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Method for producing crocin with higher than 95% purity from gardenia

A technology of saffron and gardenia, which is applied in the direction of chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems affecting the application of saffron active substances and the difficulty of realizing industrial production, etc., achieve low cost and improve column efficiency , the effect of improving the chromatography efficiency

Active Publication Date: 2012-06-27
QIANJIANG GREEN SEA TREASURE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology is difficult to realize industrial production, which directly affects the application of the active substance saffron

Method used

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  • Method for producing crocin with higher than 95% purity from gardenia
  • Method for producing crocin with higher than 95% purity from gardenia
  • Method for producing crocin with higher than 95% purity from gardenia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Extraction: Crush 1 kg of dried gardenia fruit to 10-40 mesh, soak in 8-15 kg of pure water for 1 hour at room temperature, then infiltrate at an appropriate flow rate, share 25-35 kg of pure water for percolation and extraction, 4 -8 hours percolation extraction is complete, to obtain the extract A.

[0023] 2. Microfiltration: pass the extract A through a 0.2-1 micron ceramic membrane to obtain a clarified filtrate B.

[0024] 3. Sample loading and adsorption: Use 3 sections of ZT-02 macroporous adsorption resin columns, each column is 70 ml, and the clarified filtrate B is first applied to the first column for adsorption. Connect the end of the second column, and continue to absorb until the effluent of the second column is reddish, string the third column C at the end of the second column, until the effluent of column C is reddish, remove the column C . At the end of the second column, connect column D again, and handle the same method as column C, and so on un...

Embodiment 2

[0029] 1. Extraction: Grind 10 kg of dried gardenia fruit to 10-40 mesh, soak in 80-150 kg of pure water for 1 hour at room temperature, then infiltrate at an appropriate flow rate, share 250-350 kg of pure water for percolation and extraction, 4 -8 hours percolation extraction is complete, to obtain the extract A.

[0030] 2. Microfiltration: pass the extract A through a 0.2-1 micron ceramic membrane to obtain a clarified filtrate B.

[0031] 3. Sample loading and adsorption: use 3 sections of ZT-02 macroporous adsorption resin columns, each column is 1000 ml, firstly put the clarified filtrate B on the first column for adsorption, until the effluent is reddish, the Connect the end of the second column, and continue to absorb until the effluent of the second column is reddish, string the third column C at the end of the second column, until the effluent of column C is reddish, remove the column C . At the end of the second column, connect column D again, and handle the same...

Embodiment 3

[0036] 1. Extraction: Crush 100 kg of dried gardenia fruit to 10-40 mesh, soak in 800-1500 kg of pure water at room temperature for 1 hour, then infiltrate at an appropriate flow rate, share 2500-3500 kg of pure water for percolation extraction, 4 -8 hours percolation extraction is complete, to obtain the extract A.

[0037]2. Microfiltration: pass the extract A through a 0.2-1 micron ceramic membrane to obtain a clarified filtrate B.

[0038] 3. Sample loading and adsorption: use 3 sections of ZT-02 macroporous adsorption resin columns, each column is 10 liters, put the clarified filtrate B on the first column for adsorption, until the effluent is reddish, the Connect the end of the second column, and continue to absorb until the effluent of the second column is reddish, string the third column C at the end of the second column, until the effluent of column C is reddish, remove the column C . At the end of the second column, connect column D again, and handle the same metho...

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Abstract

The invention relates to a method for producing crocin with higher than 95% purity from gardenia. The invention adopts a normal-temperature water-percolation extraction process, can effectively reduce the extraction of pectins, proteins and other impurities, and avoids difficulty in the subsequent separation and purification. Before extraction, seed crushing is avoided in the gardenia fruit pulverizing process, thereby reducing the extraction of oil. Water extract microfiltration can remove solid particles, pectins and other impurities in the water extract and filter out bacteria and other microbes, and the microfiltered water extract can be directly continuously adsorbed by a macroporous resin column. Fine macroporous resin is used instead of conventional large-particle-diameter macroporous resin for adsorption chromatography, normal pressure technology is upgraded to pressurization technology, and a continuous circulation chromatography method is used instead of the traditional linear chromatography method, thereby enhancing the column efficiency and chromatography efficiency; and in cooperation with the refinement of the C18 column, the pure crocin can be directly prepared. The whole technological process is simple, has the advantages of solvent saving, low cost, high efficiency and environmental protection, and is suitable for industrial production of the crocin product of which the purity is higher than 95%.

Description

technical field [0001] The invention relates to a method for producing crocin with a purity greater than 95% by using Gardenia jasmine as a raw material, and belongs to the field of extraction, separation and purification of natural products. Background technique [0002] The ripe fruit of Gardenia contains about 0.5% crocin, also known as α-crocin and crocin-1, which is a water-soluble carotenoid compound with strong free radical scavenging, antioxidant and anticancer activities. The preparation of pure saffron is difficult, even the standard product purchased from Sigma has a purity of less than 95%. At present, saffron is often extracted in the laboratory by water or ethanol extraction, repeated chromatography on a macroporous adsorption resin column and a silica gel column, gradient elution, and then recrystallization to obtain pure saffron. The prior art is difficult to realize industrialized production, which directly affects the application of the active substance cr...

Claims

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Application Information

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IPC IPC(8): C07H13/06C07H1/08
Inventor 袁晓王国艳叶巍周东斌林顺权杨威曹健林志芬林立克杨毅融
Owner QIANJIANG GREEN SEA TREASURE BIO TECH
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