Preparation method and use of soluble squid ink melanin

A technology of melanin and squid ink is applied in the application of antioxidant activity, in the field of preparing soluble melanin, and achieves the effects of high application potential and economy, simple preparation process and high content
CN102499389AInactive Publication Date: 2012-06-20ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2012-06-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of soluble squid ink melanin. The preparation method comprises the following steps of: 1) washing ink suspension in the ink sac of a squid with ice water at the temperature of 0-4 DEG C, centrifugating and then removing supernatant fluid to obtain a precipitate; 2) performing vacuum freeze-drying on the precipitate obtained in the step 1) to obtain an insoluble melanin crude product; 3) dissolving the insoluble melanin crude product in alkali liquor and performing ultrasonic processing for 30-90 minutes, wherein the material-liquid ratio of the insoluble melanin crude product to the alkali liquor is 1g of the insoluble melanin crude product / 20-80ml of the alkali liquor, and the alkali liquor is an NaOH solution in the molar concentration of 0.1-1.0mol / L; and 4) centrifugating the product obtained in the step 3), taking the supernatant fluid and performing vacuum freeze-drying to obtain the soluble squid ink melanin. The invention further provides a use of the soluble squid ink melanin as an antioxidant.
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Description

technical field

[0001] The invention relates to a method for preparing melanin, in particular to a method for preparing soluble melanin from squid ink, and the application of the antioxidant activity thereof. Background technique

[0002] Squid is a common name for squid and squid among cephalopod marine animals. North Pacific squid is one of the most important squid processing species, the annual processing volume is between 150,000 and 300,000 tons, and its ink sac accounts for about 1.3% of the whole body weight. A large amount of waste generated after squid processing, especially the ink sac, has not been well utilized, and has also caused great pollution to the environment. At the same time, squid ink contains a variety of biologically active substances such as polypeptide fragments, acidic polysaccharides, tyrosinase, etc. Therefore, the extraction of melanin from squid ink not only improves the comprehensive utilization rate of waste in the squid processing process,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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