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Method for preparing Sphaeranthanolide

A technology of trichome and oxaloside, applied in the field of medicine, can solve the problems such as extraction and separation of high-purity oxaloside that have not yet been found.

Inactive Publication Date: 2012-06-27
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are few researches on incineratorin, and there is no research report on the extraction and isolation of high-purity incineratorin from natural plants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take 1kg of Asteraceae tomentosa flowers as raw material, crush them, use 2L of acetone solution to ultrasonically extract twice, recover acetone from the extract to an appropriate volume, add 1200g of diatomaceous earth and stir evenly while it is hot, pack it into a column, and first elute the fat-soluble oil with petroleum ether. Substances were eluted with ethyl acetate, and the ethyl acetate eluate was collected and concentrated into an extract; mixed n-hexane-ethyl acetate-methanol-water with a volume ratio of 4:3:8:3, oscillated and shaken, Make the two phases reach equilibrium, separate after standing and stratifying, the lower phase is the mobile phase, and the upper phase is the stationary phase, respectively degas them by ultrasonic degassing for 40 minutes and then cool them for later use; take the extract and dissolve them in equal volumes of the upper and lower phase solvents , filter to obtain the sample solution, fill the prepared stationary phase with a ...

Embodiment 2

[0015] Take 1kg of Asteraceae tomentosa flowers as raw materials, crush them, and use 10L of acetone solution to ultrasonically extract 3 times, recover acetone from the extract to an appropriate volume, add 300g of diatomaceous earth and stir evenly while it is hot, pack it into a column, and first use petroleum ether to elute the fat-soluble Substances were eluted with ethyl acetate, and the ethyl acetate eluate was collected and concentrated into an extract; mixed n-hexane-ethyl acetate-methanol-water with a volume ratio of 6:5:7:3, oscillated and shaken, Make the two phases reach equilibrium, separate after standing and stratifying, the lower phase is the mobile phase, and the upper phase is the stationary phase, respectively degas them by ultrasonic degassing for 40 minutes and then cool them for later use; take the extract and dissolve them in equal volumes of the upper and lower phase solvents , filter to obtain the sample solution, fill the prepared stationary phase wit...

Embodiment 3

[0017] Take 5kg of Asteraceae flora as a raw material, crush them, and use 80L of acetone solution to ultrasonically extract 3 times, recover the acetone from the extract to an appropriate volume, add 2400g of diatomaceous earth and stir evenly while it is hot, pack it into a column, and first use petroleum ether to elute the fat-soluble Substances were eluted with ethyl acetate, and the ethyl acetate eluate was collected and concentrated into an extract; mixed n-hexane-ethyl acetate-methanol-water with a volume ratio of 5:4:7:2, oscillated and shaken, Make the two phases reach equilibrium, separate after standing and stratifying, the lower phase is the mobile phase, and the upper phase is the stationary phase, respectively degas them by ultrasonic degassing for 40 minutes and then cool them for later use; take the extract and dissolve them in equal volumes of the upper and lower phase solvents , filter to obtain the sample solution, fill the prepared stationary phase with a fl...

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PUM

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Abstract

The invention discloses a method for preparing Sphaeranthanolide. The method is characterized in that the method comprises the following steps: taking Sphaeranthus indicus L flowers which are treated as raw materials, crushing the flowers, extracting the flowers by acetone, carrying out acetone recovery on the obtained extract to a proper volume, adding diatomite, uniformly stirring, filling them to a column, eluting fat-soluble substances by petroleum ether, eluting by ethyl acetate, collecting the obtained ethyl acetate eluate, concentrating the eluate to an extractum, and purifying the extractum by adopting high speed countercurrent chromatography and liquid chromatography to obtain the Sphaeranthanolide with the purity of higher than 98%. The method of the invention has the advantages of large preparation amount, less product loss, high purity, easy realization of large scale, and the like.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for preparing astroside. Background technique [0002] Pleurotus officinalis, alias: Ma Lagan; Latin name: Sphaeranthus indicus L. It is derived from the dicotyledonous plant medicine Asteraceae tomentosa, mainly distributed in the southwest of Yunnan. The plant has the effects of drying dampness, relieving itching, expelling wind and dampness. Indications: skin ringworm, rheumatic arthralgia. Sexually pungent, warm. poisonous. Go into lung, liver two warps. When used externally, crush it for external application. The plant has long been used as an important medicinal plant in India to treat skin diseases, as a hemostatic and anthelmintic for stomach ailments, and to treat swollen glands, bronchitis, jaundice, and depression. [0003] Sesquiterpenoids are important bioactive components of a large class of medicinal plants, which are widely found in ...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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