 150mM sodium chloride, pH7.2), ultrasonically disrupted in an ice bath, centrifuged at 20,000g at low temperature (4°C) for 15min, and the supernatant was taken as the purified sample. After equilibrating the nickel affinity chromatography column with buffer A, put the sample on the column, then wash the column with buffer A containing 50mM imidazole to remove impurity protein (peak 1), and elute the target protein with buffer A containing 300mM imidazole down (peak 2) (see figure 2 ).

[0057] Finally, the eluted protein was replaced with buffer A by G-25 desalting column to remove imidazole, and detected by SDS-PAGE electrophoresis. After analysis, the Prx-6 protein with a purity of more than 95% and a molecular weight of about 26.1 kD was obtained. (See image 3 ).

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Abstract

The invention provides application of recombinant human Prx-6 protein in treating burns and scalds. Specifically speaking, the invention provides application of recombinant human Prx-6 protein in preparation of medicines used for treating burns and scalds. According to results of experiments in the invention, Prx-6 protein plays an important role in promoting wound healing in the process of treatment of burns and scalds.

Description

technical field [0001] The invention relates to the fields of biotechnology and medicine, in particular to the use of recombinant human Prx-6 protein in treating burns and scalds. Background technique [0002] Burns and scalds are commonly encountered diseases, frequently-occurring diseases, and also traumatic diseases with higher disability rate. The light ones also bring great pain to the wounded, and the severe ones often damage the organ function, causing great inconvenience to the work and life of the patient. Therefore, for moderate and mild burns / scalds, the healing period should be shortened as much as possible, the pain of the patient should be reduced, and the formation of scars should be reduced as much as possible. For patients with severe burns and scalds, complications such as various organ injuries and secondary infections caused by reperfusion should be minimized. [0003] At present, the methods commonly used in clinical treatment of burns are usually band...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61P17/02
CPCA61K9/0014A61K38/44
Inventor 李建远
Owner 李建远
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