Active biological recombiner and preparation method thereof

A bio-composite agent and compound agent technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of low decomposition rate, high preparation cost, unchanged natural properties, etc., and achieve environmental tolerance. Strong elasticity, large span of stain resistance, and the effect of solving weak stain resistance

Inactive Publication Date: 2012-06-06
SHANGHAI YULEI ENVIRONMENTAL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its preparation cost is high, production capacity is low, liquid bacterial agent has a short validity period in the engineering interface, poor stability, harsh storage conditions, and excessive transportation consumption in engineering applications
Lack of industrialization prospects, the most fatal flaw is that the natural properties of the strains of the liquid bacterial agent remain unchanged, and cannot fundamentally solve the problems of weak stain resistance, poor environmental tolerance and low decomposition rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The present invention is further described as an example with Gao Shi No. 1 formula below:

[0040] The amount of raw materials and auxiliary materials (according to the percentage of ingredients)

[0041] 1. Sake yeast liquid: 6% (bacteria content 4×10 10 )

[0042] 2. Streptomyces griseus liquid: 6% (bacteria content 4×10 10 )

[0043] 3. Rice bran 85%

[0044] 4. Water balance

[0045] Gao's No. 1 medium formula combination: (%)

[0046] Potassium nitrate 1.0%

[0047] Potassium dihydrogen phosphate 0.5%

[0048] Magnesium Sulfate 0.5%

[0049] Ferrous sulfate 0.001%

[0050] Soluble Starch 2%

[0051] Agar powder 2%

[0052] Wort 93.999%

[0053] Preparation of Active Biological Complex:

[0054] 1. Preparation of sake yeast strains: First, take 940ml of wort culture medium and 940ml of Gaoshi No. 1 medium and pack them into 9 bottles of 100ml triangular flasks at 0.15mpa, control the temperature at 121, and sterilize for 15 minutes to obtain sterilized...

Embodiment 2

[0061] A preparation method of an active biological compound, characterized in that the method comprises the following steps:

[0062] (1) Preparation of sake yeast liquid:

[0063] a. Preparation of liquid medium: select wort medium as nutrient source, sterilize at 0.15mpa, 121°C for 15 minutes to obtain sterilized malt medium, wherein the wort medium is mixed according to the following components and contents Obtained: potassium nitrate 0.5kg, potassium dihydrogen phosphate 0.25kg, magnesium sulfate 0.25kg, ferrous sulfate 0.0005kg, soluble starch 1kg, agar powder 1kg, wort juice 98kg;

[0064] b. Preparation of the strains in the triangular flask: take the slanted sake yeast strains and insert them into the triangular flask containing the sterilized malt medium in a sterile environment, and cultivate them for 48 hours at 34°C with a vibration rate of 100rpm to obtain the strains of the triangular flask liquid;

[0065] c, the preparation of production bacterial classificati...

Embodiment 3

[0074] A preparation method of an active biological compound, the method comprising the following steps:

[0075] (1) Preparation of sake yeast liquid:

[0076] a. Preparation of liquid medium: select wort medium as nutrient source, sterilize at 0.15mpa, 121°C for 15 minutes to obtain sterilized malt medium, wherein the wort medium is mixed according to the following components and contents Obtained: potassium nitrate 1.5kg, potassium dihydrogen phosphate 0.75kg, magnesium sulfate 0.75kg, ferrous sulfate 0.0015kg, soluble starch 3kg, agar powder 3kg, wort juice 90kg;

[0077] b. Preparation of the strains in the triangular flask: take the slanted sake yeast strains and insert them into the triangular flask containing the sterilized malt medium in a sterile environment, and cultivate them for 48 hours at 34°C with a vibration rate of 200rpm to obtain the strains of the triangular flask liquid;

[0078] c, the preparation of production bacterial classification: insert in the f...

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PUM

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Abstract

The invention relates to an active biological recombiner and a preparation method thereof. Raw materials of the recombiner comprise the following components, by weight: 0.3-08% of sake yeast liquid, 0.3-08% of streptomyces griseus liquid, 70-90% of rice bran and the balance of water. A maltose medium is utilized to culture the sake yeast liquid and the streptomyces griseus liquid; then the raw materials are mixed and fermented to obtain the active biological recombiner. Compared with a prior art, the active biological recombiner of the invention is cultured by using rice bran as a main inocula carrier and sake yeast and streptomyces griseus as main starters, can degrade natural organic pollutants in the environment, has advantages of low manufacture cost, high production power, long period of validity, strong stability, simple storage conditions, convenience for transportation, composite compatibility of different bacteria, large pollution resistance span and large environment tolerance elasticity.

Description

technical field [0001] The invention relates to an active agent for degrading organic pollutants and a preparation method thereof, in particular to an active biological compound agent and a preparation method thereof. Background technique [0002] It is known that the bioaugmentation (introduction) process that has emerged internationally is based on the principle of using biological culture methods to select a single bacterial species, prepare it into a liquid preparation with a high bacterial content, and put it into the engineering interface to strengthen the biodegradation process. strength. However, its preparation cost is high, production capacity is low, the validity period of the liquid bacterial agent in the engineering interface is short, the stability is poor, and the storage conditions are harsh, and the transportation consumption of the engineering application is too large. The lack of industrialization prospects, the most fatal flaw is that the natural propert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02C12N1/16C12N1/20C12R1/85C12R1/545
Inventor 冯坤范
Owner SHANGHAI YULEI ENVIRONMENTAL BIOTECH
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