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Electrode surface modification material, preparation method thereof and application thereof

An enzyme electrode and general formula technology, applied in the field of electrode surface modification materials and its preparation and application, can solve the problems of affecting the electrochemical performance of the electrode, limited pH value range, increasing the preparation steps, etc., and achieve the effect of good redox conductivity

Active Publication Date: 2013-06-12
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, when used in biosensors, in order to increase the repeatable stability of the enzyme electrode, it is still necessary to perform cross-linking to immobilize the biological enzyme, which not only increases the preparation steps but also affects the electrochemical performance of the electrode itself; at the same time, based on osmium metal complexed polyethylene Pyridine-based modified electrodes have a limited pH range for detecting solutions, usually less than 7

Method used

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  • Electrode surface modification material, preparation method thereof and application thereof
  • Electrode surface modification material, preparation method thereof and application thereof
  • Electrode surface modification material, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, preparation cell-g-P4VP-Os

[0050] Weigh 0.1 g of hydroxypropyl cellulose with a weight average molecular weight of 80,000 and a degree of substitution of 3.5, add it to 50 mL of deionized water, blow nitrogen, and dissolve completely in a constant temperature oil bath at 40 degrees Celsius. Regulate the pH of the solution to 1.5 with concentrated nitric acid, add 2 milliliters of 4-vinylpyridine (1.96 g), stir to make it fully dissolve, then add 5 mL of initiator ceric ammonium nitrate aqueous solution (0.008 g / ml), continue nitrogen, and then Seal and react for 2 hours. The reaction was terminated with hydroquinone. The reaction mixture was precipitated, filtered, dialyzed, and dried to obtain a cellulose derivative grafted polyvinylpyridine copolymer with a weight average molecular weight of 900,000 (measured by gel permeation chromatography, and the mobile phase was N,N-dimethylformamide) .

[0051] Weigh 10 mg of cellulose derivative grafted polyv...

Embodiment 2

[0055] Embodiment 2, preparation cell-g-P4VP-Os

[0056] Weigh 0.1 g of hydroxypropyl cellulose with a weight average molecular weight of 80,000 and a degree of substitution of 3.5, add it to 50 mL of deionized water, blow nitrogen, and dissolve completely in a constant temperature oil bath at 40 degrees Celsius. Regulate the pH of the solution to 1.5 with concentrated nitric acid, add 0.5 milliliters of 4-vinylpyridine (0.49 g), stir to make it fully dissolve, then add 5 mL of initiator ceric ammonium nitrate aqueous solution (0.008 g / ml), continue nitrogen, and then React with seal for 6 hours. The reaction was terminated with hydroquinone. The reaction mixture is precipitated, filtered, dialyzed, and dried to obtain a cellulose derivative grafted polyvinylpyridine copolymer with a weight average molecular weight of 100,000 (measured by gel permeation chromatography, and the mobile phase is N,N-dimethylamide).

[0057] Weigh 10 mg of cellulose derivative-grafted polyvinylp...

Embodiment 3

[0059] Embodiment 3, preparation of glucose oxidase electrode modified by cell-g-P4VP-Os

[0060] (1), 25 mg of cell-g-P4VP-Os prepared in Example 1 was fully dissolved in 5 ml of deionized water, and prepared into a solution with a concentration of 5 g / L;

[0061] (2), weigh 50 milligrams of glucose oxidase (enzyme activity > 100U / mg), fully dissolve in 5 milliliters of deionized water, prepare a solution with a concentration of 10 grams per liter, and refrigerate for later use;

[0062] (3) Appropriate amounts of the solutions of steps (1) and (2) were respectively taken to form a mixed solution at a volume ratio of 1:1, and fully mixed evenly. The mixed solution was drip-coated on the surface of the working electrode, and dried naturally to form a film to obtain a cell-g-P4VP-Os modified glucose oxidase electrode.

[0063] The above-mentioned glucose oxidase electrode was tested for electrical performance, and the results of cyclic voltammetry scanning were as follows: fi...

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Abstract

The invention discloses an osmium metal complexed cellulose derivative grafted polyvinylpyridine copolymer, a preparation method thereof and an application thereof. The structural formula of the copolymer is represented by formula (I). The osmium metal complexed cellulose derivative grafted polyvinylpyridine copolymer provided in the invention, which has a good redox conducting characteristic, can be used as an electrode surface modification material for a biosensor and the like. By using the material of the invention to modify an enzyme electrode, the repetition stability of the electrode isimproved, and the suitable pH value range of a detection solution is enlarged. No crosslinking steps are needed when an enzyme is fixed by the electrode modification material of the invention, so thematerial can be simply and conveniently used to prepare the biosensor. Taking a glucose oxidase electrode for sample, the suitable pH value of the detection solution is enlarged to 5-13 when the glucose oxidase electrode is used to detect the concentration of glucose.

Description

technical field [0001] The invention relates to an electrode surface modification material and a preparation method and application thereof. Background technique [0002] In the past 20 years, the research in the field of chemically modified electrodes has received great attention at home and abroad. It represents a new concept of the electrode / electrolyte interface, which is not only of great significance in the study of electrochemical theory, but also has a wide range of potential applications. Prospects, such as for bioanalysis and biosensors, etc. [0003] Among various modified electrode materials, polymer materials have been widely studied and applied because of their stability, high efficiency, and sensitivity. Such polymers generally have redox and conductive properties, which are used to regulate the electron transfer process between the electrode and the target solution. Such redox polymers mainly include two types according to the positions of the redox groups,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F251/02C08F8/42G01N27/327
Inventor 康宏亮刘瑞刚黄勇
Owner INST OF CHEM CHINESE ACAD OF SCI
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